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Product Summary
Source
E.coli
Product Overview
Recombinant human membrane scaffold protein 2N2 was expressed in E.coli and purified by Ni-NTA.
Purity
>90%
Species
Human
Tag
His
Molecular Mass
45.67kDa
Storage
Stored lyophilized powder at -20°C. The reconstituted solution should be stored at 2-8°C and should be used up in several days.
Background
Nanodiscs are a new class of model membranes that are being used to solubilize and study a range of integral membrane proteins and membrane-associated proteins. The Nanodisc bilayer is bounded by a membrane scaffold protein coat that confers enhanced stability and a narrow particle size distribution. The nanodisc assembles from a mixture of full length membrane protein in detergent, phospholipid micelles and membrane scaffold protein upon removal of the detergent.
Reconstitution
Adding double distilled watwe to prepare a stock solution of 4mg/mL. This stock solution can be diluted further as required by the differnet application protocols.
Scaffold Diameter
17 nm
Formulation
Lyophilized from 20mM Tris pH 7.4, 100 mM NaCl, 0.5 mM EDTA.