List of Restriction Enzyme Sites

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Browse restriction enzyme based on alphabetical listing:

Enzyme Name	Prototype	Sequence	Cut Site	Overhang
AaaI	XmaIII	CGGCCG	C^GGCCG GCCGG^C	5' - CCGG
AagI	ClaI	ATCGAT	AT^CGAT TAGC^TA	5' - GC
AarI		CACCTGC	CACCTGCNNNN^NNNN GTGGACGNNNNNNNN^	5' - NNNN
AasI	DrdI	GACNNNNNNGTC	GACNNNN^NNGTC CTGNN^NNNNCAG	NN - 3'
AatI	StuI	AGGCCT	AGG^CCT TCC^GGA	Blunt
AatII		GACGTC	GACGT^C C^TGCAG	ACGT - 3'
AauI	Bsp1407I	TGTACA	T^GTACA ACATG^T	5' - CATG
AbaI	BclI	TGATCA	T^GATCA ACTAG^T	5' - CTAG
AbeI	BbvCI	CCTCAGC	CC^TCAGC GGAGT^CG	5' - AGT
AbrI	XhoI	CTCGAG	C^TCGAG GAGCT^C	5' - AGCT
AccI		GTMKAC	GT^MKAC CAMK^TG	5' - MK
AccII	FnuDII	CGCG	CG^CG GC^GC	Blunt
AccIII	BspMII	TCCGGA	T^CCGGA AGGCC^T	5' - GGCC
Acc16I	MstI	TGCGCA	TGC^GCA ACG^CGT	Blunt
Acc36I	BspMI	ACCTGC	ACCTGCNNNN^NNNN TGGACGNNNNNNNN^	5' - NNNN
Acc65I	KpnI	GGTACC	G^GTACC CCATG^G	5' - CATG
Acc113I	ScaI	AGTACT	AGT^ACT TCA^TGA	Blunt
AccB1I	HgiCI	GGYRCC	G^GYRCC CCYRG^G	5' - CYRG
AccB2I	HaeII	RGCGCY	RGCGC^Y R^CGCGY	GCGC - 3'
AccB7I	PflMI	CCANNNNNTGG	CCANNNN^NTGG GGTN^NNNNACC	NNN - 3'
AccBSI	BsrBI	CCGCTC	CCG^CTC GGC^GAG	Blunt
AccEBI	BamHI	GGATCC	G^GATCC CCTAG^G	5' - CTAG
AceI	TseI	GCWGC	G^CWGC CGWC^G	5' - GWC
AceII	NheI	GCTAGC	GCTAG^C C^GATCG	CTAG - 3'
AceIII		CAGCTC	CAGCTCNNNNNNN^NNNN GTCGAGNNNNNNNNNNN^	5' - NNNN
AciI		CCGC	C^CGC GGC^G	5' - GC
AclI		AACGTT	AA^CGTT TTGC^AA	5' - GC
AclNI	SpeI	ACTAGT	A^CTAGT TGATC^A	5' - GATC
AclWI	BinI	GGATC	GGATCNNNN^N CCTAGNNNNN^	5' - N
AcpI	AsuII	TTCGAA	TT^CGAA AAGC^TT	5' - GC
AcpII	PflMI	CCANNNNNTGG	CCANNNN^NTGG GGTN^NNNNACC	NNN - 3'
AcrII	BstEII	GGTNACC	G^GTNACC CCANTG^G	5' - CANTG
AcsI	ApoI	RAATTY	R^AATTY RTTAA^Y	5' - TTAA
AcuI	Eco57I	CTGAAG	CTGAAGNNNNNNNNNNNNNNNN^ GACTTCNNNNNNNNNNNNNN^NN	NN - 3'
AcvI	PmaCI	CACGTG	CAC^GTG GTG^CAC	Blunt
AcyI		GRCGYC	GR^CGYC CRGC^YG	5' - GC
AdeI	DraIII	CACNNNGTG	CACNNN^GTG GTG^NNNCAC	NNN - 3'
AeuI	EcoRII	CCWGG	CC^WGG GGW^CC	5' - W
AfaI	RsaI	GTAC	GT^AC CA^TG	Blunt
Afa22MI	PvuI	CGATCG	CGAT^CG GC^TAGC	AT - 3'
Afa16RI	PvuI	CGATCG	CGAT^CG GC^TAGC	AT - 3'
AfeI	Eco47III	AGCGCT	AGC^GCT TCG^CGA	Blunt
AflI	AvaII	GGWCC	G^GWCC CCWG^G	5' - CWG
AflII		CTTAAG	C^TTAAG GAATT^C	5' - AATT
AflIII		ACRYGT	A^CRYGT TGRYC^A	5' - GRYC
AgeI		ACCGGT	A^CCGGT TGGCC^A	5' - GGCC
AglI	EcoRII	CCWGG	CC^WGG GGW^CC	5' - W
AhaI	CauII	CCSGG	CC^SGG GGS^CC	5' - S
AhaII	AcyI	GRCGYC	GR^CGYC CRGC^YG	5' - GC
AhaIII		TTTAAA	TTT^AAA AAA^TTT	Blunt
AhaB8I	KpnI	GGTACC	G^GTACC CCATG^G	5' - CATG
AhdI	Eam1105I	GACNNNNNGTC	GACNNN^NNGTC CTGNN^NNNCAG	N - 3'
AhlI	SpeI	ACTAGT	A^CTAGT TGATC^A	5' - GATC
AhyI	SmaI	CCCGGG	C^CCGGG GGGCC^C	5' - GGCC
AitI	Eco47III	AGCGCT	AGC^GCT TCG^CGA	Blunt
AjnI	EcoRII	CCWGG	^CCWGG GGWCC^	5' - GGWCC
AjoI	PstI	CTGCAG	CTGCA^G G^ACGTC	TGCA - 3'
AleI	OliI	CACNNNNGTG	CACNN^NNGTG GTGNN^NNCAC	Blunt
AlfI		GCANNNNNNTGC	GCANNNNNNTGCNNNNNNNNNNNN^ CGTNNNNNNACGNNNNNNNNNN^NN	NN - 3'
AliI	BamHI	GGATCC	G^GATCC CCTAG^G	5' - CTAG
AliAJI	PstI	CTGCAG	CTGCA^G G^ACGTC	TGCA - 3'
AloI		GAACNNNNNNTCC	GAACNNNNNNTCCNNNNNNNNNNNN^ CTTGNNNNNNAGGNNNNNNN^NNNNN	NNNNN - 3'
AluI		AGCT	AG^CT TC^GA	Blunt
AlwI	BinI	GGATC	GGATCNNNN^N CCTAGNNNNN^	5' - N
Alw21I	HgiAI	GWGCWC	GWGCW^C C^WCGWG	WGCW - 3'
Alw26I	BsmAI	GTCTC	GTCTCN^NNNN CAGAGNNNNN^	5' - NNNN
Alw44I	ApaLI	GTGCAC	G^TGCAC CACGT^G	5' - ACGT
AlwNI		CAGNNNCTG	CAGNNN^CTG GTC^NNNGAC	NNN - 3'
AlwXI	BbvI	GCAGC	GCAGCNNNNNNNN^NNNN CGTCGNNNNNNNNNNNN^	5' - NNNN
Ama87I	AvaI	CYCGRG	C^YCGRG GYGCR^C	5' - YGCR
AocI	SauI	CCTNAGG	CC^TNAGG GGANT^CC	5' - ANT
AocII	SduI	GDGCHC	GDGCH^C C^DCGHG	DGCH - 3'
AorI	EcoRII	CCWGG	CC^WGG GGW^CC	5' - W
Aor13HI	BspMII	TCCGGA	T^CCGGA AGGCC^T	5' - GGCC
Aor51HI	Eco47III	AGCGCT	AGC^GCT TCG^CGA	Blunt
AosI	MstI	TGCGCA	TGC^GCA ACG^CGT	Blunt
AosII	AcyI	GRCGYC	GR^CGYC CRGC^YG	5' - GC
ApaI		GGGCCC	GGGCC^C C^CCGGG	GGCC - 3'
ApaBI		GCANNNNNTGC	GCANNNNN^TGC CGT^NNNNNACG	NNNNN - 3'
ApaCI	BamHI	GGATCC	G^GATCC CCTAG^G	5' - CTAG
ApaLI		GTGCAC	G^TGCAC CACGT^G	5' - ACGT
ApaORI	EcoRII	CCWGG	CC^WGG GGW^CC	5' - W
ApeKI	TseI	GCWGC	G^CWGC CGWC^G	5' - GWC
ApiI	PstI	CTGCAG	CTGCA^G G^ACGTC	TGCA - 3'
ApoI		RAATTY	R^AATTY RTTAA^Y	5' - TTAA
ApyI	EcoRII	CCWGG	CC^WGG GGW^CC	5' - W
AquI	AvaI	CYCGRG	C^YCGRG GYGCR^C	5' - YGCR
AscI		GGCGCGCC	GG^CGCGCC CCGCGC^GG	5' - GCGC
AseI	VspI	ATTAAT	AT^TAAT TAAT^TA	5' - AT
AseII	CauII	CCSGG	CC^SGG GGS^CC	5' - S
AsiI	BamHI	GGATCC	G^GATCC CCTAG^G	5' - CTAG
AsiAI	AgeI	ACCGGT	A^CCGGT TGGCC^A	5' - GGCC
AsiSI	SgfI	GCGATCGC	GCGAT^CGC CGC^TAGCG	AT - 3'
AsnI	VspI	ATTAAT	AT^TAAT TAAT^TA	5' - AT
AspI	Tth111I	GACNNNGTC	GACN^NNGTC CTGNN^NCAG	5' - N
Asp700I	XmnI	GAANNNNTTC	GAANN^NNTTC CTTNN^NNAAG	Blunt
Asp713I	PstI	CTGCAG	CTGCA^G G^ACGTC	TGCA - 3'
Asp718I	KpnI	GGTACC	G^GTACC CCATG^G	5' - CATG
Asp745I	AvaII	GGWCC	G^GWCC CCWG^G	5' - CWG
AspAI	BstEII	GGTNACC	G^GTNACC CCANTG^G	5' - CANTG
AspA2I	AvrII	CCTAGG	C^CTAGG GGATC^C	5' - GATC
AspEI	Eam1105I	GACNNNNNGTC	GACNNN^NNGTC CTGNN^NNNCAG	N - 3'
AspHI	HgiAI	GWGCWC	GWGCW^C C^WCGWG	WGCW - 3'
Asp10HI	AsuII	TTCGAA	TT^CGAA AAGC^TT	5' - GC
Asp10HII	PflMI	CCANNNNNTGG	CCANNNN^NTGG GGTN^NNNNACC	NNN - 3'
Asp26HI	BsmI	GAATGC	GAATGCN^ CTTAC^GN	CN - 3'
Asp27HI	BsmI	GAATGC	GAATGCN^ CTTAC^GN	CN - 3'
Asp35HI	BsmI	GAATGC	GAATGCN^ CTTAC^GN	CN - 3'
Asp36HI	BsmI	GAATGC	GAATGCN^ CTTAC^GN	CN - 3'
Asp40HI	BsmI	GAATGC	GAATGCN^ CTTAC^GN	CN - 3'
Asp50HI	BsmI	GAATGC	GAATGCN^ CTTAC^GN	CN - 3'
AspLEI	HhaI	GCGC	GCG^C C^GCG	CG - 3'
AspMI	StuI	AGGCCT	AGG^CCT TCC^GGA	Blunt
AspMDI	MboI	GATC	^GATC CTAG^	5' - CTAG
AspNI	NlaIV	GGNNCC	GGN^NCC CCN^NGG	Blunt
AspS9I	AsuI	GGNCC	G^GNCC CCNG^G	5' - CNG
AssI	ScaI	AGTACT	AGT^ACT TCA^TGA	Blunt
AstWI	AcyI	GRCGYC	GR^CGYC CRGC^YG	5' - GC
AsuI		GGNCC	G^GNCC CCNG^G	5' - CNG
