EM Data Processing and Interpretation

Electron Microscopy (EM) is a powerful magnification tool utilizing focused beams of electrons to obtain topographical, morphological and compositional information for a large variety of biological samples, which makes it invaluable in many science and industry applications. Advances in sample preparation methods and development of EM instrumentation have allowed electron micrographs to be acquired with ever-increasing resolution. However, the processing and analysis of EM data remains a difficult step, which relies heavily on expertise and the use of proper software tools.

The EM platform at Creative Biostructure covers a wide spectrum of techniques for sample preparation, imaging, as well as image analysis in 2D and 3D. Equipped with high-end image analysis workstations, our major focus is the study of large macromolecules, but we can also implement the ultrastructural analysis of microorganisms, nanoparticles, and cellular/tissue specimens. We have extensive experiences in automated and manual image and data analysis methods and a good master of software tools for statistical analysis and mathematical modelling.

Typical types of our services include:

• TEM/SEM image enhancement, segmentation, signal to noise ratio optimization
• Nanoparticle morphology, circularity, homogeneity and size distribution
• Electron tomography of cellular structures with subnanometer resolution
• Automatic particle picking and multi-class classification
• 2D class averaging and 3D reconstruction
• Localization and identification of specific macromolecules in tomographic volumes
• Fitting of individual structures into cryo-EM maps of macromolecular complexes
• Hybrid approaches combined with available structural information
• Customizable analysis based on requests

Figure 1. EM data processing and analysis

Creative Biostructure uses various leading software packages for advanced EM data evaluation and analysis. Our staff can also help you define optimal experimental conditions and sample preparation methods for your biological samples, ensuring the collection of highest-quality data for interpretation. We promise to work closely with our customers to provide tailored EM solutions for a wide spectrum of biological samples.

Please feel free to contact us for a detailed quote.

Ordering Process

Reference

1. Carazo JM, et al. (2015) “Three-dimensional reconstruction methods in Single Particle Analysis from transmission electron microscopy data”. Arch Biochem Biophys 581:39-48.