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Cryo-EM for Filaments

Cryo-Electron Microscopy (Cryo-EM) techniques have been essential for understanding the structure of biological specimens such as cells, tissues and macromolecular complexes. Characterizing the molecular structure of macromolecules is crucial for getting an insight into understanding of the biochemical and cellular processes. Due to there is not required crystallization and only small amounts of sample are needed, Cryo-EM has become a major technique in structural biology for the studies of functional complexes. Meanwhile, with the advanced of image processing and three-dimensional reconstruction techniques, Cryo-EM can also provide a three-dimensional (3D) maps of biological macromolecules at a near-atomic resolutions.

In biology, a filament is a long chain of proteins, it can be found in hair, muscle, or in flagella. They are often bundled together for strength and rigidity, such as microfilaments, microtubules, myofilaments, and so on. Filaments are highly dynamic in nature, therefor it is difficult to obtain the structure by using the traditional X-ray crystallography. Here, at Creative Biostructure, we have a wealth of experience for studying the structure of biological macromolecules by utilizing Cryo-EM.

At Creative Biostructure, our service includes the preparation and purification of filaments, negative staining EM and image processing of Cryo-EM, provide the customer with the 3D reconstruction at a near atomic resolution, and finally provides a biologically insightful for understanding the biological progress.

High-resolution Cryo-EM structure determination of the hRAD51 presynaptic and postsynaptic filaments.Figure 1. High-resolution Cryo-EM structure determination of the hRAD51 presynaptic and postsynaptic filaments.

Creative Biostructure promises to work closely with our customers to provide excellent services of structural studies with unique biological insights.

Please feel free to contact us for a detailed quote.

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References

  1. Eva Nogales, Sjors H.W. Scheres, Cryo-EM: A Unique Tool for the Visualization of Macromolecular Complexity. Mol Cell, 2015; 58:677-689.
  2. Lingyun Zhao, Jingfei Xu, et al. Determining the RAD51-DNA Nucleoprotein Filament Structure and Function by Cryo-Electron Microscopy. Methods Enzymol, 2018; 600:179-199.
  3. Ahmet Mentesa, Andrew Huehn, et al. High-resolution cryo-EM structures of actin-bound myosin states reveal the mechanism of myosin force sensing. Proc Natl Acad Sci U S A. 2018; 115:1292-1297.