AsuII		TTCGAA	TT^CGAA AAGC^TT	5' - GC
AsuIII	AcyI	GRCGYC	GR^CGYC CRGC^YG	5' - GC
AsuC2I	CauII	CCSGG	CC^SGG GGS^CC	5' - S
AsuHPI	HphI	GGTGA	GGTGANNNNNNNN^ CCACTNNNNNNN^N	N - 3'
AsuNHI	NheI	GCTAGC	G^CTAGC CGATC^G	5' - GATC
AtsI	Tth111I	GACNNNGTC	GACN^NNGTC CTGNN^NCAG	5' - N
AvaI		CYCGRG	C^YCGRG GYGCR^C	5' - YGCR
AvaII		GGWCC	G^GWCC CCWG^G	5' - CWG
AvcI	AsuI	GGNCC	G^GNCC CCNG^G	5' - CNG
AviII	MstI	TGCGCA	TGC^GCA ACG^CGT	Blunt
AvrII		CCTAGG	C^CTAGG GGATC^C	5' - GATC
AvrBII	AvrII	CCTAGG	C^CTAGG GGATC^C	5' - GATC
AxyI	SauI	CCTNAGG	CC^TNAGG GGANT^CC	5' - ANT
AanI	PsiI	TTATAA	TTA^TAA AAT^ATT	Blunt
AbsI		CCTCGAGG	CC^TCGAGG GGAGCT^CC	5' - AGCT
AcoI	CfrI	YGGCCR	Y^GGCCR YCCGG^R	5' - CCGG
AfiI		GCNNNNNNNGG	GCNNNNN^NNGG CGNN^NNNNNCC	NNN - 3'
AjiI	BtrI	CACGTC	CAC^GTC GTG^CAG	Blunt
AluBI	AluI	AGCT	AG^CT TC^GA	Blunt
AsiGI	AgeI	ACCGGT	A^CCGGT TGGCC^A	5' - GGCC
Bac36I	AsuI	GGNCC	G^GNCC CCNG^G	5' - CNG
BaeI		ACNNNNGTAYC	ACNNNNGTAYCNNNNNNNNNNNN^ TGNNNNCATYGNNNNNNN^NNNNN	NNNNN - 3'
BalI		TGGCCA	TGG^CCA ACC^GGT	Blunt
Bal228I	AsuI	GGNCC	G^GNCC CCNG^G	5' - CNG
BamHI		GGATCC	G^GATCC CCTAG^G	5' - CTAG
BamNxI	AvaII	GGWCC	G^GWCC CCWG^G	5' - CWG
BanI	HgiCI	GGYRCC	G^GYRCC CCYRG^G	5' - CYRG
BanII	HgiJII	GRGCYC	GRGCY^C C^RCGYG	RGCY - 3'
BanIII	ClaI	ATCGAT	AT^CGAT TAGC^TA	5' - GC
BanAI	HaeIII	GGCC	GG^CC CC^GG	Blunt
BasI	PflMI	CCANNNNNTGG	CCANNNN^NTGG GGTN^NNNNACC	NNN - 3'
BauI	BsiI	CACGAG	C^ACGAG GTGCT^C	5' - TGCT
BavI	PvuII	CAGCTG	CAG^CTG GTC^GAC	Blunt
BavAI	PvuII	CAGCTG	CAG^CTG GTC^GAC	Blunt
BavAII	AsuI	GGNCC	G^GNCC CCNG^G	5' - CNG
BavBI	PvuII	CAGCTG	CAG^CTG GTC^GAC	Blunt
BavBII	AsuI	GGNCC	G^GNCC CCNG^G	5' - CNG
BavCI	ClaI	ATCGAT	AT^CGAT TAGC^TA	5' - GC
BbeI	NarI	GGCGCC	GGCGC^C C^CGCGG	GCGC - 3'
BbiII	AcyI	GRCGYC	GR^CGYC CRGC^YG	5' - GC
Bbi24I	MluI	ACGCGT	A^CGCGT TGCGC^A	5' - GCGC
BbrI	HindIII	AAGCTT	A^AGCTT TTCGA^A	5' - TCGA
Bbr7I	BbvII	GAAGAC	GAAGACNNNNNNN^NNNN CTTCTGNNNNNNNNNNN^	5' - NNNN
BbrPI	PmaCI	CACGTG	CAC^GTG GTG^CAC	Blunt
BbsI	BbvII	GAAGAC	GAAGACNN^NNNN CTTCTGNNNNNN^	5' - NNNN
BbuI	SphI	GCATGC	GCATG^C C^GTACG	CATG - 3'
BbvI		GCAGC	GCAGCNNNNNNNN^NNNN CGTCGNNNNNNNNNNNN^	5' - NNNN
BbvII		GAAGAC	GAAGACNN^NNNN CTTCTGNNNNNN^	5' - NNNN
Bbv12I	HgiAI	GWGCWC	GWGCW^C C^WCGWG	WGCW - 3'
Bbv16II	BbvII	GAAGAC	GAAGACNN^NNNN CTTCTGNNNNNN^	5' - NNNN
BbvAI	XmnI	GAANNNNTTC	GAANN^NNTTC CTTNN^NNAAG	Blunt
BbvAII	ClaI	ATCGAT	AT^CGAT TAGC^TA	5' - GC
BbvAIII	BspMII	TCCGGA	T^CCGGA AGGCC^T	5' - GGCC
BbvBI	HgiCI	GGYRCC	G^GYRCC CCYRG^G	5' - CYRG
BbvCI		CCTCAGC	CC^TCAGC GGAGT^CG	5' - AGT
Bca77I	BetI	WCCGGW	W^CCGGW WGGCC^W	5' - GGCC
BccI		CCATC	CCATCNNNN^N GGTAGNNNNN^	5' - N
Bce4I	MwoI	GCNNNNNNNGC	GCNNNNN^NNGC CGNN^NNNNNCG	NNN - 3'
Bce22I	AsuI	GGNCC	G^GNCC CCNG^G	5' - CNG
Bce83I		CTTGAG	CTTGAGNNNNNNNNNNNNNNNN^ GAACTCNNNNNNNNNNNNNN^NN	NN - 3'
Bce243I	MboI	GATC	^GATC CTAG^	5' - CTAG
Bce751I	BamHI	GGATCC	G^GATCC CCTAG^G	5' - CTAG
BceAI	BcefI	ACGGC	ACGGCNNNNNNNNNNNN^NN TGCCGNNNNNNNNNNNNNN^	5' - NN
BceBI	FnuDII	CGCG	CG^CG GC^GC	Blunt
BceCI	MwoI	GCNNNNNNNGC	GCNNNNN^NNGC CGNN^NNNNNCG	NNN - 3'
BcefI		ACGGC	ACGGCNNNNNNNNNNNN^N TGCCGNNNNNNNNNNNNN^	5' - N
BcgI		CGANNNNNNTGC	CGANNNNNNTGCNNNNNNNNNNNN^ GCTNNNNNNACGNNNNNNNNNN^NN	NN - 3'
Bci29I	ClaI	ATCGAT	AT^CGAT TAGC^TA	5' - GC
BciBI	ClaI	ATCGAT	AT^CGAT TAGC^TA	5' - GC
BciBII	EcoRII	CCWGG	CC^WGG GGW^CC	5' - W
BciVI		GTATCC	GTATCCNNNNNN^ CATAGGNNNNN^N	N - 3'
BclI		TGATCA	T^GATCA ACTAG^T	5' - CTAG
BcmI	ClaI	ATCGAT	AT^CGAT TAGC^TA	5' - GC
BcnI	CauII	CCSGG	CC^SGG GGS^CC	5' - S
BcoI	AvaI	CYCGRG	C^YCGRG GYGCR^C	5' - YGCR
Bco5I	Ksp632I	CTCTTC	CTCTTCN^NNN GAGAAGNNNN^	5' - NNN
Bco27I	HpaII	CCGG	C^CGG GGC^C	5' - GC
Bco116I	Ksp632I	CTCTTC	CTCTTCN^NNN GAGAAGNNNN^	5' - NNN
Bco118I	Cfr10I	RCCGGY	R^CCGGY RGGCC^Y	5' - GGCC
BcoAI	PmaCI	CACGTG	CAC^GTG GTG^CAC	Blunt
BcoKI	Ksp632I	CTCTTC	CTCTTCN^NNN GAGAAGNNNN^	5' - NNN
BcuI	SpeI	ACTAGT	A^CTAGT TGATC^A	5' - GATC
BcuAI	AvaII	GGWCC	G^GWCC CCWG^G	5' - CWG
BdiI	ClaI	ATCGAT	AT^CGAT TAGC^TA	5' - GC
BdiSI	SfeI	CTRYAG	C^TRYAG GARYT^C	5' - ARYT
BecAII	HaeIII	GGCC	GG^CC CC^GG	Blunt
BepI	FnuDII	CGCG	CG^CG GC^GC	Blunt
BetI		WCCGGW	W^CCGGW WGGCC^W	5' - GGCC
BfaI	MaeI	CTAG	C^TAG GAT^C	5' - AT
BfiI		ACTGGG	ACTGGGNNNNN^ TGACCCNNNN^N	N - 3'
Bfi57I	MboI	GATC	^GATC CTAG^	5' - CTAG
Bfi89I	CfrI	YGGCCR	Y^GGCCR YCCGG^R	5' - CCGG
BflI	BsiYI	CCNNNNNNNGG	CCNNNNN^NNGG GGNN^NNNNNCC	NNN - 3'
BfmI	SfeI	CTRYAG	C^TRYAG GARYT^C	5' - ARYT
BfrI	AflII	CTTAAG	C^TTAAG GAATT^C	5' - AATT
BfrBI	AvaIII	ATGCAT	ATG^CAT TAC^GTA	Blunt
BfuI	BciVI	GTATCC	GTATCCNNNNNN^ CATAGGNNNNN^N	N - 3'
BfuAI	BspMI	ACCTGC	ACCTGCNNNN^NNNN TGGACGNNNNNNNN^	5' - NNNN
BfuCI	MboI	GATC	^GATC CTAG^	5' - CTAG
BglI		GCCNNNNNGGC	GCCNNNN^NGGC CGGN^NNNNCCG	NNN - 3'
BglII		AGATCT	A^GATCT TCTAG^A	5' - CTAG
BimI	AsuII	TTCGAA	TT^CGAA AAGC^TT	5' - GC
Bim19I	AsuII	TTCGAA	TT^CGAA AAGC^TT	5' - GC
Bim19II	HaeIII	GGCC	GG^CC CC^GG	Blunt
BinI		GGATC	GGATCNNNN^N CCTAGNNNNN^	5' - N
BlfI	BspMII	TCCGGA	T^CCGGA AGGCC^T	5' - GGCC
Bli41I	ClaI	ATCGAT	AT^CGAT TAGC^TA	5' - GC
Bli86I	ClaI	ATCGAT	AT^CGAT TAGC^TA	5' - GC
Bli736I	Eco31I	GGTCTC	GGTCTCN^NNNN CCAGAGNNNNN^	5' - NNNN
BliAI	ClaI	ATCGAT	AT^CGAT TAGC^TA	5' - GC
BliHKI	SauI	CCTNAGG	CC^TNAGG GGANT^CC	5' - ANT
BliRI	ClaI	ATCGAT	AT^CGAT TAGC^TA	5' - GC
BlnI	AvrII	CCTAGG	C^CTAGG GGATC^C	5' - GATC
BloHI	XhoII	RGATCY	R^GATCY RCTAG^Y	5' - CTAG
BloHII	PstI	CTGCAG	CTGCA^G G^ACGTC	TGCA - 3'
BlpI	EspI	GCTNAGC	GC^TNAGC CGANT^CG	5' - ANT
BluI	XhoI	CTCGAG	C^TCGAG GAGCT^C	5' - AGCT
Bme12I	MboI	GATC	^GATC CTAG^	5' - CTAG
Bme18I	AvaII	GGWCC	G^GWCC CCWG^G	5' - CWG
Bme142I	HaeII	RGCGCY	RGC^GCY RCG^CGY	Blunt
Bme216I	AvaII	GGWCC	G^GWCC CCWG^G	5' - CWG
Bme361I	HaeIII	GGCC	GG^CC CC^GG	Blunt
Bme585I	FauI	CCCGC	CCCGCNNNN^NN GGGCGNNNNNN^	5' - NN
