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Product Summary
Source
E.coli
Product Overview
Recombinant human membrane scaffold protein 1 D1 delta H5 mutant was expressed in E.coli and purified by Ni-NTA.
Purity
>90%
Species
Human
Tag
His
Molecular Mass
21.68kDa
Storage
Stored lyophilized powder at -20°C. The reconstituted solution should be stored at 2-8°C and should be used up in several days.
Background
Nanodiscs are a new class of model membranes that are being used to solubilize and study a range of integral membrane proteins and membrane-associated proteins. The Nanodisc bilayer is bounded by a membrane scaffold protein coat that confers enhanced stability and a narrow particle size distribution. The nanodisc assembles from a mixture of full length membrane protein in detergent, phospholipid micelles and membrane scaffold protein upon removal of the detergent.
Reconstitution
Adding double distilled watwe to prepare a stock solution of 4mg/mL. This stock solution can be diluted further as required by the differnet application protocols.
Scaffold Diameter
7-8 nm
Formulation
Lyophilized from 20mM Tris pH 7.4, 100 mM NaCl, 0.5 mM EDTA.