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Cryo-EM for Nanomaterials

Cryo Electronic Microscopy (Cryo-EM) has been extensively studied in life sciences due to it can provide the three-dimensional (3D) structure at a near-atomic resolutions. With the advance of microscope design, imaging hardware and image processing, Cryo-EM is increasingly becoming a mainstream technology for studying the structure of biological macromolecules. There is not required crystallization and only small amounts of sample are needed, Cryo-EM can present samples in a native state with imaging the sample’s intrinsic electron density, providing direct visualization of biological macromolecules, such as protein, nucleic acid, large complexes and so on.

Nanomaterials, usually means that materials of which a single unit is sized between 1 to 100 nm. Nanomaterials including the biological material (such as bacteria and viruses), some inorganic and organic compounds. Aqueous nanomaterials can form stable structure in the solvent, but after drying in the air, it has been great changes in the structure form. Therefore, in the use of conventional negative staining technique cannot get the real structure. For the unstable nanomaterials in a vacuum or in the air, due to there is no need a lot of particles and the complicated calculation, single particle Cryo-EM technology is relatively simple and can provide a structure in a native state, which makes Cryo-EM becoming an essential technology for studying the structure of nanomaterials.

At Creative Biostructure, we have a wealth of experience for studying the structures of biological nanomaterials by utilizing Cryo-EM, for example, the study of virus. With the most advanced high-resolution electron microscopy equipment and a direct electron detector in combination with enhanced image processing, 3D reconstruction and atomic model building, we can provide our customers with high quality, satisfying and excellent service.

High resolution 3D reconstruction of simulated T7MLDII/ “gold” particles.Figure 1. High resolution 3D reconstruction of simulated T7MLDII/ “gold” particles.

Creative Biostructure promises to work closely with our customers and provide the structure study of nanomaterials that our customers are interested in.

Please feel free to contact us for a detailed quote.

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References

  1. Eva Nogales, Sjors H.W. Scheres, Cryo-EM: A Unique Tool for the Visualization of Macromolecular Complexity. Mol Cell, 2015; 58:677-689.
  2. Guimei Yu, Rui Yan , et al. Single-Particle Cryo-EM and 3D Reconstruction of Hybrid Nanoparticles with Electron-Dense Components. Small, 2015; 11:5157-5163.
  3. Ramsey N. Majzoub, Kai K. Ewert, et al. Patterned Thread-like Micelles and DNA-Tethered Nanoparticles: A Structural Study of PEGylated Cationic Liposome-DNA Assemblies. Langmuir, 2015; 31:7073-7083.