Based on the comprehensive protein engineering platform established through years of experience, scientists from Creative Biostructure offers tailored Mempro™ lipoxygenases production services using detergent-free expression system.
Figure 1. The structural of rabbit reticulocyte 15s lipoxygenase (Proteins 2008).
Creative Biostructure has rich professional experience in high quality lipoxygenases production using detergent-free membrane protein expression system, we can perform various strategies for Mempro™ detergent-free protein production, including:
A nanodisc can magically make membrane proteins stabilized, due to its structural characteristics, the membrane proteins in aqueous solutions were also solubled by nanodisc. In contrast with bicelles, detergent micelles and liposomes, a more native environment where proteins remain monodisperse and active can be offered.
Amphipols are soluble proteins that can keep membrane proteins soluble in water without common surfactants. Amphipols were synthesized by forming amide bonds between octylamine and, sometimes, isopropylamine.
SMALPs are disc like structures containing a protein in a lipid bilayer, by using this approach, there is no need to sift special detergents, the speed and economy of protein extraction can be significantly improved. In addition, the use of SMALPs can make detergent-free isolation of membrane proteins and retention of their crude lipid environment available.
These novel detergent-free technologies for lipoxygenases production can be obtained easily, and enabling more comprehensively structural and functional studies.
Creative Biostructure provides other various Mempro™ membrane protein production services. Please feel free to contact us for a detailed quote.
References:L. Kathy et al. (2015). Dissecting the Structure of Membrane Proteins. Genetic Engineering & Biotechnology News 35 (17): 16. J.Bowie et al. (2001). Stabilizing membrane proteins. Current Opinion in Structural Biology 11 (4): 397–402. M. Gaczynska, et al. (1994). Peptidase activities of proteasomes are differentially regulated by the major histocompatibility complex-encoded genes for LMP2 and LMP7. Proc. Nat. Acad. Sci. U. S. A., 91(20): 9213-9217.M. Jamshad, et al. (2011). Surfactant-free purification of membrane proteins with intact native membrane environment. Biochem. Soc. Trans., 39: 813-818. Lipoxygenases.