Bme1390I	ScrFI	CCNGG	CC^NGG GGN^CC	5' - N
Bme1580I	BseSI	GKGCMC	GKGCM^C C^KCGMG	KGCM - 3'
BmgBI	BtrI	CACGTC	CAC^GTC GTG^CAG	Blunt
BmrI	BfiI	ACTGGG	ACTGGGNNNNN^ TGACCCNNNN^N	N - 3'
BmtI	NheI	GCTAGC	GCTAG^C C^GATCG	CTAG - 3'
BmyI	SduI	GDGCHC	GDGCH^C C^DCGHG	DGCH - 3'
BnaI	BamHI	GGATCC	G^GATCC CCTAG^G	5' - CTAG
BoxI	PshAI	GACNNNNGTC	GACNN^NNGTC CTGNN^NNCAG	Blunt
BpcI	SfeI	CTRYAG	C^TRYAG GARYT^C	5' - ARYT
BpiI	BbvII	GAAGAC	GAAGACNN^NNNN CTTCTGNNNNNN^	5' - NNNN
BplI		GAGNNNNNCTC	GAGNNNNNCTCNNNNNNNNNNNNN^ CTCNNNNNGAGNNNNNNNN^NNNNN	NNNNN - 3'
BpmI	GsuI	CTGGAG	CTGGAGNNNNNNNNNNNNNNNN^ GACCTCNNNNNNNNNNNNNN^NN	NN - 3'
BpoAI	VspI	ATTAAT	AT^TAAT TAAT^TA	5' - AT
BptI	EcoRII	CCWGG	CC^WGG GGW^CC	5' - W
BpuI	HgiJII	GRGCYC	GRGCY^C C^RCGYG	RGCY - 3'
Bpu10I		CCTNAGC	CC^TNAGC GGANT^CG	5' - ANT
Bpu14I	AsuII	TTCGAA	TT^CGAA AAGC^TT	5' - GC
Bpu95I	FnuDII	CGCG	CG^CG GC^GC	Blunt
Bpu1102I	EspI	GCTNAGC	GC^TNAGC CGANT^CG	5' - ANT
BpuAI	BbvII	GAAGAC	GAAGACNN^NNNN CTTCTGNNNNNN^	5' - NNNN
BpuAmI	SacI	GAGCTC	GAG^CTC CTC^GAG	Blunt
BpuB5I	SplI	CGTACG	C^GTACG GCATG^C	5' - CATG
BpuDI	Bpu10I	CCTNAGC	CC^TNAGC GGANT^CG	5' - ANT
BpuEI	Bce83I	CTTGAG	CTTGAGNNNNNNNNNNNNNNNN^ GAACTCNNNNNNNNNNNNNN^NN	NN - 3'
BpuSI	FinI	GGGAC	GGGACNNNNNNNNNN^NNNN CCCTGNNNNNNNNNNNNNN^	5' - NNNN
BsaI	Eco31I	GGTCTC	GGTCTCN^NNNN CCAGAGNNNNN^	5' - NNNN
Bsa29I	ClaI	ATCGAT	AT^CGAT TAGC^TA	5' - GC
BsaAI		YACGTR	YAC^GTR YTG^CAR	Blunt
BsaBI		GATNNNNATC	GATNN^NNATC CTANN^NNTAG	Blunt
BsaHI	AcyI	GRCGYC	GR^CGYC CRGC^YG	5' - GC
BsaJI	SecI	CCNNGG	C^CNNGG GGNNC^C	5' - GNNC
BsaMI	BsmI	GAATGC	GAATGCN^ CTTAC^GN	CN - 3'
BsaOI	McrI	CGRYCG	CGRY^CG GC^RYGC	RY - 3'
BsaWI	BetI	WCCGGW	W^CCGGW WGGCC^W	5' - GGCC
BsaXI		ACNNNNNCTCC	ACNNNNNCTCCNNNNNNNNNN^ TGNNNNNGAGGNNNNNNN^NNN	NNN - 3'
BscI	ClaI	ATCGAT	AT^CGAT TAGC^TA	5' - GC
Bsc4I	BsiYI	CCNNNNNNNGG	CCNNNNN^NNGG GGNN^NNNNNCC	NNN - 3'
Bsc91I	BbvII	GAAGAC	GAAGACNN^NNNN CTTCTGNNNNNN^	5' - NNNN
Bsc107I	BsiYI	CCNNNNNNNGG	CCNNNNN^NNGG GGNN^NNNNNCC	NNN - 3'
BscAI	SfaNI	GCATC	GCATCNNNN^NN CGTAGNNNNNN^	5' - NN
BscBI	NlaIV	GGNNCC	GGN^NCC CCN^NGG	Blunt
BscCI	BsmI	GAATGC	GAATGCN^ CTTAC^GN	CN - 3'
BscFI	MboI	GATC	^GATC CTAG^	5' - CTAG
Bse1I	BsrI	ACTGG	ACTGGN^ TGAC^CN	GN - 3'
Bse8I	BsaBI	GATNNNNATC	GATNN^NNATC CTANN^NNTAG	Blunt
Bse15I	AvaI	CYCGRG	C^YCGRG GYGCR^C	5' - YGCR
Bse16I	EcoRII	CCWGG	CC^WGG GGW^CC	5' - W
Bse17I	EcoRII	CCWGG	CC^WGG GGW^CC	5' - W
Bse21I	SauI	CCTNAGG	CC^TNAGG GGANT^CC	5' - ANT
Bse24I	EcoRII	CCWGG	CC^WGG GGW^CC	5' - W
Bse64I	BstEII	GGTNACC	G^GTNACC CCANTG^G	5' - CANTG
Bse118I	Cfr10I	RCCGGY	R^CCGGY RGGCC^Y	5' - GGCC
Bse634I	Cfr10I	RCCGGY	R^CCGGY RGGCC^Y	5' - GGCC
BseAI	BspMII	TCCGGA	T^CCGGA AGGCC^T	5' - GGCC
BseBI	EcoRII	CCWGG	CC^WGG GGW^CC	5' - W
BseCI	ClaI	ATCGAT	AT^CGAT TAGC^TA	5' - GC
BseDI	SecI	CCNNGG	C^CNNGG GGNNC^C	5' - GNNC
Bse3DI	BsrDI	GCAATG	GCAATGNN^ CGTTAC^NN	NN - 3'
BseGI	FokI	GGATG	GGATGNN^ CCTAC^NN	NN - 3'
BseJI	BsaBI	GATNNNNATC	GATNN^NNATC CTANN^NNTAG	Blunt
BseKI	BbvI	GCAGC	GCAGCNNNNNNNN^NNNN CGTCGNNNNNNNNNNNN^	5' - NNNN
BseLI	BsiYI	CCNNNNNNNGG	CCNNNNN^NNGG GGNN^NNNNNCC	NNN - 3'
BseMI	BsrDI	GCAATG	GCAATGNN^ CGTTAC^NN	NN - 3'
BseMII		CTCAG	CTCAGNNNNNNNNNN^ GAGTCNNNNNNNN^NN	NN - 3'
BseNI	BsrI	ACTGG	ACTGGN^ TGAC^CN	GN - 3'
BsePI		GCGCGC	G^CGCGC CGCGC^G	5' - GCGC
BseQI	HaeIII	GGCC	GG^CC CC^GG	Blunt
BseRI		GAGGAG	GAGGAGNNNNNNNNNN^ CTCCTCNNNNNNNN^NN	NN - 3'
BseSI		GKGCMC	GKGCM^C C^KCGMG	KGCM - 3'
BseT9I	BstEII	GGTNACC	G^GTNACC CCANTG^G	5' - CANTG
BseT10I	BstEII	GGTNACC	G^GTNACC CCANTG^G	5' - CANTG
BseXI	BbvI	GCAGC	GCAGCNNNNNNNN^NNNN CGTCGNNNNNNNNNNNN^	5' - NNNN
BseX3I	XmaIII	CGGCCG	C^GGCCG GCCGG^C	5' - CCGG
BseYI		CCCAGC	C^CCAGC GGGTC^G	5' - GGTC
BseZI	Ksp632I	CTCTTC	CTCTTCN^NNN GAGAAGNNNN^	5' - NNN
BsgI		GTGCAG	GTGCAGNNNNNNNNNNNNNNNN^ CACGTCNNNNNNNNNNNNNN^NN	NN - 3'
BshI	HaeIII	GGCC	GG^CC CC^GG	Blunt
Bsh45I	HgiAI	GWGCWC	GWGCW^C C^WCGWG	WGCW - 3'
Bsh1236I	FnuDII	CGCG	CG^CG GC^GC	Blunt
Bsh1285I	McrI	CGRYCG	CGRY^CG GC^RYGC	RY - 3'
Bsh1365I	BsaBI	GATNNNNATC	GATNN^NNATC CTANN^NNTAG	Blunt
BshFI	HaeIII	GGCC	GG^CC CC^GG	Blunt
BshGI	EcoRII	CCWGG	CC^WGG GGW^CC	5' - W
BshKI	AsuI	GGNCC	G^GNCC CCNG^G	5' - CNG
BshNI	HgiCI	GGYRCC	G^GYRCC CCYRG^G	5' - CYRG
BshTI	AgeI	ACCGGT	A^CCGGT TGGCC^A	5' - GGCC
BsiI		CACGAG	C^ACGAG GTGCT^C	5' - TGCT
BsiBI	BsaBI	GATNNNNATC	GATNN^NNATC CTANN^NNTAG	Blunt
BsiCI	AsuII	TTCGAA	TT^CGAA AAGC^TT	5' - GC
BsiEI	McrI	CGRYCG	CGRY^CG GC^RYGC	RY - 3'
BsiHKAI	HgiAI	GWGCWC	GWGCW^C C^WCGWG	WGCW - 3'
BsiHKCI	AvaI	CYCGRG	C^YCGRG GYGCR^C	5' - YGCR
BsiKI	BstEII	GGTNACC	G^GTNACC CCANTG^G	5' - CANTG
BsiLI	EcoRII	CCWGG	CC^WGG GGW^CC	5' - W
BsiMI	BspMII	TCCGGA	T^CCGGA AGGCC^T	5' - GGCC
BsiQI	BclI	TGATCA	T^GATCA ACTAG^T	5' - CTAG
BsiSI	HpaII	CCGG	C^CGG GGC^C	5' - GC
BsiWI	SplI	CGTACG	C^GTACG GCATG^C	5' - CATG
BsiXI	ClaI	ATCGAT	AT^CGAT TAGC^TA	5' - GC
BsiYI		CCNNNNNNNGG	CCNNNNN^NNGG GGNN^NNNNNCC	NNN - 3'
BsiZI	AsuI	GGNCC	G^GNCC CCNG^G	5' - CNG
BslI	BsiYI	CCNNNNNNNGG	CCNNNNN^NNGG GGNN^NNNNNCC	NNN - 3'
BslFI	FinI	GGGAC	GGGACNNNNNNNNNN^NNNN CCCTGNNNNNNNNNNNNNN^	5' - NNNN
BsmI		GAATGC	GAATGCN^ CTTAC^GN	CN - 3'
BsmAI		GTCTC	GTCTCN^NNNN CAGAGNNNNN^	5' - NNNN
BsmBI	Esp3I	CGTCTC	CGTCTCN^NNNN GCAGAGNNNNN^	5' - NNNN
BsmFI	FinI	GGGAC	GGGACNNNNNNNNNN^NNNN CCCTGNNNNNNNNNNNNNN^	5' - NNNN
BsmSI	StyI	CCWWGG	C^CWWGG GGWWC^C	5' - GWWC
Bso31I	Eco31I	GGTCTC	GGTCTCN^NNNN CCAGAGNNNNN^	5' - NNNN
BsoBI	AvaI	CYCGRG	C^YCGRG GYGCR^C	5' - YGCR
BsoCI	SduI	GDGCHC	GDGCH^C C^DCGHG	DGCH - 3'
BsoFI	Fnu4HI	GCNGC	GC^NGC CGN^CG	5' - N
BsoMAI	BsmAI	GTCTC	GTCTCN^NNNN CAGAGNNNNN^	5' - NNNN
Bsp6I	Fnu4HI	GCNGC	GC^NGC CGN^CG	5' - N
Bsp13I	BspMII	TCCGGA	T^CCGGA AGGCC^T	5' - GGCC
Bsp19I	NcoI	CCATGG	C^CATGG GGTAC^C	5' - GTAC
