Creative Biostructure offers high-quality and high-yield glycolipid transfer proteins (GLTPs) using plant-based expression system. Our plant-based expression system holds broad advantages for membrane protein production, such as high yield, animal-free, low protease activity, and low endotoxin, etc.
Creative Biostructure has strong professional experience in providing best membrane protein production services using various types of plant hosts, including Nicotiana benthamiana (tobacco), Medicago rativa (alfalfa), Arabidopsis thaliana (A. thaliana), potato, maize, barley and lettuce.
Glycolipid transfer proteins (GLTPs) are a group of peripheral proteins (approximately 24 kDa) existing in many species that catalyzes the transfer of glycolipids through the intracellular membranes, but not the phospholipids or neutral lipids to transfer between different membranes. It is well-known that both sphingoid- and glycerol-based glycolipids can be transferred by GLTP as long as the hydrophobic lipid backbone has beta-linked initial sugar. The glycolipids transfer of GLTP is composed of several steps as follows: GLTP binds to the membranes; GLTP scans the membrane and uptakes the glycolipids; and then extracts the lipid from the intracellular membrane. Notably, GLTP transfers the glycosphingolipids, and this process play a pivot regulation function in various cell processes such as cell growth, surface adhesion, and neurodevelopment.
Figure 1. Putative membrane interaction region of GLTP, showing the location of the three tryptophan residues (red color). (Biochim. Biophys. Acta, 2007)
Creative Biostructure can perform two different options for membrane protein production in plants: transient expression and stable transformation. We can produce animal-free recombinant membrane proteins, which are particularly crucial for customers concerned with experimental variables caused by trace animal components or mammalian pathogens. Recombinant membrane proteins expressed by our plant system are free of animal components, serum, endotoxins and antibiotics as well as human or animal infectious agents or other endogenous mammalian contamination. In addition, post-translational modification such as glycosylation and disulfide bonds can be introduced in plant system.
With the Mempro™ plant-based protein production system, Creative Biostructure is capable of expressing, isolating and purifying GLTPs to facilitate the study of their biological functions. We can overcome the common difficulties encountered in cell-based expression system, such as misfolding, aggregation, inactivity, high endotoxin, poor stability and solubility, etc.
Creative Biostructure can also provide Mempro™ plant-based virus-like particles (VLPs) production services, Mempro™ animal-free recombinant protein production services and Mempro™ membrane protein production services. Please feel free to contact us for a detailed quote.
References:A. Wiktorek-Smagur, et al. (2012). Green way of biomedicine – how to force plants to produce new important proteins. Transgenic Plants-Advances and Limitations, Chapter 3. doi: 10.5772/1409.Glycolipid transfer protein. (https://en.wikipedia.org/wiki/Glycolipid_transfer_protein)R. E. Brown and P. Mattjus (2007). Glycolipid transfer proteins. Biochim. Biophys. Acta., 1771(6): 746-760.