Creative Biostructure can provide customized Mempro™ galactose-binding domain-like protein production services using cell-based expression system.
Mempro™ cell-based protein production system is the most widely used for the production of membrane proteins. The structure of galactose-binding domain consists of a beta-sandwich, in which the strands making up the sheets exhibit a jellyroll fold. The members of this family share a high degree of similarity in the beta-sandwich, despite an often low level of sequence similarity. Proteins possessing a galactose-binding domain-like fold can be found in both eukaryotes and prokaryotes. The common function of galactose-binding domains is to bind carbohydrate and have carbon-oxygen lyase activity, such as cell-surface-attached carbohydrate substrates for galactose oxidase and sialidase. Additionally, the galactose-binding domain-like proteins are involved in carbohydrate metabolic process.
Figure 1. Coagulation factor VIII, a galactose-binding domain-like protein. (OPM database)
Creative Biostructure produces high-yield galactose-binding domain-like proteins, we can perform various strategies for Mempro™ cell-based protein production, including:
Escherichia coli (E. coli) is the powerful bacterial host for the production of galactose-binding domain-like proteins. Yeast cells (such as saccharomyces cerevisiae and pichia pastoris) system, combines prokaryotic as well as eukaryotic characteristics. It is a perfect eukaryotic host due to single cells, fast growth rates, inexpensive media, high cell densities. Insect cells (such as Sf9, Sf21 and High Five) are also the major system for galactose-binding domain-like protein expression, which are developed from transfected insect cells in combination with vectors derived from the baculovirus species AcMNPV. Mammalian cells present another eukaryotic system for galactose-binding domain-like protein expression, which provide near-native cell environment. Cell lines derived from COS, CHO, BHK-21, HEK293, HeLa and GH3 can be widely used for membrane protein expression.
With the Mempro™ cell-based protein production platform, Creative Biostructure is capable of expressing, isolating, purifying and crystallizing galactose-binding domain-like proteins to facilitate the study of their physiological functions.
We provide other various Mempro™ membrane protein production services. Please feel free to contact us for a detailed quote.
References:C. Trometer, and P. Falson (2010). Mammalian membrane protein expression in baculovirus-infected insect cells. Methods Mol. Biol., 601: 105-117.F. Junge, et al. (2008) Large-scale production of functional membrane proteins. Cellular Mol. Life Sci., 65 (11): 1729-1755.Galactose-binding domain-like (IPR008979). (http://www.ebi.ac.uk/interpro/entry/IPR008979)Galactose-binding domain-like protein. (http://www.uniprot.org/uniprot/K2RZ34) J. Petschnigg, et al. (2011). Using yeast as a model to study membrane proteins. Curr. Opin. Nephrol. Hypertens., 20(4): 425-432.