Welcome! For price inquiries, please feel free to contact us through the form on the right side. We will get back to you as soon as possible.
Fluorescence recovery after photobleaching (FRAP) is a versatile and widely accessible technique to investigate the kinetics of molecular in living cells, which has become an important tool for cell biologists.
FRAP experiment is performed under fluorescence microscopy in three steps. First, an initial fluorescence of fluorescent molecules is measured in the region of interest (ROI). Then, the fluorescent molecules are rapidly photobleached by focusing the high-intensity laser beam onto the defined area. Finally, the exchange of bleached molecules with unbleached molecules from the surrounding region is followed over time using a low-intensity laser. The time course of change in fluorescence intensity in the ROI after photobleaching is called FRAP curve, which yields information of both the recovery kinetics and the fraction of molecules free to diffuse.
Figure 1. FRAP diagram of a same cell in time series
FRAP has gained high recognition for several features:
FRAP can be applied to characterize various phenomena:
Creative Biostructure promises to work closely with our customers to provide excellent services. Please feel free to contact us for further information or a detailed quote.