Bsp24I		GACNNNNNNTGG	GACNNNNNNTGGNNNNNNNNNNNN^ CTGNNNNNNACCNNNNNNN^NNNNN	NNNNN - 3'
Bsp50I	FnuDII	CGCG	CG^CG GC^GC	Blunt
Bsp63I	PstI	CTGCAG	CTGCA^G G^ACGTC	TGCA - 3'
Bsp67I	MboI	GATC	^GATC CTAG^	5' - CTAG
Bsp68I	NruI	TCGCGA	TCG^CGA AGC^GCT	Blunt
Bsp98I	BamHI	GGATCC	G^GATCC CCTAG^G	5' - CTAG
Bsp105I	MboI	GATC	^GATC CTAG^	5' - CTAG
Bsp106I	ClaI	ATCGAT	AT^CGAT TAGC^TA	5' - GC
Bsp119I	AsuII	TTCGAA	TT^CGAA AAGC^TT	5' - GC
Bsp120I	ApaI	GGGCCC	G^GGCCC CCCGG^G	5' - CCGG
Bsp123I	FnuDII	CGCG	CG^CG GC^GC	Blunt
Bsp143I	MboI	GATC	^GATC CTAG^	5' - CTAG
Bsp143II	HaeII	RGCGCY	RGCGC^Y R^CGCGY	GCGC - 3'
Bsp211I	HaeIII	GGCC	GG^CC CC^GG	Blunt
Bsp423I	BbvI	GCAGC	GCAGCNNNNNNNN^NNNN CGTCGNNNNNNNNNNNN^	5' - NNNN
Bsp519I	HgiJII	GRGCYC	GRGCY^C C^RCGYG	RGCY - 3'
Bsp1286I	SduI	GDGCHC	GDGCH^C C^DCGHG	DGCH - 3'
Bsp1407I		TGTACA	T^GTACA ACATG^T	5' - CATG
Bsp1720I	EspI	GCTNAGC	GC^TNAGC CGANT^CG	5' - ANT
Bsp1894I	AsuI	GGNCC	G^GNCC CCNG^G	5' - CNG
Bsp2095I	MboI	GATC	^GATC CTAG^	5' - CTAG
Bsp4009I	BamHI	GGATCC	G^GATCC CCTAG^G	5' - CTAG
BspAI	MboI	GATC	^GATC CTAG^	5' - CTAG
BspA2I	AvrII	CCTAGG	C^CTAGG GGATC^C	5' - GATC
Bsp153AI	PvuII	CAGCTG	CAG^CTG GTC^GAC	Blunt
BspAAI	XhoI	CTCGAG	C^TCGAG GAGCT^C	5' - AGCT
BspAAII	XbaI	TCTAGA	T^CTAGA AGATC^T	5' - GATC
BspAAIII	BamHI	GGATCC	G^GATCC CCTAG^G	5' - CTAG
BspANI	HaeIII	GGCC	GG^CC CC^GG	Blunt
BspBI	PstI	CTGCAG	CTGCA^G G^ACGTC	TGCA - 3'
BspBII	AsuI	GGNCC	G^GNCC CCNG^G	5' - CNG
BspBRI	HaeIII	GGCC	GG^CC CC^GG	Blunt
BspBS31I	BbvII	GAAGAC	GAAGACNN^NNNN CTTCTGNNNNNN^	5' - NNNN
BspCI	PvuI	CGATCG	CGAT^CG GC^TAGC	AT - 3'
BspCNI	BseMII	CTCAG	CTCAGNNNNNNNNN^ GAGTCNNNNNNN^NN	NN - 3'
BspDI	ClaI	ATCGAT	AT^CGAT TAGC^TA	5' - GC
BspEI	BspMII	TCCGGA	T^CCGGA AGGCC^T	5' - GGCC
BspFI	MboI	GATC	^GATC CTAG^	5' - CTAG
BspF4I	AsuI	GGNCC	G^GNCC CCNG^G	5' - CNG
BspHI		TCATGA	T^CATGA AGTAC^T	5' - GTAC
BspIS4I	BbvII	GAAGAC	GAAGACNN^NNNN CTTCTGNNNNNN^	5' - NNNN
BspJI	MboI	GATC	^GATC CTAG^	5' - CTAG
BspJII	ClaI	ATCGAT	AT^CGAT TAGC^TA	5' - GC
BspKI	HaeIII	GGCC	GG^CC CC^GG	Blunt
BspKT5I	Eco57I	CTGAAG	CTGAAGNNNNNNNNNNNNNNNN^ GACTTCNNNNNNNNNNNNNN^NN	NN - 3'
BspKT6I	MboI	GATC	GAT^C C^TAG	AT - 3'
BspKT8I	HindIII	AAGCTT	A^AGCTT TTCGA^A	5' - TCGA
BspLI	NlaIV	GGNNCC	GGN^NCC CCN^NGG	Blunt
BspLAI	HhaI	GCGC	GCG^C C^GCG	CG - 3'
BspLAII	AsuII	TTCGAA	TT^CGAA AAGC^TT	5' - GC
BspLAIII	HindIII	AAGCTT	A^AGCTT TTCGA^A	5' - TCGA
BspLS2I	SduI	GDGCHC	GDGCH^C C^DCGHG	DGCH - 3'
BspLU4I	AvaI	CYCGRG	C^YCGRG GYGCR^C	5' - YGCR
BspLU11I		ACATGT	A^CATGT TGTAC^A	5' - GTAC
BspLU11III	FinI	GGGAC	GGGACNNNNNNNNNN^NNNN CCCTGNNNNNNNNNNNNNN^	5' - NNNN
BspMI		ACCTGC	ACCTGCNNNN^NNNN TGGACGNNNNNNNN^	5' - NNNN
BspMII		TCCGGA	T^CCGGA AGGCC^T	5' - GGCC
BspM39I	PvuII	CAGCTG	CAG^CTG GTC^GAC	Blunt
BspM90I	SnaI	GTATAC	GTA^TAC CAT^ATG	Blunt
BspMAI	PstI	CTGCAG	CTGCA^G G^ACGTC	TGCA - 3'
BspMKI	SalI	GTCGAC	G^TCGAC CAGCT^G	5' - AGCT
BspNI	EcoRII	CCWGG	CC^WGG GGW^CC	5' - W
BspO4I	PvuII	CAGCTG	CAG^CTG GTC^GAC	Blunt
BspOVI	Eam1105I	GACNNNNNGTC	GACNNN^NNGTC CTGNN^NNNCAG	N - 3'
BspOVII	ClaI	ATCGAT	AT^CGAT TAGC^TA	5' - GC
BspPI	BinI	GGATC	GGATCNNNN^N CCTAGNNNNN^	5' - N
BspRI	HaeIII	GGCC	GG^CC CC^GG	Blunt
BspR7I	SauI	CCTNAGG	CC^TNAGG GGANT^CC	5' - ANT
BspST5I	SfaNI	GCATC	GCATCNNNNN^NNNN CGTAGNNNNNNNNN^	5' - NNNN
BspTI	AflII	CTTAAG	C^TTAAG GAATT^C	5' - AATT
BspT104I	AsuII	TTCGAA	TT^CGAA AAGC^TT	5' - GC
BspT107I	HgiCI	GGYRCC	G^GYRCC CCYRG^G	5' - CYRG
BspTNI	Eco31I	GGTCTC	GGTCTCN^NNNN CCAGAGNNNNN^	5' - NNNN
BspTS514I	BbvII	GAAGAC	GAAGACNN^NNNN CTTCTGNNNNNN^	5' - NNNN
BspWI	MwoI	GCNNNNNNNGC	GCNNNNN^NNGC CGNN^NNNNNCG	NNN - 3'
BspXI	ClaI	ATCGAT	AT^CGAT TAGC^TA	5' - GC
BspXII	BclI	TGATCA	T^GATCA ACTAG^T	5' - CTAG
BspZEI	ClaI	ATCGAT	AT^CGAT TAGC^TA	5' - GC
BsrI		ACTGG	ACTGGN^ TGAC^CN	GN - 3'
BsrAI	AvaII	GGWCC	G^GWCC CCWG^G	5' - CWG
BsrBI		CCGCTC	CCG^CTC GGC^GAG	Blunt
BsrBRI	BsaBI	GATNNNNATC	GATNN^NNATC CTANN^NNTAG	Blunt
BsrDI		GCAATG	GCAATGNN^ CGTTAC^NN	NN - 3'
BsrFI	Cfr10I	RCCGGY	R^CCGGY RGGCC^Y	5' - GGCC
BsrGI	Bsp1407I	TGTACA	T^GTACA ACATG^T	5' - CATG
BsrSI	BsrI	ACTGG	ACTGGN^ TGAC^CN	GN - 3'
BssAI	Cfr10I	RCCGGY	R^CCGGY RGGCC^Y	5' - GGCC
BssECI	SecI	CCNNGG	C^CNNGG GGNNC^C	5' - GNNC
BssHI	XhoI	CTCGAG	C^TCGAG GAGCT^C	5' - AGCT
BssHII	BsePI	GCGCGC	G^CGCGC CGCGC^G	5' - GCGC
BssIMI	SimI	GGGTC	GG^GTC CCCAG^	5' - CAG
BssKI	ScrFI	CCNGG	^CCNGG GGNCC^	5' - GGNCC
BssNAI	SnaI	GTATAC	GTA^TAC CAT^ATG	Blunt
BssSI	BsiI	CACGAG	C^ACGAG GTGCT^C	5' - TGCT
BssT1I	StyI	CCWWGG	C^CWWGG GGWWC^C	5' - GWWC
BstI	BamHI	GGATCC	G^GATCC CCTAG^G	5' - CTAG
Bst1I	EcoRII	CCWGG	CC^WGG GGW^CC	5' - W
Bst2I	EcoRII	CCWGG	CC^WGG GGW^CC	5' - W
Bst6I	Ksp632I	CTCTTC	CTCTTCN^NNN GAGAAGNNNN^	5' - NNN
Bst11I	BsrI	ACTGG	ACTGGN^ TGAC^CN	GN - 3'
Bst12I	BbvI	GCAGC	GCAGCNNNNNNNN^NNNN CGTCGNNNNNNNNNNNN^	5' - NNNN
Bst19I	SfaNI	GCATC	GCATCNNNN^NN CGTAGNNNNNN^	5' - NN
Bst19II	MboI	GATC	^GATC CTAG^	5' - CTAG
Bst28I	ClaI	ATCGAT	AT^CGAT TAGC^TA	5' - GC
Bst38I	EcoRII	CCWGG	CC^WGG GGW^CC	5' - W
Bst40I	HpaII	CCGG	C^CGG GGC^C	5' - GC
Bst71I	BbvI	GCAGC	GCAGCNNNNNNNN^NNNN CGTCGNNNNNNNNNNNN^	5' - NNNN
Bst98I	AflII	CTTAAG	C^TTAAG GAATT^C	5' - AATT
Bst100I	EcoRII	CCWGG	CC^WGG GGW^CC	5' - W
Bst1107I	SnaI	GTATAC	GTA^TAC CAT^ATG	Blunt
BstACI	AcyI	GRCGYC	GR^CGYC CRGC^YG	5' - GC
BstAPI	ApaBI	GCANNNNNTGC	GCANNNN^NTGC CGTN^NNNNACG	NNN - 3'
BstAUI	Bsp1407I	TGTACA	T^GTACA ACATG^T	5' - CATG
BstBI	AsuII	TTCGAA	TT^CGAA AAGC^TT	5' - GC
Bst2BI	BsiI	CACGAG	C^ACGAG GTGCT^C	5' - TGCT
BstBAI	BsaAI	YACGTR	YAC^GTR YTG^CAR	Blunt
BstBSI	SnaI	GTATAC	GTA^TAC CAT^ATG	Blunt
BstB7SI	Cfr10I	RCCGGY	R^CCGGY RGGCC^Y	5' - GGCC
BstBS32I	BbvII	GAAGAC	GAAGACNN^NNNN CTTCTGNNNNNN^	5' - NNNN
BstBZ153I	BsePI	GCGCGC	G^CGCGC CGCGC^G	5' - GCGC
Bst4CI	Tsp4CI	ACNGT	ACN^GT TG^NCA	N - 3'
BstC8I	Cac8I	GCNNGC	GCN^NGC CGN^NCG	Blunt
