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Combinatorial Assembly Libraries

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Creative Biostructure has rich professional experience in custom combinatorial assembly libraries services for directed protein evolution. The combinatorial assembly library offers a great platform for protein function and active center studies.

Combinatorial assembly library is a method to build customized variants of DNA parts (e.g. promoters, ribosomal binding sites, UTRs, open reading frames, signal peptides terminators, etc.) that combine multiple genetic elements in predefined arrangements. The final construct is chemically synthesized, with sequence junctions and frame assembled by seamless cloning technique according to your specific demands. Creative Biostructure is able to complete DNA sequence customization to enhance protein expression levels. We have strong expertise in synthesizing combinatorial assembly libraries of any size and complexity at the lower price.

Creative Biostructure can provide world-leading custom combinatorial assembly libraries services, including but not limited to:

  • Combinaion of different genetic components for metabolic engineering, such as enzymes, sensors, feedback devices (e.g. transcriptional, allosteric, or degradation regulators).
  • Combination of different regulatory elements (promoters, repressors, terminators), coding sequences (ORFs) and/or tags useful for monitoring gene expression (GFP, luciferase) or purifying protein (FLAG, GST, HIS).
  • Creation of modular DNA sequences of any length.
  • Design of novel genetic components and/or use existing interchangeable modules.

Creative Biostructure ensures that all conceivable combinations can be generated to identify the most valuable combination under a variety of expression cassettes. The size of the combinatorial assembly library and the strategy for combining components are tailored to your project needs. Our custom services have a number of advantages:

  • Tested for accuracy–All combinatorial assembly libraries are delivered with 100% sequence accuracy.
  • Cost-effective–All libraries have the potential to be used multiple times.
  • Our services can meet your requirements for projects of any size.
  • A guaranteed report and quality assurance certificate for your project can be delivered.

Applications for combinatorial assembly libraries provided by Creative Biostructure, including:

  • Screening numerous unique clones that express different combinations of modules;
  • Selecting or screening for synthetic gene circuits;
  • High-throughput synthesis of variant constructs for genome editing;
  • Optimizing protein function through directed evolution;
  • Generating recombinant protein or chimeric antibody libraries;
  • Determining the optimal combinations (promoter, ribosome binding site, terminator, and other regulatory elements) for the construct/expression system;
  • Other potential applications.

Creative Biostructure provides various protein engineering and analysis services, including protein evolution and mutagenesis libraries construction. Please feel free to contact us to discuss your project!

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References:
M. Krier, et al. (2005). Design of Small-Sized Libraries by Combinatorial Assembly of Linkers and Functional Groups to a Given Scaffold: Application to the Structure-Based Optimization of a Phosphodiesterase 4 Inhibitor. J. Med. Chem., 48: 3816-3822.
V. E. Wall, et al. (2014). Combinatorial assembly of clone libraries using site-specific recombination. Methods Mol. Biol., 1116: 193-208. doi: 10.1007/978-1-62703-764-8_14.


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