In the field of liposome research & development, Creative Biostructure has accumulated rich experience through hundreds of successful scientific projects in recent years. Integrated with other advanced platforms, Creative Biostructure is able to provide the most comprehensive products and services based on Mempro™ Liposome platform.
Liposome stability during storage/processing and liposome stability in human body are two major concerns in liposome design and application. The former is crucial to liposomes in food applications, while the latter is extremely vital to liposomes in pharmaceutical applications.
Figure 1. Liposome encapsulated DHA was exposed to various pHs from 1 to 7.4 for 10 min, one hour, and two hours. Dynamic light scattering (DLS) was performed at every hour and at various pHs. All diameters are presented in nanometers. Though the size of liposome increased to 156.4 ± 0.3 nm at pH 1, the size of liposome was still below 200 nm, indicating the liposomal DHA is ideal for the uptake and transfer in stomach. (C. G. Skibinski, et al., 2016)
Liposome behaviour and characteristics are highly dependent on its composition, making it very difficult to predict the structural and functional stability of liposome system under different stresses such as temperature, pH and the presence of degradative substances. Therefore, functional liposomes must be proved to have adequate stability in considerations of both chemical and physical changes.
Defects in lattice structure can lead to liposome leakage or liposome fusion, and analysis techniques based on size changes are unable to well differ actual fusion from aggregation, making analysis methods based on fluorescence changes such as FRET and epifluorescence microscopy quite useful to measure the physical stability of liposome. Freezing, spray-drying and freeze-drying are considered in order to guarantee the long term stability of liposome. Furthermore, to measure the ability of liposomes to keep the encapsulated agents for as long as required is one of the most important stability measurements.
Degradation of natural phospholipids occurs mainly in two ways, one is the peroxidation of double bonds present in acyl chains, the other one is the hydrolysis of ester bonds linking fatty acids to the glycerol moiety. Usage of high quality lipids and correct preparation and storage are effective ways to minimize the degradation of liposome through peroxidation. To reduce the oxidation rate, liposomes must be keep from light and oxygen. There are a lot of methods can be used to monitor the degradation of lipids. For example, thin layer chromatography (TLC) can be applied to measure the purity and concentration of the lipids.
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Besides stability analysis, a wide variety of liposome analysis services based on our advanced Mempro™ Liposome Technology are also available in the related sections. Please feel free to contact us for a detailed quote.
C. G. Skibinski, et al. (2016). A novel biologically active acid stable liposomal formulation of docosahexaenoic acid in human breast cancer cell lines. Chemico-Biological Interactions, 256: 1-8.
M. Marsanasco, et al. (2015). Bioactive constituents in liposomes incorporated in orange juice as new functional food: thermal stability, rheological and organoleptic properties. J. Food Sci. Technol., 52(12): 7828–7838.
J. F. Boelter and A Brandelli. (2016). Innovative bionanocomposite films of edible proteins containing liposome-encapsulated nisin and halloysite nanoclay. Colloids and Surfaces B: Biointerfaces, 145: 740-747.