Based on the comprehensive membrane protein production platform established through years of experience, scientists from Creative Biostructure is able to provide you best custom Mempro™ cell-free ion channel superfamily protein production services. Our patent cell-free expression platform can bypass the limitations that encountered in cell-based protein production system, such as host cell toxicity, low yield and solubilization and purification using detergents.
Ion channel is a type of pore-forming transmembrane protein that has a wide range of biological functions, such as regulating cell volume, setting up a resting membrane potential, forming action potentials and other electrical signals by gating the flow of ions across the cell membrane, controlling the flow of ions across secretory and epithelial cells, etc. Ion channels exist in the membranes of all cells, which are considered to be one of the two traditional classes of ionophoric proteins, with the other class known as ion transporters, including the sodium-potassium pump, sodium-calcium exchanger, and sodium-glucose transport proteins, and amongst others. It is reported that the open-state of an ion channel facilitates the movement of ions into or out of the cell in single-file manner. In addition, individual ion channels are specific to particular ions, meaning that they usually allow only a single type of ion to pass through them.
Figure 1. Schematic of ion channel. (access from Nature Education)
Creative Biostructure is highly-experienced in offering various strategies for high-quality ion channel superfamily proteins, including:
• Mempro™ Ion Channel Superfamily Production in Bacterial ExtractE. coli S30 extract is popularly used for membrane protein production. Mempro™ membrane protein expression by bacterial extract provides you the enhancement of expression of desired membrane proteins, and easy to perform scale-up membrane protein production.
• Mempro™ Ion Channel Superfamily Production in Wheat GermCreative Biostructure provides eukaryotic translation system for ion channel superfamily proteins production in wheat germ system. This system facilitates the membrane proteins performing correct folding and post-translational modifications and maintaining the correct structural and functional characteristics.
• Mempro™ Ion Channel Superfamily Production in Rabbit ReticulocyteCrude rabbit reticulocyte lysate is the excellent system for eukaryotic membrane protein production, providing multiple options for the optimization of the production protocols, such as the availability of materials, protein origin and complexity, downstream processing, etc.
• Mempro™ Ion Channel Superfamily Production in BaculovirusBaculovirus expression system from Autographa californica nucleopolyhedrovirus (AcNPV) is also a powerful system for transmembrane protein expression, which is developed in the early stage that transfected insect cells in combination with vectors derived from the baculovirus.
Creative Biostructure can also provide Mempro™ plant-based virus-like particles (VLPs) production services, Mempro™ animal-free recombinant protein production services and Mempro™ membrane protein production services. Please feel free to contact us for a detailed quote.
References:F. Bernhard, et al. (2013). Cell-free expression - making a mark. Cur. Opin. Struct. Biol., 23: 374-380.Ion channel. (https://en.wikipedia.org/wiki/Ion_channel)Ion channel. Scitable by Nature Education. (http://www.nature.com/scitable/topicpage/ion-channel-14047658)