BstD102I	BsrBI	CCGCTC	CCG^CTC GGC^GAG	Blunt
BstDEI	DdeI	CTNAG	C^TNAG GANT^C	5' - ANT
BstDSI	DsaI	CCRYGG	C^CRYGG GGRYC^C	5' - GRYC
BstEII		GGTNACC	G^GTNACC CCANTG^G	5' - CANTG
BstENI	EcoNI	CCTNNNNNAGG	CCTNN^NNNAGG GGANNN^NNTCC	5' - N
BstENII	MboI	GATC	^GATC CTAG^	5' - CTAG
BstEZ359I	HpaI	GTTAAC	GTT^AAC CAA^TTG	Blunt
BstFI	HindIII	AAGCTT	A^AGCTT TTCGA^A	5' - TCGA
BstF5I	FokI	GGATG	GGATGNN^ CCTAC^NN	NN - 3'
BstFNI	FnuDII	CGCG	CG^CG GC^GC	Blunt
BstFZ438I	FauI	CCCGC	CCCGCNNNN^NN GGGCGNNNNNN^	5' - NN
BstGZ53I	Esp3I	CGTCTC	CGTCTCN^NNNN GCAGAGNNNNN^	5' - NNNN
BstH2I	HaeII	RGCGCY	RGCGC^Y R^CGCGY	GCGC - 3'
BstH9I	BinI	GGATC	GGATCNNNN^N CCTAGNNNNN^	5' - N
BstHHI	HhaI	GCGC	GCG^C C^GCG	CG - 3'
BstHPI	HpaI	GTTAAC	GTT^AAC CAA^TTG	Blunt
BstHZ55I	BstXI	CCANNNNNNTGG	CCANNNNN^NTGG GGTN^NNNNNACC	NNNN - 3'
BstIZ316I	DraIII	CACNNNGTG	CACNNN^GTG GTG^NNNCAC	NNN - 3'
BstJZ301I	DdeI	CTNAG	C^TNAG GANT^C	5' - ANT
BstKTI	MboI	GATC	GAT^C C^TAG	AT - 3'
BstM6I	EcoRII	CCWGG	CC^WGG GGW^CC	5' - W
BstMBI	MboI	GATC	^GATC CTAG^	5' - CTAG
BstMCI	McrI	CGRYCG	CGRY^CG GC^RYGC	RY - 3'
BstMWI	MwoI	GCNNNNNNNGC	GCNNNNN^NNGC CGNN^NNNNNCG	NNN - 3'
BstMZ611I	ScrFI	CCNGG	^CCNGG GGNCC^	5' - GGNCC
BstNI	EcoRII	CCWGG	CC^WGG GGW^CC	5' - W
Bst31NI	BsrBI	CCGCTC	CCG^CTC GGC^GAG	Blunt
BstNSI	NspI	RCATGY	RCATG^Y R^GTACY	CATG - 3'
BstNZ169I	ClaI	ATCGAT	AT^CGAT TAGC^TA	5' - GC
BstOI	EcoRII	CCWGG	CC^WGG GGW^CC	5' - W
BstOZ616I	FinI	GGGAC	GGGACNNNNNNNNNN^NNNN CCCTGNNNNNNNNNNNNNN^	5' - NNNN
BstPI	BstEII	GGTNACC	G^GTNACC CCANTG^G	5' - CANTG
BstPAI	PshAI	GACNNNNGTC	GACNN^NNGTC CTGNN^NNCAG	Blunt
BstPZ418I	FokI	GGATG	GGATGNNNNNNNNN^NNNN CCTACNNNNNNNNNNNNN^	5' - NNNN
BstPZ740I	AflII	CTTAAG	C^TTAAG GAATT^C	5' - AATT
BstRZ246I	SwaI	ATTTAAAT	ATTT^AAAT TAAA^TTTA	Blunt
BstSI	AvaI	CYCGRG	C^YCGRG GYGCR^C	5' - YGCR
BstSCI	ScrFI	CCNGG	^CCNGG GGNCC^	5' - GGNCC
BstSFI	SfeI	CTRYAG	C^TRYAG GARYT^C	5' - ARYT
BstSNI	SnaBI	TACGTA	TAC^GTA ATG^CAT	Blunt
BstSWI	SwaI	ATTTAAAT	ATTT^AAAT TAAA^TTTA	Blunt
BstT7I	BclI	TGATCA	T^GATCA ACTAG^T	5' - CTAG
BstT9I	BstEII	GGTNACC	G^GTNACC CCANTG^G	5' - CANTG
BstT10I	BstEII	GGTNACC	G^GTNACC CCANTG^G	5' - CANTG
Bst31TI	BinI	GGATC	GGATCNNNN^N CCTAGNNNNN^	5' - N
BstTS5I	BbvII	GAAGAC	GAAGACNN^NNNN CTTCTGNNNNNN^	5' - NNNN
BstUI	FnuDII	CGCG	CG^CG GC^GC	Blunt
Bst2UI	EcoRII	CCWGG	CC^WGG GGW^CC	5' - W
BstVI	XhoI	CTCGAG	C^TCGAG GAGCT^C	5' - AGCT
BstV1I	BbvI	GCAGC	GCAGCNNNNNNNN^NNNN CGTCGNNNNNNNNNNNN^	5' - NNNN
BstV2I	BbvII	GAAGAC	GAAGACNN^NNNN CTTCTGNNNNNN^	5' - NNNN
BstXI		CCANNNNNNTGG	CCANNNNN^NTGG GGTN^NNNNNACC	NNNN - 3'
BstX2I	XhoII	RGATCY	R^GATCY RCTAG^Y	5' - CTAG
BstYI	XhoII	RGATCY	R^GATCY RCTAG^Y	5' - CTAG
BstZI	XmaIII	CGGCCG	C^GGCCG GCCGG^C	5' - CCGG
BstZ17I	SnaI	GTATAC	GTA^TAC CAT^ATG	Blunt
Bsu6I	Ksp632I	CTCTTC	CTCTTCN^NNN GAGAAGNNNN^	5' - NNN
Bsu15I	ClaI	ATCGAT	AT^CGAT TAGC^TA	5' - GC
Bsu23I	BspMII	TCCGGA	T^CCGGA AGGCC^T	5' - GGCC
Bsu36I	SauI	CCTNAGG	CC^TNAGG GGANT^CC	5' - ANT
Bsu54I	AsuI	GGNCC	G^GNCC CCNG^G	5' - CNG
Bsu1532I	FnuDII	CGCG	CG^CG GC^GC	Blunt
Bsu1854I	HgiJII	GRGCYC	GRGCY^C C^RCGYG	RGCY - 3'
BsuBI	PstI	CTGCAG	CTGCA^G G^ACGTC	TGCA - 3'
BsuFI	HpaII	CCGG	C^CGG GGC^C	5' - GC
BsuRI	HaeIII	GGCC	GG^CC CC^GG	Blunt
BsuTUI	ClaI	ATCGAT	AT^CGAT TAGC^TA	5' - GC
BteI	HaeIII	GGCC	GG^CC CC^GG	Blunt
BtgI	DsaI	CCRYGG	C^CRYGG GGRYC^C	5' - GRYC
BtgZI		GCGATG	GCGATGNNNNNNNNNN^NNNN CGCTACNNNNNNNNNNNNNN^	5' - NNNN
BthAI	AvaII	GGWCC	G^GWCC CCWG^G	5' - CWG
BthCI	Fnu4HI	GCNGC	GCNG^C C^GNCG	CNG - 3'
BthDI	EcoRII	CCWGG	CC^WGG GGW^CC	5' - W
BthEI	EcoRII	CCWGG	CC^WGG GGW^CC	5' - W
BtkI	FnuDII	CGCG	CG^CG GC^GC	Blunt
BtkII	MboI	GATC	^GATC CTAG^	5' - CTAG
BtrI		CACGTC	CAC^GTC GTG^CAG	Blunt
BtsI		GCAGTG	GCAGTGNN^ CGTCAC^NN	NN - 3'
BveI	BspMI	ACCTGC	ACCTGCNNNN^NNNN TGGACGNNNNNNNN^	5' - NNNN
BvuI	HgiJII	GRGCYC	GRGCY^C C^RCGYG	RGCY - 3'
BvuBI	SplI	CGTACG	C^GTACG GCATG^C	5' - CATG
BaeGI	BseSI	GKGCMC	GKGCM^C C^KCGMG	KGCM - 3'
BciT130I	EcoRII	CCWGG	CC^WGG GGW^CC	5' - W
BcoDI	BsmAI	GTCTC	GTCTCN^NNNN CAGAGNNNNN^	5' - NNNN
BisI	Fnu4HI	GCNGC	GC^NGC CGN^CG	5' - N
BlsI		GCNGC	GCN^GC CG^NCG	N - 3'
BmcAI	ScaI	AGTACT	AGT^ACT TCA^TGA	Blunt
BmeRI	AhdI	GACNNNNNGTC	GACNNN^NNGTC CTGNN^NNNCAG	N - 3'
BmeT110I		CYCGRG	CY^CGRG GYGC^RC	5' - GC
BmgT120I		GGNCC	GG^NCC CCN^GG	5' - N
BmiI		GGNNCC	GGN^NCC CCN^NGG	Blunt
BmrFI		CCNGG	CC^NGG GGN^CC	5' - N
BmuI		ACTGGG（NNNN）N	ACTGGG（NN^NN）N TGACCC（N^NNN）N	N - 3'
BpuMI	CauII	CCSGG	CC^SGG GGS^CC	5' - S
BpvUI	PvuI	CGATCG	CGAT^CG GC^TAGC	AT - 3'
BshVI	ClaI	ATCGAT	AT^CGAT TAGC^TA	5' - GC
BsnI	HaeIII	GGCC	GG^CC CC^GG	Blunt
BspACI	AciI	CCGC	C^CGC GGC^G	5' - GC
BspFNI	FnuDII	CGCG	CG^CG GC^GC	Blunt
BspOI		GCTAGC	GCTAG^C C^GATCG	CTAG - 3'
BspQI		GCTCTTCNNNN	GCTCTTCN^NNN CGAGAAGNNNN^	5' - NNN
BssMI	MboI	GATC	^GATC CTAG^	5' - CTAG
BssNI		GRCGYC	GR^CGYC CRGC^YG	5' - GC
BstAFI		CTTAAG	C^TTAAG GAATT^C	5' - AATT
BstMAI		GTCTCNNNNN	GTCTCN^NNNN CAGAGNNNNN^	5' - NNNN
BstSLI		GKGCMC	GKGCM^C C^KCGMG	KGCM - 3'
BtsCI		GGATGNN	GGATGNN^ CCTAC^NN	NN - 3'
BtsIMutI		CAGTG	CAGTGNN^ GTCAC^NN	NN - 3'
BtuMI		TCGCGA	TCG^CGA TCG^CGA	Blunt
CacI	MboI	GATC	^GATC CTAG^	5' - CTAG
Cac8I		GCNNGC	GCN^NGC CGN^NCG	Blunt
CaiI	AlwNI	CAGNNNCTG	CAGNNN^CTG GTC^NNNGAC	NNN - 3'
CauI	AvaII	GGWCC	G^GWCC CCWG^G	5' - CWG
CauII		CCSGG	CC^SGG GGS^CC	5' - S
CauB3I	BspMII	TCCGGA	T^CCGGA AGGCC^T	5' - GGCC
CbiI	AsuII	TTCGAA	TT^CGAA AAGC^TT	5' - GC
CboI	HpaII	CCGG	C^CGG GGC^C	5' - GC
CbrI	EcoRII	CCWGG	CC^WGG GGW^CC	5' - W
CciNI	NotI	GCGGCCGC	GC^GGCCGC CGCCGG^CG	5' - CCGG
CcoI	NaeI	GCCGGC	GCC^GGC CGG^CCG	Blunt
CcrI	XhoI	CTCGAG	C^TCGAG GAGCT^C	5' - AGCT
CcuI	AsuI	GGNCC	G^GNCC CCNG^G	5' - CNG
CcyI	MboI	GATC	^GATC CTAG^	5' - CTAG
CdiI		CATCG	CATC^G GTAG^C	Blunt
CelI	BamHI	GGATCC	G^GATCC CCTAG^G	5' - CTAG
