Creative Biostructure provides unmatched custom Mempro™ immunoglobulin-like superfamily (E set) protein production services using cell-based expression system.
Mempro™ cell-based protein production system is the most widely used for membrane protein production. Immunoglobulin superfamily (IgSF) is a variety class of cell surface proteins that are involved in the processes of recognition, binding, and adhesion to cells. Members of IgSF contain a sturctural domain termed Ig domain, which possess around 70-110 amino-acid residues. It has been shown that Ig domain has a characteristic Ig-like fold composed of a beta-sandwich of seven or more strands in two sheets of antiparallel beta strands (greek key). Ig-like fold is involved in interactions with other Ig-like domains via its beta-sheets. The "early" set (E set) domains are possibly associated with the immunoglobulin and/or fibronectin type III Ig-like protein superfamilies. Up to now, It has been known that Ig-like E set superfamily consists of five families, including ML domain, RHO protein GDP dissociation inhibitor, immunoprotective extracellular protein, gamma-adaptin C-terminal appendage domain-like and periplasmic glucan biosynthesis protein(MdoG).
Figure 1. The structural model of Rho GDP-dissociation inhibitor 1.
Creative Biostructure has strong expertise in native activity of E set protein production in cell-based protein expression system, we can provide various strategies for Mempro™ cell-based protein production, including:
Among Mempro™ cell-based protein production platform, Escherichia coli is the most commonly used bacterial host for the production of E set proteins. Yeast cell is another eukaryotic host, owing to the characteristics of yeast, such as single cell, fast growth rate, inexpensive media and high cell density. Insect cell (such as Sf9, Sf21 and High Five) is also a major system for E set protein expression. Mammalian cell presents another eukaryotic system for E set protein expression, which provides nearly native-like eukaryotic cell environment. Mammalian cell lines derived from COS, CHO, BHK-21, HEK293, HeLa and GH3 are commonly used for membrane protein expression.
With the Mempro™ cell-based protein production system, Creative Biostructure provides expressing, isolating, purifying and crystallizing E set proteins to facilitate the analysis of their biological functions.
Creative Biostructure can provide other various Mempro™ membrane protein production services. Please feel free to contact us for a detailed quote.
References:A. Bateman, et al. (1996). Members of the immunoglobulin superfamily in bacteria. Protein Sci., 5: 1939-1941.C. Trometer, and P. Falson (2010). Mammalian membrane protein expression in baculovirus-infected insect cells. Methods Mol. Biol., 601: 105-117.mmunoglobulin E-set (IPR014756). EMBL-EBI. (http://www.ebi.ac.uk/interpro/entry/IPR014756).Immunoglobulin superfamily. (https://en.wikipedia.org/wiki/Immunoglobulin_superfamily).J. Petschnigg, et al. (2011). Using yeast as a model to study membrane proteins. Curr. Opin. Nephrol. Hypertens., 20(4): 425-432.S. Schlegel, et al. (2013). Bacterial-based membrane protein production. Biochim. Biophys. Acta., 1843(8): 1739-1749.T. Lai, et al. (2013). Advances in Mammalian cell line development technologies for recombinant protein production. Pharmaceuticals, 6(5): 579-603.