Creative Biostructure can provide unparalleled custom Mempro™ immunoglobulin-like superfamily (E set) protein production services using detergent-free expression system.
Immunoglobulin superfamily (IgSF) is a large group of membrane-bound proteins that are involved in the processes of recognition, binding, and adhesion to cells. Members of IgSF contain a sturctural domain termed Ig domain, which possess around 70-110 amino-acid residues. Immunoglobulin-like superfamily is also termed E set, including a wide range of domains that have an Ig-like fold in anti-parallel β-strands. Conventional approaches for E set protein production are performed through extraction from the membrane in the presence of detergent. However, detergent can induce conformational changes on membrane protein. Thus, stable solubilization of E set proteins is important issue in biotechnological field. Our alternative Mempro™ detergent-free production system can best preseve the native conformation of E set proteins for further biological studies.
Figure 1. A structural model of ganglioside GM2 activator.
Creative Biostructure have great expertise in high-yield E set protein production using detergent-free membrane protein expression system, we can perform various strategies for Mempro™ detergent-free E set protein production, including:
Nanodiscs are the self-assembly nanotechnology to stabilize membrane proteins removed from the membrane by membrane scaffold proteins (MSPs) in no need of detergent. Creative Biostructure can perform combination of cell-free expressed E set proteins with pre-assembled nanodiscs in absence of detergent.
The amphipathic amphipols have the ability to enwrap desired membrane proteins, allowing them to stay folded. Creative Biostructure can apply A8-35 to solubilize E set proteins during purification process.
The SMALPs are self-assembled by the simple addition of the SMA co-polymers. At neutral or alkaline pH, the disc-like structure assembles itself, encapsulating the E set proteins in a form amenable to be purified.
These novel detergent-free technologies for E set protein production can be obtained easily, and enabling more comprehensively structural and functional analyses.
Creative Biostructure can provide other various Mempro™ membrane protein production services. Please feel free to contact us for a detailed quote.
References:C. Tribet, et al. (1996). Amphipols: Polymers that keep membrane proteins soluble in aqueous solutions. Proc Natl Acad Sci U. S. A. 93: 15047-15050.G. Bodelón, et al. (2013). Immunoglobulin domains in Escherichia coli and other enterobacteria: from pathogenesis to applications in antibody technologies. FEMS Microbiol. Rev., 37(2): 204-250.Ig-like fold superfamily (E-set). EMBL-EBI. (http://pfam.xfam.org/clan/CL0159)Immunoglobulin superfamily. (https://en.wikipedia.org/wiki/Immunoglobulin_superfamily).M. Jamshad, et al. (2011). Surfactant-free purification of membrane proteins with intact native membrane environment. Biochem. Soc. Trans., 39: 813-818.