Creative Biostructure has strong expertise in generating and selecting protein variants to obtain desired protein function in protein structural biology and functionality studies. It is well known that producing a large collection of protein variants is often time-consuming and labor-intensive. Based on our protein evolution and engineering platform, our professional scientists can perform high-throughput gene synthesis, mutagenesis and protein production to help speed up your project by offering high-throughput protein variants production service which can deliver up to 1,000 protein variants within 30 business days.
Figure 1. High-throughput screening of dynamic protein polymers SELPs. (Adv. Funct. Mater, 2014)
Creative Biostructure can accomplish custom high-throughput protein variants service through the evaluation of expression yield from multiple gene constructs, vectors, growth conditions and expression systems to determine which combinations can meet the needs of your project. Our service includes gene synthesis, mutagenesis, subcloning, pilot scale protein expression in E. coli system, and protein purification. We have different strategies for high-throughput cloning and expression in cell-based expression systems, such as bacterial, yeast, mammalian and baculovirus-infected insect cells. Additionally, bacterial projects can be processed in multiples (or subsets) of 96, while baculovirus projects in multiples of 24 (up to 48 at a time). The average length of expression testing is 3-5 weeks for bacteria, 6-8 weeks for baculovirus. The 24-well block format is also being applied for utility with mammalian cells. In addition, we can perform design of oligos and additional sequences to obtain optimal efficiency in PCR production. PCR reactions, LIC reactions, transformations, plating and plasmid minipreps are all carried out in multi-well formats (24- or 96-well).
Our customized high-throughput protein variants service is well suited structural and functional genomics, protein microarrays and protein variants screening strategies. Our high-throughput protein variants service has lots of advantages, including but not limited to:
Our high-throughput protein variants package contains:
Creative Biostructure’s custom protein engineering services can provide you a broad range of applications. Creative Biostructure also has deep expertise and decades of experience in provides Membrane Protein Platform and Phage Display Platform for your specific projects. Please feel free to contact us for a detailed quote.
References:P. Savitsky, et al. (2010). High-throughput production of human proteins for crystallization: The SGC experience. J. Struct. Biol., 172(1): 3-13.Q. Wang, et al. (2014). High Throughput Screening of Dynamic Silk-Elastin-Like Protein Biomaterials. Adv. Funct. Mater., 24(27). DOI: 10.1002/adfm.201304106.V. Jäger, et al. (2013). High level transient production of recombinant antibodies and antibody fusion proteins in HEK293 cells. BMC Biotechnol., 13:52. DOI: 10.1186/1472-6750-13-52.