CelII	EspI	GCTNAGC	GC^TNAGC CGANT^CG	5' - ANT
CeqI	EcoRV	GATATC	GAT^ATC CTA^TAG	Blunt
CflI	PstI	CTGCAG	CTGCA^G G^ACGTC	TGCA - 3'
CfoI	HhaI	GCGC	GCG^C C^GCG	CG - 3'
CfrI		YGGCCR	Y^GGCCR YCCGG^R	5' - CCGG
Cfr6I	PvuII	CAGCTG	CAG^CTG GTC^GAC	Blunt
Cfr9I	SmaI	CCCGGG	C^CCGGG GGGCC^C	5' - GGCC
Cfr10I		RCCGGY	R^CCGGY RGGCC^Y	5' - GGCC
Cfr13I	AsuI	GGNCC	G^GNCC CCNG^G	5' - CNG
Cfr42I	SacII	CCGCGG	CCGC^GG GG^CGCC	GC - 3'
CfrA4I	PstI	CTGCAG	CTGCA^G G^ACGTC	TGCA - 3'
CfrBI	StyI	CCWWGG	C^CWWGG GGWWC^C	5' - GWWC
CfrJ4I	SmaI	CCCGGG	CCC^GGG GGG^CCC	Blunt
CfuI	DpnI	GATC	GA^TC CT^AG	Blunt
CfuII	PstI	CTGCAG	CTGCA^G G^ACGTC	TGCA - 3'
ChaI	MboI	GATC	GATC^ ^CTAG	GATC - 3'
CjeI		CCANNNNNNGT	CCANNNNNNGTNNNNNNNNNNNNNNN^ GGTNNNNNNCANNNNNNNNN^NNNNNN	NNNNNN - 3'
CjePI		CCANNNNNNNTC	CCANNNNNNNTCNNNNNNNNNNNNNN^ GGTNNNNNNNAGNNNNNNNN^NNNNNN	NNNNNN - 3'
ClaI		ATCGAT	AT^CGAT TAGC^TA	5' - GC
CltI	HaeIII	GGCC	GG^CC CC^GG	Blunt
CpfI	MboI	GATC	^GATC CTAG^	5' - CTAG
CpoI	RsrII	CGGWCCG	CG^GWCCG GCCWG^GC	5' - CWG
CscI	SacII	CCGCGG	CCGC^GG GG^CGCC	GC - 3'
CsiAI	AgeI	ACCGGT	A^CCGGT TGGCC^A	5' - GGCC
CsiBI	NotI	GCGGCCGC	GC^GGCCGC CGCCGG^CG	5' - CCGG
CspI	RsrII	CGGWCCG	CG^GWCCG GCCWG^GC	5' - CWG
Csp6I	RsaI	GTAC	G^TAC CAT^G	5' - AT
Csp45I	AsuII	TTCGAA	TT^CGAA AAGC^TT	5' - GC
CspAI	AgeI	ACCGGT	A^CCGGT TGGCC^A	5' - GGCC
CspBI	NotI	GCGGCCGC	GC^GGCCGC CGCCGG^CG	5' - CCGG
CspCI		CAANNNNNGTGG	CAANNNNNGTGGNNNNNNNNNNNN^ GTTNNNNNCACCNNNNNNNNNN^NN	NN - 3'
Csp68KI	AvaII	GGWCC	G^GWCC CCWG^G	5' - CWG
Csp68KII	AsuII	TTCGAA	TT^CGAA AAGC^TT	5' - GC
Csp68KIII	AvaIII	ATGCAT	ATGCA^T T^ACGTA	TGCA - 3'
Csp68KVI	FnuDII	CGCG	CG^CG GC^GC	Blunt
CspKVI	FnuDII	CGCG	CG^CG GC^GC	Blunt
CstI	PstI	CTGCAG	CTGCA^G G^ACGTC	TGCA - 3'
CstMI		AAGGAG	AAGGAGNNNNNNNNNNNNNNNNNNNN^ TTCCTCNNNNNNNNNNNNNNNNNN^NN	NN - 3'
CthII	EcoRII	CCWGG	CC^WGG GGW^CC	5' - W
CviAI	MboI	GATC	^GATC CTAG^	5' - CTAG
CviAII	NlaIII	CATG	C^ATG GTA^C	5' - TA
CviBI	HinfI	GANTC	G^ANTC CTNA^G	5' - TNA
CviJI		RGCY	RG^CY RC^GY	Blunt
CviQI	RsaI	GTAC	G^TAC CAT^G	5' - AT
CviRI		TGCA	TG^CA AC^GT	Blunt
CviRII	RsaI	GTAC	G^TAC CAT^G	5' - AT
CviTI	CviJI	RGCY	RG^CY RC^GY	Blunt
CvnI	SauI	CCTNAGG	CC^TNAGG GGANT^CC	5' - ANT
CciI		TCATGA	T^CATGA TCATG^A	5' - catg
CseI		GACGC	GACGCNNNNNN^NNNNN GACGCNNNNNNNNNNN^	5' - NNNNN
CviKI-1		RGCY	RG^CY RC^GY	Blunt
DdeI		CTNAG	C^TNAG GANT^C	5' - ANT
DmaI	PvuII	CAGCTG	CAG^CTG GTC^GAC	Blunt
DpaI	ScaI	AGTACT	AGT^ACT TCA^TGA	Blunt
DpnI		GATC	GA^TC CT^AG	Blunt
DpnII	MboI	GATC	^GATC CTAG^	5' - CTAG
DraI	AhaIII	TTTAAA	TTT^AAA AAA^TTT	Blunt
DraII		RGGNCCY	RG^GNCCY RCCNG^GY	5' - CNG
DraIII		CACNNNGTG	CACNNN^GTG GTG^NNNCAC	NNN - 3'
DrdI		GACNNNNNNGTC	GACNNNN^NNGTC CTGNN^NNNNCAG	NN - 3'
DriI	Eam1105I	GACNNNNNGTC	GACNNN^NNGTC CTGNN^NNNCAG	N - 3'
DsaI		CCRYGG	C^CRYGG GGRYC^C	5' - GRYC
DsaII	HaeIII	GGCC	GG^CC CC^GG	Blunt
DsaIII	XhoII	RGATCY	R^GATCY RCTAG^Y	5' - CTAG
DsaIV	AvaII	GGWCC	G^GWCC CCWG^G	5' - CWG
DsaV	ScrFI	CCNGG	^CCNGG GGNCC^	5' - GGNCC
DseDI	DrdI	GACNNNNNNGTC	GACNNNN^NNGTC CTGNN^NNNNCAG	NN - 3'
DinI		GGCGCC	GGC^GCC CCG^CGG	Blunt
EacI	BinI	GGATC	GGATCNNNN^N CCTAGNNNNN^	5' - N
EaeI	CfrI	YGGCCR	Y^GGCCR YCCGG^R	5' - CCGG
Eae46I	SacII	CCGCGG	CCGC^GG GG^CGCC	GC - 3'
EaeAI	SmaI	CCCGGG	C^CCGGG GGGCC^C	5' - GGCC
EagI	XmaIII	CGGCCG	C^GGCCG GCCGG^C	5' - CCGG
EagBI	PvuI	CGATCG	CGAT^CG GC^TAGC	AT - 3'
EagMI	AvaII	GGWCC	G^GWCC CCWG^G	5' - CWG
Eam1104I	Ksp632I	CTCTTC	CTCTTCN^NNN GAGAAGNNNN^	5' - NNN
Eam1105I		GACNNNNNGTC	GACNNN^NNGTC CTGNN^NNNCAG	N - 3'
EarI	Ksp632I	CTCTTC	CTCTTCN^NNN GAGAAGNNNN^	5' - NNN
EcaI	BstEII	GGTNACC	G^GTNACC CCANTG^G	5' - CANTG
EciI		GGCGGA	GGCGGANNNNNNNNNNN^ CCGCCTNNNNNNNNN^NN	NN - 3'
Eci125I	BstEII	GGTNACC	G^GTNACC CCANTG^G	5' - CANTG
EclI	PvuII	CAGCTG	CAG^CTG GTC^GAC	Blunt
Ecl136II	SacI	GAGCTC	GAG^CTC CTC^GAG	Blunt
EclHKI	Eam1105I	GACNNNNNGTC	GACNNN^NNGTC CTGNN^NNNCAG	N - 3'
EclRI	SmaI	CCCGGG	C^CCGGG GGGCC^C	5' - GGCC
EclXI	XmaIII	CGGCCG	C^GGCCG GCCGG^C	5' - CCGG
Ecl18kI	ScrFI	CCNGG	^CCNGG GGNCC^	5' - GGNCC
Ecl37kI	PstI	CTGCAG	CTGCA^G G^ACGTC	TGCA - 3'
Ecl2zI	PstI	CTGCAG	CTGCA^G G^ACGTC	TGCA - 3'
Eco24I	HgiJII	GRGCYC	GRGCY^C C^RCGYG	RGCY - 3'
Eco31I		GGTCTC	GGTCTCN^NNNN CCAGAGNNNNN^	5' - NNNN
Eco32I	EcoRV	GATATC	GAT^ATC CTA^TAG	Blunt
Eco47I	AvaII	GGWCC	G^GWCC CCWG^G	5' - CWG
Eco47III		AGCGCT	AGC^GCT TCG^CGA	Blunt
Eco52I	XmaIII	CGGCCG	C^GGCCG GCCGG^C	5' - CCGG
Eco56I	NaeI	GCCGGC	G^CCGGC CGGCC^G	5' - GGCC
Eco57I		CTGAAG	CTGAAGNNNNNNNNNNNNNNNN^ GACTTCNNNNNNNNNNNNNN^NN	NN - 3'
Eco64I	HgiCI	GGYRCC	G^GYRCC CCYRG^G	5' - CYRG
Eco72I	PmaCI	CACGTG	CAC^GTG GTG^CAC	Blunt
Eco78I	NarI	GGCGCC	GGC^GCC CCG^CGG	Blunt
Eco81I	SauI	CCTNAGG	CC^TNAGG GGANT^CC	5' - ANT
Eco88I	AvaI	CYCGRG	C^YCGRG GYGCR^C	5' - YGCR
Eco91I	BstEII	GGTNACC	G^GTNACC CCANTG^G	5' - CANTG
Eco105I	SnaBI	TACGTA	TAC^GTA ATG^CAT	Blunt
Eco130I	StyI	CCWWGG	C^CWWGG GGWWC^C	5' - GWWC
Eco147I	StuI	AGGCCT	AGG^CCT TCC^GGA	Blunt
Eco255I	ScaI	AGTACT	AGT^ACT TCA^TGA	Blunt
Eco1831I	CauII	CCSGG	^CCSGG GGSCC^	5' - GGSCC
EcoA4I	Eco31I	GGTCTC	GGTCTCN^NNNN CCAGAGNNNNN^	5' - NNNN
EcoHI	CauII	CCSGG	^CCSGG GGSCC^	5' - GGSCC
EcoHK31I	CfrI	YGGCCR	Y^GGCCR YCCGG^R	5' - CCGG
EcoICRI	SacI	GAGCTC	GAG^CTC CTC^GAG	Blunt
Eco75KI	HgiJII	GRGCYC	GRGCY^C C^RCGYG	RGCY - 3'
Eco57MI		CTGRAG	CTGRAGNNNNNNNNNNNNNNNN^ GACRTCNNNNNNNNNNNNNN^NN	NN - 3'
EcoNI		CCTNNNNNAGG	CCTNN^NNNAGG GGANNN^NNTCC	5' - N
EcoO44I	Eco31I	GGTCTC	GGTCTCN^NNNN CCAGAGNNNNN^	5' - NNNN
EcoO65I	BstEII	GGTNACC	G^GTNACC CCANTG^G	5' - CANTG
EcoO109I	DraII	RGGNCCY	RG^GNCCY RCCNG^GY	5' - CNG
EcoO128I	BstEII	GGTNACC	G^GTNACC CCANTG^G	5' - CANTG
EcoRI		GAATTC	G^AATTC CTTAA^G	5' - TTAA
EcoRII		CCWGG	^CCWGG GGWCC^	5' - GGWCC
EcoRV		GATATC	GAT^ATC CTA^TAG	Blunt
EcoT14I	StyI	CCWWGG	C^CWWGG GGWWC^C	5' - GWWC
