Creative Biostructure has developed a first-class membrane protein production platform through years of experience, we can provide advanced and tailored Mempro™ galactose-binding domain-like protein production services based on our plant-derived expression system.
Creative Biostructure is highly-experienced in producing 1) animal-free, 2) low endotoxin and 3) low protease activity recombinant membrane proteins. Most notably, recombinant membrane proteins expressed by our plant system are free of animal components, serum, endotoxins and antibiotics as well as human or animal infectious agents or other endogenous mammalian contamination. Animal-free recombinant membrane proteins are particularly crucial for customers concerned with experimental variables caused by trace animal components or mammalian pathogens.
Creative Biostructure can offer membrane protein production services based on various types of plants, such as Nicotiana benthamiana (tobacco), Medicago rativa (alfalfa), Arabidopsis thaliana (A. thaliana), potato, maize, barley and lettuce.
Proteins possessing a galactose-binding domain-like fold can be found in different protein families, in both eukaryotes and prokaryotes. It is well-known that galactose-binding domain is composed of a beta-sandwich, in which the strands making up the sheets exhibit a jellyroll fold. The members of this family share a high degree of similarity in the beta-sandwich, despite an often low level of sequence similarity. The common function of galactose-binding domains is to bind with specific ligands, such as cell-surface-attached carbohydrate substrates for galactose oxidase and membrane-anchored ephrin for the Eph family of receptor tyrosine kinases. Additionally, the galactose-binding domain-like proteins are involved in carbohydrate metabolic process.
Figure 1. The structural model of coagulation factor VIII, light chain. (OPM Database)
With the Mempro™ plant-based protein production platform, Creative Biostructure is capable of expressing, isolating, purifying and crystallizing galactose-binding domain-like proteins to facilitate the study of their structural and biological functions. Misfolding, aggregation, inactivity, poor stability, high endotoxin and solubility, etc. are the common difficulties encountered in cell-based expression system. These difficulties can be bypassed in the renewed plant-based expression system. Furthermore, post-translational modification such as glycosylation and disulfide bonds can be introduced in plant system.
Creative Biostructure can also provide Mempro™ plant-based virus-like particles (VLPs) production services, Mempro™ animal-free recombinant protein production services and Mempro™ membrane protein production services. Please feel free to contact us for a detailed quote.
References:A. Wiktorek-Smagur, et al. (2012). Green way of biomedicine – how to force plants to produce new important proteins. Transgenic Plants-Advances and Limitations, Chapter 3. doi: 10.5772/1409.F. Junge, et al. (2008) Large-scale production of functional membrane proteins. Cellular Mol. Life Sci., 65 (11): 1729-1755.Galactose-binding domain-like (IPR008979). (http://www.ebi.ac.uk/interpro/entry/IPR008979)Galactose-binding domain-like protein. (http://www.uniprot.org/uniprot/K2RZ34)