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The Creative Biostructure's Protein Crystallography Starter Kit (PCSK) can help you to mount and collect X-ray data from your crystals at both room and cryo temperatures. This starter kit is a perfect training tool for new school students and technicians.
|Kit Components||Additional Required Material not Included in the Kit|
|24-well Super Clear plates||Pipettes with volumes of 1-500 µl|
|1 syringe with 5 ml silicone grease||Accompanying pipette tips|
|100 cover slides||Deionized water|
|1 microscope slide||Tweezers|
|20 ml of 20% (m/v) NaCl solution||Temperature-controlled room (20–22 °C)|
|3 ml each of 250 mM Na-acetate buffer pH 4.0~5.2||Microscope|
|200 µl lysozyme solution (20 mg/ml) in water|
8 ml precipitant solution for batch-crystallization
(30% (m/v) PEG 5000-MME, 1 M NaCl, 50 mM Na-acetate pH 4.4)
|A user handbook|
This PCSK can introduce students and technicians to the field of protein crystallization technology. It contains all the materials you need to start growing a great looking crystal – It’s a real highlight in biochemistry courses.
There are two different experiments can be carried out by using this PCSK:
1. Growing crystals using the hanging drop method, demonstrating the dependence of crystal formation and crystal growth.
2. Growing crystals within minutes using the batch crystallization method.
The protein crystallization starter kit also contains two crystallization plates: cover slides and a microscope slide, a syringe pre-filled with sealing grease, all necessary buffer and salt stock solutions and a small tube containing pre-filtered lysozyme solution.