With years of experience in nanodiscs technology study, Creative Biostructure provides customized Mempro™ nanodiscs analysis service using free flow electrophoresis (FFE) technique. As a lead in the nanodiscs technology manufacture, Creative Biostructure offers high quality FFE technique services to meet client’s requirements.
FFE is a technique projected to separate the analytes in view of their electrophoretic mobility, which is available on both a preliminary scale and an analytical scale. The separation is accomplished in aqueous media in the lake of a matrix, therefore decreasing the loss of the material on account of unspecific combination or denaturing effects, which conversely leads to the probability of gaining high yields ( > 80%). Hence, FFE can be used either as an individual purification of assembled nanodiscs or combined with downstream applications or analysis. The sample is flowed into a planar flow chamber when it is separated. In this process, an electric field is vertically added to the direction of streaming, making analytes deflected based on their electrophoretic mobility when they go through the flow channel.
Figure 1. Free flow IZE for the separation of nanodiscs. (Justesen, B. H. 2014)
Creative Biostructure provides other various Mempro™ nanodisc technology services, besides nanodiscs analysis. Please feel free to contact us for a detailed quote.
Justesen, B. H. (2014). “Chromatographic and electrophoretic methods for nanodisc purification and analysis”. Reviews in Analytical Chemistry 33(3), 165-172.
Benz, C. (2015). “Chip‐Based Free‐Flow Electrophoresis with Integrated Nanospray Mass‐Spectrometry”. Angewandte Chemie International Edition 54(9), 2766-2770.