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Based on the comprehensive protein engineering platform established through years of experiences, scientists form Creative Biostructure can provide advanced custom Mempro™ annexins production services using cell-based expression system.
Mempro™ cell-based protein production system is widely used for the production of membrane proteins. Annexins are a well-known group of membrane binding proteins, which are Ca2+-regulated phospholipid binding proteins. Members of annexin family contain four conserved structural elements termed annexin repeats composed of approximately 70 amino acid residues. Annexin repeat is highly α-helical and slightly curved disc that has a surface containing the Ca2+- and membrane-binding sites. It has been reported that annexins play important roles in vesicle transport (such as exocytosis and endocytosis) and calcium ion channel formation. Additionally, annexins can function as membrane scaffolding proteins to anchor other proteins to the cell membrane.
Figure 1. The structural model of annexin A1. (J. Cell Sci., 2004)
Creative Biostructure can produce high-yield annexins using cell-based protein expression system, we can employ various strategies for Mempro™ cell-based protein production, including:
Escherichia coli (E. coli) is the most commonly used bacterial host for the production of annexins . Yeast cells (such as saccharomyces cerevisiae and pichia pastoris) system, combines prokaryotic as well as eukaryotic characteristics. It is a powerful eukaryotic host due to single cells, fast growth rates, inexpensive media, high cell densities. Insect cells (such as Sf9, Sf21 and High Five) are also the major system for annexins expression, which are developed from transfected insect cells in combination with vectors derived from the baculovirus species AcMNPV. Mammalian cells present another eukaryotic system for annexins expression, which provide near-native cell environment. Cell lines derived from COS, CHO, BHK-21, HEK293, HeLa and GH3 can be widely used for membrane protein expression.
With the Mempro™ cell-based protein production platform, Creative Biostructure is capable of expressing, isolating, purifying and crystallizing annexins to facilitate the study of their physiological functions.
We provide other various Mempro™ membrane protein production services. Please feel free to contact us for a detailed quote.
C. Trometer, and P. Falson (2010). Mammalian membrane protein expression in baculovirus-infected insect cells. Methods Mol. Biol., 601: 105-117.
F. Junge, et al. (2008) Large-scale production of functional membrane proteins. Cellular Mol. Life Sci., 65 (11): 1729-1755.
J. Petschnigg, et al. (2011). Using yeast as a model to study membrane proteins. Curr. Opin. Nephrol. Hypertens., 20(4): 425-432.
U. Rescher and V. Gerke (2004). Annexins - unique membrane binding proteins with diverse functions. J. Cell Sci., 117: 2631-2639.
V. Gerke and S. E. Moss (2002). Annexins: from structure to function. Physiol. Rev., 82(2): 331-371.