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To probe cell biology at the molecular level and to visualize single molecule activity with macroscopic cellular behavior in real time, novel super-resolution far-field microscopy (or nanoscopy) approaches have been developed. Stimulated emission depletion microscopy with gated detection (gSTED) is one of the most advanced techniques that allow the acquisition of high-resolution images by point scanning over the sample, generating images pixel by pixel.
In gSTED, a diffraction-limited spot is excited at a certain wavelength; and a super-imposed, red-shifted, second laser beam projects to deplete a donut-shaped area, leaving only a central focal spot with a dimension below the diffraction limit. The size of the center focal spot can be tuned by changing the intensity of the depletion laser such that a lateral resolution of less than 50 nm could be achieved. As gSTED utilizes pulsed excitation in combination with continuous wave (CW) laser depletion and time-gated detection, an important prerequisite is to use CW lasers with low noise; because any fluctuations in the power level compromise the performance of this technique.
The superior resolution gSTED has several features over conventional confocal microscopy:
Creative Biostructure provides gSTED services with the following advantages:
Creative Biostructure promises to work closely with our customers to provide excellent services. Please feel free to contact us for more information or a detailed quote.