EcoT22I	AvaIII	ATGCAT	ATGCA^T T^ACGTA	TGCA - 3'
EcoT38I	HgiJII	GRGCYC	GRGCY^C C^RCGYG	RGCY - 3'
EcoVIII	HindIII	AAGCTT	A^AGCTT TTCGA^A	5' - TCGA
Eco13kI	ScrFI	CCNGG	^CCNGG GGNCC^	5' - GGNCC
Eco21kI	ScrFI	CCNGG	^CCNGG GGNCC^	5' - GGNCC
Eco27kI	AvaI	CYCGRG	C^YCGRG GYGCR^C	5' - YGCR
Eco29kI	SacII	CCGCGG	CCGC^GG GG^CGCC	GC - 3'
Eco53kI	SacI	GAGCTC	GAG^CTC CTC^GAG	Blunt
Eco137kI	ScrFI	CCNGG	^CCNGG GGNCC^	5' - GGNCC
EgeI	NarI	GGCGCC	GGC^GCC CCG^CGG	Blunt
EheI	NarI	GGCGCC	GGC^GCC CCG^CGG	Blunt
ErhI	StyI	CCWWGG	C^CWWGG GGWWC^C	5' - GWWC
ErhB9I	PvuI	CGATCG	CGAT^CG GC^TAGC	AT - 3'
ErhB9II	StyI	CCWWGG	C^CWWGG GGWWC^C	5' - GWWC
ErpI	AvaII	GGWCC	G^GWCC CCWG^G	5' - CWG
EsaBC3I	TaqI	TCGA	TC^GA AG^CT	Blunt
EsaBC4I	HaeIII	GGCC	GG^CC CC^GG	Blunt
EspI		GCTNAGC	GC^TNAGC CGANT^CG	5' - ANT
Esp3I		CGTCTC	CGTCTCN^NNNN GCAGAGNNNNN^	5' - NNNN
Esp4I	AflII	CTTAAG	C^TTAAG GAATT^C	5' - AATT
Esp1396I	PflMI	CCANNNNNTGG	CCANNNN^NTGG GGTN^NNNNACC	NNN - 3'
EcoP15I		CAGCAG	CAGCAGNNNNNNNNNNNNNNNNNNNNNNNNN^NN GTCGTCNNNNNNNNNNNNNNNNNNNNNNNNNNN^	5' - NN
FalI		AAGNNNNNCTT	AAGNNNNNCTTNNNNNNNNNNNNN^ TTCNNNNNGAANNNNNNNN^NNNNN	NNNNN - 3'
FalII	FnuDII	CGCG	CG^CG GC^GC	Blunt
FaqI	FinI	GGGAC	GGGACNNNNNNNNNN^NNNN CCCTGNNNNNNNNNNNNNN^	5' - NNNN
FatI	NlaIII	CATG	^CATG GTAC^	5' - GTAC
FauI		CCCGC	CCCGCNNNN^NN GGGCGNNNNNN^	5' - NN
FauBII	FnuDII	CGCG	CG^CG GC^GC	Blunt
FauNDI	NdeI	CATATG	CA^TATG GTAT^AC	5' - AT
FbaI	BclI	TGATCA	T^GATCA ACTAG^T	5' - CTAG
FblI	AccI	GTMKAC	GT^MKAC CAMK^TG	5' - MK
FbrI	Fnu4HI	GCNGC	GC^NGC CGN^CG	5' - N
FdiI	AvaII	GGWCC	G^GWCC CCWG^G	5' - CWG
FdiII	MstI	TGCGCA	TGC^GCA ACG^CGT	Blunt
FgoI	MaeI	CTAG	C^TAG GAT^C	5' - AT
FmuI	AsuI	GGNCC	GGNC^C C^CNGG	GNC - 3'
FnuAI	HinfI	GANTC	G^ANTC CTNA^G	5' - TNA
FnuCI	MboI	GATC	^GATC CTAG^	5' - CTAG
FnuDI	HaeIII	GGCC	GG^CC CC^GG	Blunt
FnuDII		CGCG	CG^CG GC^GC	Blunt
FnuDIII	HhaI	GCGC	GCG^C C^GCG	CG - 3'
FnuEI	MboI	GATC	^GATC CTAG^	5' - CTAG
Fnu4HI		GCNGC	GC^NGC CGN^CG	5' - N
FokI		GGATG	GGATGNNNNNNNNN^NNNN CCTACNNNNNNNNNNNNN^	5' - NNNN
FriOI	HgiJII	GRGCYC	GRGCY^C C^RCGYG	RGCY - 3'
FseI		GGCCGGCC	GGCCGG^CC CC^GGCCGG	CCGG - 3'
FsiI	ApoI	RAATTY	R^AATTY RTTAA^Y	5' - TTAA
FspI	MstI	TGCGCA	TGC^GCA ACG^CGT	Blunt
FspII	AsuII	TTCGAA	TT^CGAA AAGC^TT	5' - GC
Fsp1604I	EcoRII	CCWGG	CC^WGG GGW^CC	5' - W
FspAI		RTGCGCAY	RTGC^GCAY RACG^CGTY	Blunt
FspBI	MaeI	CTAG	C^TAG GAT^C	5' - AT
Fsp4HI	Fnu4HI	GCNGC	GC^NGC CGN^CG	5' - N
FspMSI	AvaII	GGWCC	G^GWCC CCWG^G	5' - CWG
FssI	AvaII	GGWCC	G^GWCC CCWG^G	5' - CWG
FunI	Eco47III	AGCGCT	AGC^GCT TCG^CGA	Blunt
FunII	EcoRI	GAATTC	G^AATTC CTTAA^G	5' - TTAA
FaeI		CATG	CATG^ ^GTAC	CATG - 3'
FaiI		YATR	YA^TR YT^AR	Blunt
GalI	SacII	CCGCGG	CCGC^GG GG^CGCC	GC - 3'
GceI	SacII	CCGCGG	CCGC^GG GG^CGCC	GC - 3'
GceGLI	SacII	CCGCGG	CCGC^GG GG^CGCC	GC - 3'
GdiI	StuI	AGGCCT	AGG^CCT TCC^GGA	Blunt
GdiII		CGGCCR	C^GGCCR GCCGG^R	5' - CCGG
GstI	BamHI	GGATCC	G^GATCC CCTAG^G	5' - CTAG
GsuI		CTGGAG	CTGGAGNNNNNNNNNNNNNNNN^ GACCTCNNNNNNNNNNNNNN^NN	NN - 3'
GlaI		GCGC	GC^GC CG^CG	Blunt
GluI	Fnu4HI	GCNGC	GC^NGC CGN^CG	5' - N
GsaI		CCCAGC	CCCAG^C G^GGTCG	CCAG - 3'
HacI	MboI	GATC	^GATC CTAG^	5' - CTAG
HaeI		WGGCCW	WGG^CCW WCC^GGW	Blunt
HaeII		RGCGCY	RGCGC^Y R^CGCGY	GCGC - 3'
HaeIII		GGCC	GG^CC CC^GG	Blunt
HaeIV		GAYNNNNNRTC	GAYNNNNNRTCNNNNNNNNNNNNNN^ CTYNNNNNRAGNNNNNNNNN^NNNNN	NNNNN - 3'
HalI	EcoRI	GAATTC	G^AATTC CTTAA^G	5' - TTAA
HalII	PstI	CTGCAG	CTGCA^G G^ACGTC	TGCA - 3'
HapII	HpaII	CCGG	C^CGG GGC^C	5' - GC
HgaI		GACGC	GACGCNNNNN^NNNNN CTGCGNNNNNNNNNN^	5' - NNNNN
HgiI	AcyI	GRCGYC	GR^CGYC CRGC^YG	5' - GC
HgiAI		GWGCWC	GWGCW^C C^WCGWG	WGCW - 3'
HgiBI	AvaII	GGWCC	G^GWCC CCWG^G	5' - CWG
HgiCI		GGYRCC	G^GYRCC CCYRG^G	5' - CYRG
HgiCII	AvaII	GGWCC	G^GWCC CCWG^G	5' - CWG
HgiCIII	SalI	GTCGAC	G^TCGAC CAGCT^G	5' - AGCT
HgiDI	AcyI	GRCGYC	GR^CGYC CRGC^YG	5' - GC
HgiDII	SalI	GTCGAC	G^TCGAC CAGCT^G	5' - AGCT
HgiEI	AvaII	GGWCC	G^GWCC CCWG^G	5' - CWG
HgiGI	AcyI	GRCGYC	GR^CGYC CRGC^YG	5' - GC
HgiHI	HgiCI	GGYRCC	G^GYRCC CCYRG^G	5' - CYRG
HgiHII	AcyI	GRCGYC	GR^CGYC CRGC^YG	5' - GC
HgiHIII	AvaII	GGWCC	G^GWCC CCWG^G	5' - CWG
HgiJI	AvaII	GGWCC	G^GWCC CCWG^G	5' - CWG
HgiJII		GRGCYC	GRGCY^C C^RCGYG	RGCY - 3'
HgiS22I	CauII	CCSGG	CC^SGG GGS^CC	5' - S
HhaI		GCGC	GCG^C C^GCG	CG - 3'
HhaII	HinfI	GANTC	G^ANTC CTNA^G	5' - TNA
Hin1I	AcyI	GRCGYC	GR^CGYC CRGC^YG	5' - GC
Hin1II	NlaIII	CATG	CATG^ ^GTAC	CATG - 3'
Hin2I	HpaII	CCGG	C^CGG GGC^C	5' - GC
Hin4I		GAYNNNNNVTC	GAYNNNNNVTCNNNNNNNNNNNNN^ CTYNNNNNVAGNNNNNNNN^NNNNN	NNNNN - 3'
Hin6I	HhaI	GCGC	G^CGC CGC^G	5' - GC
HinJCI	HindII	GTYRAC	GTY^RAC CAY^RTG	Blunt
HinP1I	HhaI	GCGC	G^CGC CGC^G	5' - GC
HincII	HindII	GTYRAC	GTY^RAC CAY^RTG	Blunt
HindII		GTYRAC	GTY^RAC CAY^RTG	Blunt
HindIII		AAGCTT	A^AGCTT TTCGA^A	5' - TCGA
HinfI		GANTC	G^ANTC CTNA^G	5' - TNA
HjaI	EcoRV	GATATC	GAT^ATC CTA^TAG	Blunt
HpaI		GTTAAC	GTT^AAC CAA^TTG	Blunt
HpaII		CCGG	C^CGG GGC^C	5' - GC
HphI		GGTGA	GGTGANNNNNNNN^ CCACTNNNNNNN^N	N - 3'
Hpy8I	MjaIV	GTNNAC	GTN^NAC CAN^NTG	Blunt
Hpy51I	Tsp45I	GTSAC	^GTSAC CASTG^	5' - CASTG
Hpy99I		CGWCG	CGWCG^ ^GCWGC	CGWCG - 3'
Hpy178III		TCNNGA	TC^NNGA AGNN^CT	5' - NN
Hpy188I		TCNGA	TCN^GA AG^NCT	N - 3'
Hpy188III	Hpy178III	TCNNGA	TC^NNGA AGNN^CT	5' - NN
HpyAV	Hin4II	CCTTC	CCTTCNNNNNN^ GGAAGNNNNN^N	N - 3'
HpyBI	RsaI	GTAC	GT^AC CA^TG	Blunt
HpyBII	MjaIV	GTNNAC	GTN^NAC CAN^NTG	Blunt
HpyCI	EcoRV	GATATC	GAT^ATC CTA^TAG	Blunt
HpyC1I	BccI	CCATC	CCATCNNNN^N GGTAGNNNNN^	5' - N
HpyCH4I	NlaIII	CATG	CATG^ ^GTAC	CATG - 3'
HpyCH4III	Tsp4CI	ACNGT	ACN^GT TG^NCA	N - 3'
HpyCH4IV	MaeII	ACGT	A^CGT TGC^A	5' - GC
HpyCH4V	CviRI	TGCA	TG^CA AC^GT	Blunt
HpyF10VI	MwoI	GCNNNNNNNGC	GCNNNNN^NNGC CGNN^NNNNNCG	NNN - 3'
HpyF44III	CviRI	TGCA	TG^CA AC^GT	Blunt
HsoI	HhaI	GCGC	G^CGC CGC^G	5' - GC
