Creative Biostructure now provides custom Mempro™ transmembrane proteins with NAD(P)-binding Rossmann-fold domains production service using virus-like particles for various membrane protein researches.
The transmembrane proteins with NAD(P)-binding Rossmann-fold domains superfamily is a membrane protein superfamily that contains two families: flavin-containing amine oxidoreductase family and tyrosine-dependent oxidoreductases family. Flavin-containing amine oxidoreductase family contains multiple amine oxidases such as L-amino acid oxidases (LAO), maize polyamine oxidase (PAO), antibacterial glycoproteins, tryptophan 2-monooxygenase, putrescine oxidase, corticosteroid-binding proteins, etc. These amine oxidases are involved in the metabolisms of amine. Tyrosine-dependent oxidoreductases family, also called short-chain dehydrogenases/reductases (SDR) family, is a large protein family that consists of enzymes catalyzing NAD(P)(H)-dependent oxidation/reduction reactions. About 140 members of this family have been identified from various species. Despite their residue identity is as low as 15-30%, their 3D folds are quite similar from each other, except the substrate-binding regions.
Figure 1. The schematic structure of human monoamine oxidase A. (OPM Database)
Virus-like particles (VLPs) are known as a powerful technique for various biological and bio-medical research programs. There is a long history for VLPs used in vaccine developments. What's more, VLPs are also widely performed for antigen delivery, membrane protein production, and heterologous epitope presentation. VLPs are highly organized subunits that are assembled by viral structural proteins. VLPs and their native viruses behave the same in many ways. However, unlike virus, VLPs are non-infectious because of lacking viral genetic materials. Various virus families have been reported to produce VLPs successfully such as Parvoviridae, bacteriophages, Retroviridae, etc.
With years of experience in membrane protein production, Creative Biostructure now can provide high-yield and high purity Mempro™ transmembrane proteins with NAD(P)-binding Rossmann-fold domains production service using our most advanced VLPs technique. Multiple host systems are available for meeting different requirements in each cases, including green plants, bacteria, yeasts, insect cells and mammalian cells. We will proceed the co-expression of viral core structural protein and target protein in host cells. The expressed core structural protein will perform the self-assembly in the plasmid membrane and then bud-off from the plasmid membrane to form the lipoparticles (VLPs that contain high concentration of specific membrane proteins in their native conformation), during which the target transmembrane proteins with NAD(P)-binding Rossmann-fold domains will be captured within the lipolarticles. Lipoparticles then can be obtained at the surface of host cells with the target protein being protected perfectly within them.
Creative Biostructure also provides other various kinds of Mempro™ membrane protein production services with high quality using advanced VLPs technique. Welcome to contact us for more details.
ReferenceFilling C, Berndt K D, Benach J, et al. Critical residues for structure and catalysis in short-chain dehydrogenases/reductases[J]. Journal of Biological Chemistry, 2002, 277(28): 25677-25684.Federico R, et al. Characterization of maize polyamine oxidase. Phytochemistry. 1990; 29(8):2411-4.A.Roldão, et al. Virus-like particles in vaccine development.Expert Rev. Vaccines, 2010,9(10): 1149-1176.Feussner I, Wasternack C. The lipoxygenase pathway [J]. Annual review of plant biology, 2002, 53(1): 275-297.