Based on the comprehensive protein engineering platform established through years of experience, scientists from Creative Biostructure offers tailored Mempro™ major facilitator superfamily production services using detergent-free expression system.
The major facilitator superfamily (MFS) enables to accelerate the movement of diminutive solutes get through cell membranes, so they can response to chemiosmotic gradients. However, MFS is a class of membrane transport proteins, which are expressed widely in all part of life for the import or export of target substrates. In the premier researches, scientists regarded the MFS family as proteins mainly uptake of sugars, while subsequent studies showed that oligosaccharides, amino acids, metabolites, drugs and oxyanions are all in the region MFS transported. The MFS zealously control transport through two approaches: one is serve as the uniporter by using electrochemical gradient of the target substrate, the other one is combined to the movement of a second substrate as a cotransporter.
The crystal structures of a quantity of MFS transporters have been characterized. The initial structures of the major facilitator superfamily which have been solved were glycerol 3-phosphate/phosphate exchanger GlpT and the lactoseproton symporter LacY. These contribute to clarify the overall structure of the protein family and provide initial models for understanding the MFS transport mechanism.
Figure 1. Crystal Structure of Lactose Permease LacY. (Microbiol 1998)
Creative Biostructure has rich professional experience in high quality Major Facilitator Superfamily (MFS) Production using detergent-free membrane protein expression system, we can perform various strategies for Mempro™ detergent-free protein production, including:
Nanodiscs provide stable membrane proteins and through this approach the membrane protein target is solubilized. Moreover, they are under the existence of phospholipids and a surrounding amphipathic helical protein belt which named membrane scaffold protein (MSP). The length of the MSP is the crucial factor of the size when the target membrane protein synchronously assembles with phospholipids into a discoidal bilayer.
Amphipols are a new class of surfactants, they are synthesized by forming amide bonds between octylamine and, sometimes sopropylamine. Besides, amphipols enable to stabilize in aqueous solution with their inartificial nature state four well-authenticate integral membrane proteins.
SMALPs are disc like structures including a protein in a lipid bilayer, parceled by the amphipathic styrene maleic acid polymer (SMA). This SMA molecule cuts discs from the cell membrane of nearly 11 nm in diameter. There are masses of obvious advantages by using SMALPs for structural determination.
Creative Biostructure provides other various Mempro™ membrane protein production services. Please feel free to contact us for a detailed quote.
Pao SS, et al. (1998). Major Facilitator Superfamily. Microbiol. 62: 1-34.
W. Jump up, et al. (1998). Sugar transporters from bacteria, parasites and mammals: structure-activity relationships. Trends Biochem. 23: 476-481.
H. Bayburt, et al. (2010). Membrane protein assembly into Nanodiscs. FEBS letters. 84: 1721-1727.
M. Jamshad, et al. (2011). Surfactant-free purification of membrane proteins with intact native membrane environment. Biochem. 39: 813-818.