Creative Biostructure has developed a comprehensive membrane protein production platform through years of experience. Our seasoned scientists can provide excellent custom membrane anchor helices of peripheral proteins production services using our innovative Mempro™ cell-free expression system, which can bypass the limitations that encountered in cell-based protein production system, such as host cell toxicity, low yield and solubilization and purification using detergents.
Membrane anchor helices of peripheral proteins belong to a large superfamily comprised of ten protein families, including membrane anchor helix of BAR domain, FATC domain, membrane-binding domain of phosphotransferase IIA component, anchor helix of protein yopD, anchor helix of glycosyl transferases group 1, heat shock protein 9/12, N-terminal helix of Disabled-2, lipid interacting helix of digalactosyldiacylglycerol synthase, N-terminal helix of Arf proteins and lipid droplet anchoring peptide. Members of membrane anchor helices of peripheral proteins play important roles in the peripheral membrane proteins' temporarily adhering to the plasma membrane or the penetrating the peripheral regions of the lipid bilayer.
Figure 1. Structural model of lipid droplet anchoring peptide of CGI-58. (OPM Database)
Creative Biostructure has strong experience in providing various strategies for high-quality membrane anchor helices of peripheral proteins, including:
• Mempro™ Membrane Anchor Helices of Peripheral Proteins Production in Bacterial ExtractE. coli S30 extract is the most widely used tool for membrane protein production. Mempro™ membrane protein expression by bacterial extract provides you the enhancement of expression of desired membrane proteins, and easy to perform scale-up membrane protein production.
• Mempro™ Membrane Anchor Helices of Peripheral Proteins Production in Wheat GermCreative Biostructure provides eukaryotic translation system for membrane anchor helices of peripheral proteins production in wheat germ system. This system facilitates the membrane proteins performing correct folding and post-translational modifications and maintaining the correct structural and functional characteristics.
• Mempro™ Membrane Anchor Helices of Peripheral Proteins Production in Rabbit ReticulocyteCrude rabbit reticulocyte lysate is the renewed system for eukaryotic membrane protein production, providing multiple options for the optimization of the production protocols, such as the availability of materials, protein origin and complexity, downstream processing, etc.
• Mempro™ Membrane Anchor Helices of Peripheral Proteins Production in BaculovirusBaculovirus expression system from Autographa californica nucleopolyhedrovirus (AcNPV) is also a powerful tool for protein expression, which is developed in the early stage that transfected insect cells in combination with vectors derived from the baculovirus.
Creative Biostructure can also provide Mempro™ plant-based virus-like particles (VLPs) production services, Mempro™ animal-free recombinant protein production services and Mempro™ membrane protein production services. Please feel free to contact us for a detailed quote.
References:F. Bernhard, et al. (2013). Cell-free expression - making a mark. Cur. Opin. Struct. Biol., 23: 374-380.D. Sakamuro, et al. (1996). BIN1 is a novel MYC–interacting protein with features of a tumour suppressor. Nature genetics, 14(1): 69-77.