With years of experience in the comprehensive membrane protein production platform, scientists from Creative Biostructure can offer tailored Mempro™ hypoxia-induced proteins production services using detergent-free protein expression system.
Hypoxia-induced proteins contain gene 2 protein (HIG2), carbonic anhydrase 9 (CA9/CAIX), carbonic anhydrase XII (CAXII) and so on, which are all developed in the hypoxic microenvironments. CAIX is regarded as one of the most optimal cellular biomarkers of hypoxia and has turned into a special therapeutic target in the clinical trials of cancers. It’s shown that CAIX obviously increases in low oxygen environment of renal cell carcinomas (RCCs). The hypoxia inducible factors (HIF) acts as a crucial role in the modulation of membrane-bound proteins CAIX and CAXII. In addition, HIG2 is a 7 kD trans-membrane protein which is expressed during the process of tumor evolution. All these indicated that hypoxia-induced proteins play an important role in therapeutics and pharmaceutics.
Figure 1. A schematic showing the CA XII dimer in the membrane. (PNAS, 2001)
Creative Biostructure has ample professional experience in high quality hypoxia-induced proteins production using detergent-free membrane protein expression system, we can perform various strategies for Mempro™ detergent-free protein production, including:
Due to the structural characteristics, nanodiscs can magically make membrane proteins stabilized. They are composed of membrane scaffold proteins (MSPs) which can offer two surface, one is a hydrophilic at the outside, while the other is facing the lipids. Nanodiscs are generally used for structural, enzymatic and biophysical researches.
Amphipols (APols) are projected to maintain transmembrane proteins (TMPs) solvable in aqueous solutions without detergent, they are synthesized by forming amide bonds between octylamine and, somewhile sopropylamine. The wide resources of polymer chemistry can make it possible to tailor the physicochemical properties ofamphipols toward peculiar utilities in biophysics and biochemistry.
This method generates SMA lipid particles (SMALPs) in which a little disk of lipid bilayer revolved around by polymer encircles the membrane proteins. In addition, electron microscopy has quickly progressed, which results in the highly development of data acquisition, together with the conformational and resolution information getable.
Creative Biostructure has been pioneered in the detergent-free expression system for a long time, we can provide world-leading production services of Hypoxia-induced proteins.
Creative Biostructure provides other various Mempro™ membrane protein production services. Please feel free to contact us for a detailed quote.
References: Neumeister, V. M., et al. (2012). Hypoxia-induced protein CAIX is associated with somatic loss of BRCA1 protein and pathway activity in triple negative breast cancer. Breast cancer research and treatment, 136(1), 67-75. Norris, M. L., & Millhorn, D. E. (1995). Hypoxia-induced protein binding to O2-responsive sequences on the tyrosine hydroxylase gene. Journal of Biological Chemistry, 270(40), 23774-23779. Postis, V., Rawson, S., Mitchell, J. K., Lee, S. C., Parslow, R. A., Dafforn, T. R., . . . Muench, S. P. (2015). The use of SMALPs as a novel membrane protein scaffold for structure study by negative stain electron microscopy. Biochimica et Biophysica Acta (BBA)-Biomembranes, 1848(2), 496-501.