Creative Biostructure can produce high-quality peptide fragments of water-soluble proteins by our unmatched Mempro™ cell-free protein production services. Our cell-free expression system can best preserve the native activity of peptide fragments of water-soluble proteins.
Peptide fragments of water-soluble proteins are a large group of peptides, which consist of ten families, including peptides of STE5 protein, prion protein peptides, peptides of viral protein R, peptides of huntingtin, peptides of lactotransferrin, amyloidogenic peptide of phosphatase, peptides of antifreeze protein, peptides of superoxide dismutase, hemocyanin-derived peptides and peptides of DNA-binding proteins. Viral protein R (Vpr) is human immunodeficiency virus type 1, which is a 14-kDa protein with 96 amino acid residues and is very crucial in the regulation of nuclear import of the HIV-1 pre-integration complex. Another member from peptide fragments of water-soluble proteins superfamily, amyloidogenic peptide of phosphatase, is in close association with Alzheimer's disease (AD) that is the accumulation of the amyloid-beta (Aβ) peptide in the form of amyloid plaques.
Figure 1. The structural model of TAR DNA-binding protein 43, peptide 307-349. (OPM Database)
Creative Biostructure can provide various strategies for high-quality Mempro™ cell-based peptide fragments of water-soluble proteins production, including: • Mempro™ Peptide Fragments of Water-soluble Proteins Production in Bacterial ExtractE. coli S30 extract is commonly employed for peptide fragments of water-soluble proteins production. Mempro™ protein expression by bacterial extract system provides you the enhancement of expression of the peptide fragments of membrane proteins, and easy to perform scale up the high-yield production.
• Mempro™ Peptide Fragments of Water-soluble Proteins Production in Wheat GermCreative Biostructure can provide eukaryotic translation system for membrane protein production in wheat germ system. This system facilitates the membrane proteins performing correct folding and post-translational modifications and maintaining the best structural and functional features.
• Mempro™ Peptide Fragments of Water-soluble Proteins Production in Rabbit ReticulocyteCrude rabbit reticulocyte lysate is the innovative host for eukaryotic membrane protein production, providing multiple options for the optimization of the production protocols, such as the availability of materials, protein origin and complexity, downstream processing, etc.
• Mempro™ Peptide Fragments of Water-soluble Proteins Production in BaculovirusBaculovirus expression system from Autographa californica nucleopolyhedrovirus (AcNPV) is also a major system for peptide fragments of water-soluble protein expression, which is developed in the early stage that transfected insect cells in combination with vectors derived from the baculovirus.
Creative Biostructure can also provide Mempro™ plant-based virus-like particles (VLPs) production services, Mempro™ animal-free recombinant protein production services and Mempro™ membrane protein production services. Please feel free to contact us for a detailed quote.
References:F. Bernhard, et al. (2013). Cell-free expression - making a mark. Cur. Opin. Struct. Biol., 23: 374-380. S. P. Braithwaite, et al. (2012). Protein phosphatases and Alzheimer's disease. Prog. Mol. Biol. Transl. Sci., 106: 343-379.