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Super-resolution Stimulated Emission Depletion Microscopy with Gated Detection (gSTED) Service

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To probe cell biology at the molecular level and to visualize single molecule activity with macroscopic cellular behavior in real time, novel super-resolution far-field microscopy (or nanoscopy) approaches have been developed. Stimulated emission depletion microscopy with gated detection (gSTED) is one of the most advanced techniques that allow the acquisition of high-resolution images by point scanning over the sample, generating images pixel by pixel.

In gSTED, a diffraction-limited spot is excited at a certain wavelength; and a super-imposed, red-shifted, second laser beam projects to deplete a donut-shaped area, leaving only a central focal spot with a dimension below the diffraction limit. The size of the center focal spot can be tuned by changing the intensity of the depletion laser such that a lateral resolution of less than 50 nm could be achieved. As gSTED utilizes pulsed excitation in combination with continuous wave (CW) laser depletion and time-gated detection, an important prerequisite is to use CW lasers with low noise; because any fluctuations in the power level compromise the performance of this technique.

The superior resolution gSTED has several features over conventional confocal microscopy:

  • Better stability and reliability
  • Full spectrum of visible light and optimal color correction
  • Improved resolution
  • Deep live imaging and improved signal to noise ratios

Creative Biostructure provides gSTED services with the following advantages:

  • Professional scientific team
  • State-of-the-art technologies
  • Highly reliable and reproducible result
  • Competitive price in the market

Creative Biostructure promises to work closely with our customers to provide excellent services. Please feel free to contact us for more information or a detailed quote.

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References

  1. Wegel E, et al. Imaging cellular structures in super-resolution with SIM, STED and Localisation Microscopy: A practical comparison. Scientific Reports. 2016, 6(1): 1-13.
  2. Eggeling C, Honigmann A, Schulze M. gSTED Microscopy with an OPSL: Cutting Edge Super‐Resolution: Breaking the diffraction limit with a CW OPSL. Optik & Photonik. 2012, 7(2): 44-46.
  3. Vicidomini G, et al. STED nanoscopy with time-gated detection: theoretical and experimental aspects. PloS One. 2013, 8(1): e54421.

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