Hsp92I	AcyI	GRCGYC	GR^CGYC CRGC^YG	5' - GC
Hsp92II	NlaIII	CATG	CATG^ ^GTAC	CATG - 3'
HspAI	HhaI	GCGC	G^CGC CGC^G	5' - GC
HsuI	HindIII	AAGCTT	A^AGCTT TTCGA^A	5' - TCGA
Hpy166II	MjaIV	GTNNAC	GTN^NAC CAN^NTG	Blunt
HpyF3I	DdeI	CTNAG	C^TNAG GANT^C	5' - ANT
ItaI	Fnu4HI	GCNGC	GC^NGC CGN^CG	5' - N
I-CeuI		TAACTATAACGGTCCTAAGGTAGCGA	TAACTATAACGGTCCTAA^GGTAGCGA ATTGATATTGCCAG^GATTCCATCGCT	CTAA - 3'
I-PpoI		TAACTATGACTCTCTTAAGGTAGCCAAAT	TAACTATGACT^CTCTTAAGGTAGCCAAAT ATTGATACTGAGAGAATT^CCATCGGTTTA	5' - GAGAATT
I-SceI		TAGGGATAACAGGGTAAT	TAGGGATAA^CAGGGTAAT ATCCC^TATTGTCCCATTA	ATAA - 3'
KasI	NarI	GGCGCC	G^GCGCC CCGCG^G	5' - CGCG
Kaz48kI	DraII	RGGNCCY	RGGNC^CY RC^CNGGY	GNC - 3'
KoxII	HgiJII	GRGCYC	GRGCY^C C^RCGYG	RGCY - 3'
KpnI		GGTACC	GGTAC^C C^CATGG	GTAC - 3'
Kpn2I	BspMII	TCCGGA	T^CCGGA AGGCC^T	5' - GGCC
Kpn378I	SacII	CCGCGG	CCGC^GG GG^CGCC	GC - 3'
Kpn2kI	ScrFI	CCNGG	^CCNGG GGNCC^	5' - GGNCC
Kpn49kI	EcoRI	GAATTC	G^AATTC CTTAA^G	5' - TTAA
Kpn49kII	CauII	CCSGG	^CCSGG GGSCC^	5' - GGSCC
KspI	SacII	CCGCGG	CCGC^GG GG^CGCC	GC - 3'
Ksp22I	BclI	TGATCA	T^GATCA ACTAG^T	5' - CTAG
Ksp632I		CTCTTC	CTCTTCN^NNN GAGAAGNNNN^	5' - NNN
KspAI	HpaI	GTTAAC	GTT^AAC CAA^TTG	Blunt
Kzo9I	MboI	GATC	^GATC CTAG^	5' - CTAG
Kzo49I	AvaII	GGWCC	G^GWCC CCWG^G	5' - CWG
LcaI	ClaI	ATCGAT	AT^CGAT TAGC^TA	5' - GC
LlaAI	MboI	GATC	^GATC CTAG^	5' - CTAG
LlaBI	SfeI	CTRYAG	C^TRYAG GARYT^C	5' - ARYT
LlaCI	HindIII	AAGCTT	A^AGCTT TTCGA^A	5' - TCGA
LlaG2I	NheI	GCTAGC	G^CTAGC CGATC^G	5' - GATC
Lmu60I	SauI	CCTNAGG	CC^TNAGG GGANT^CC	5' - ANT
LplI	ClaI	ATCGAT	AT^CGAT TAGC^TA	5' - GC
LpnI	HaeII	RGCGCY	RGC^GCY RCG^CGY	Blunt
LspI	AsuII	TTCGAA	TT^CGAA AAGC^TT	5' - GC
LweI	SfaNI	GCATC	GCATCNNNNN^NNNN CGTAGNNNNNNNNN^	5' - NNNN
LguI		GCTCTTCNNNN	GCTCTTCN^NNN CGA^GAAGNNNN	CTTCN - 3'
Lsp1109I		GCAGC(N)8(N)4	GCAGC(N)8(N)^4 CGTC^G(N)8(N)4	C(N)8(N) - 3'
MabI	SexAI	ACCWGGT	A^CCWGGT TGGWCC^A	5' - GGWCC
MaeI		CTAG	C^TAG GAT^C	5' - AT
MaeII		ACGT	A^CGT TGC^A	5' - GC
MaeIII		GTNAC	^GTNAC CANTG^	5' - CANTG
MaeK81I	SplI	CGTACG	C^GTACG GCATG^C	5' - CATG
MaeK81II	AsuI	GGNCC	G^GNCC CCNG^G	5' - CNG
MamI	BsaBI	GATNNNNATC	GATNN^NNATC CTANN^NNTAG	Blunt
MavI	XhoI	CTCGAG	C^TCGAG GAGCT^C	5' - AGCT
MbiI	BsrBI	CCGCTC	CCG^CTC GGC^GAG	Blunt
MboI		GATC	^GATC CTAG^	5' - CTAG
MboII		GAAGA	GAAGANNNNNNNN^ CTTCTNNNNNNN^N	N - 3'
MchI	NarI	GGCGCC	GG^CGCC CCGC^GG	5' - GC
MchAI	NotI	GCGGCCGC	GC^GGCCGC CGCCGG^CG	5' - CCGG
MchAII	HaeIII	GGCC	GG^CC CC^GG	Blunt
McrI		CGRYCG	CGRY^CG GC^RYGC	RY - 3'
MfeI		CAATTG	C^AATTG GTTAA^C	5' - TTAA
MflI	XhoII	RGATCY	R^GATCY RCTAG^Y	5' - CTAG
MfoAI	HaeIII	GGCC	GG^CC CC^GG	Blunt
Mgl14481I	CauII	CCSGG	CC^SGG GGS^CC	5' - S
MgoI	MboI	GATC	^GATC CTAG^	5' - CTAG
MhaAI	PstI	CTGCAG	CTGCA^G G^ACGTC	TGCA - 3'
MhlI	SduI	GDGCHC	GDGCH^C C^DCGHG	DGCH - 3'
MkrAI	MboI	GATC	^GATC CTAG^	5' - CTAG
MlaI	AsuII	TTCGAA	TT^CGAA AAGC^TT	5' - GC
MlaAI	XhoI	CTCGAG	C^TCGAG GAGCT^C	5' - AGCT
MlsI	BalI	TGGCCA	TGG^CCA ACC^GGT	Blunt
MltI	AluI	AGCT	AG^CT TC^GA	Blunt
MluI		ACGCGT	A^CGCGT TGCGC^A	5' - GCGC
Mlu23I	BamHI	GGATCC	G^GATCC CCTAG^G	5' - CTAG
Mlu31I	BalI	TGGCCA	TGG^CCA ACC^GGT	Blunt
MluB2I	NruI	TCGCGA	TCG^CGA AGC^GCT	Blunt
MluNI	BalI	TGGCCA	TGG^CCA ACC^GGT	Blunt
MlyI	PleI	GAGTC	GAGTCNNNNN^ CTCAGNNNNN^	Blunt
Mly113I	NarI	GGCGCC	GG^CGCC CCGC^GG	5' - GC
MmeI		TCCRAC	TCCRACNNNNNNNNNNNNNNNNNNNN^ AGGRTGNNNNNNNNNNNNNNNNNN^NN	NN - 3'
MnlI		CCTC	CCTCNNNNNNN^ GGAGNNNNNN^N	N - 3'
MnoI	HpaII	CCGG	C^CGG GGC^C	5' - GC
Mph1103I	AvaIII	ATGCAT	ATGCA^T T^ACGTA	TGCA - 3'
MroI	BspMII	TCCGGA	T^CCGGA AGGCC^T	5' - GGCC
MroNI	NaeI	GCCGGC	G^CCGGC CGGCC^G	5' - GGCC
MroXI	XmnI	GAANNNNTTC	GAANN^NNTTC CTTNN^NNAAG	Blunt
MscI	BalI	TGGCCA	TGG^CCA ACC^GGT	Blunt
MseI		TTAA	T^TAA AAT^T	5' - AT
MslI		CAYNNNNRTG	CAYNN^NNRTG GTYNN^NNRAC	Blunt
MspI	HpaII	CCGG	C^CGG GGC^C	5' - GC
Msp17I	AcyI	GRCGYC	GR^CGYC CRGC^YG	5' - GC
Msp20I	BalI	TGGCCA	TGG^CCA ACC^GGT	Blunt
Msp67I	ScrFI	CCNGG	CC^NGG GGN^CC	5' - N
MspA1I	NspBII	CMGCKG	CMG^CKG GMC^GKC	Blunt
MspB4I	HgiCI	GGYRCC	G^GYRCC CCYRG^G	5' - CYRG
MspCI	AflII	CTTAAG	C^TTAAG GAATT^C	5' - AATT
MspR9I	ScrFI	CCNGG	CC^NGG GGN^CC	5' - N
MspSWI	SwaI	ATTTAAAT	ATTT^AAAT TAAA^TTTA	Blunt
MspV281I	HgiAI	GWGCWC	GWGCW^C C^WCGWG	WGCW - 3'
MspYI	BsaAI	YACGTR	YAC^GTR YTG^CAR	Blunt
MssI	PmeI	GTTTAAAC	GTTT^AAAC CAAA^TTTG	Blunt
MstI		TGCGCA	TGC^GCA ACG^CGT	Blunt
MstII	SauI	CCTNAGG	CC^TNAGG GGANT^CC	5' - ANT
MthZI	MaeI	CTAG	C^TAG GAT^C	5' - AT
MunI	MfeI	CAATTG	C^AATTG GTTAA^C	5' - TTAA
MvaI	EcoRII	CCWGG	CC^WGG GGW^CC	5' - W
Mva1269I	BsmI	GAATGC	GAATGCN^ CTTAC^GN	CN - 3'
MvnI	FnuDII	CGCG	CG^CG GC^GC	Blunt
MvrI	PvuI	CGATCG	CGAT^CG GC^TAGC	AT - 3'
MwoI		GCNNNNNNNGC	GCNNNNN^NNGC CGNN^NNNNNCG	NNN - 3'
MxaI	SacI	GAGCTC	GAG^CTC CTC^GAG	Blunt
MalI	MboI	GATC	GA^TC CT^AG	Blunt
MauBI		CGCGCGCG	CG^CGCGCG GCGCGC^GC	5' - GCGC
MluCI	TspEI	AATT	^AATT TTAA^	5' - TTAA
MreI	Sse232I	CGCCGGCG	CG^CCGGCG GCGGCC^GC	5' - GGCC
NaeI		GCCGGC	GCC^GGC CGG^CCG	Blunt
NarI		GGCGCC	GG^CGCC CCGC^GG	5' - GC
NblI	PvuI	CGATCG	CGAT^CG GC^TAGC	AT - 3'
NciI	CauII	CCSGG	CC^SGG GGS^CC	5' - S
NcoI		CCATGG	C^CATGG GGTAC^C	5' - GTAC
NcrI	BglII	AGATCT	A^GATCT TCTAG^A	5' - CTAG
NcuI	MboII	GAAGA	GAAGANNNNNNNN^ CTTCTNNNNNNN^N	N - 3'
NdaI	NarI	GGCGCC	GG^CGCC CCGC^GG	5' - GC
NdeI		CATATG	CA^TATG GTAT^AC	5' - AT
NdeII	MboI	GATC	^GATC CTAG^	5' - CTAG
NgoAIII	SacII	CCGCGG	CCGC^GG GG^CGCC	GC - 3'
NgoAIV	NaeI	GCCGGC	G^CCGGC CGGCC^G	5' - GGCC
NgoMIV	NaeI	GCCGGC	G^CCGGC CGGCC^G	5' - GGCC
NgoPII	HaeIII	GGCC	GG^CC CC^GG	Blunt
NgoPIII	SacII	CCGCGG	CCGC^GG GG^CGCC	GC - 3'
NheI		GCTAGC	G^CTAGC CGATC^G	5' - GATC
NlaII	MboI	GATC	^GATC CTAG^	5' - CTAG
NlaIII		CATG	CATG^ ^GTAC	CATG - 3'
NlaIV		GGNNCC	GGN^NCC CCN^NGG	Blunt
Nli3877I	AvaI	CYCGRG	CYCGR^G G^YGCRC	YCGR - 3'
NmeCI	MboI	GATC	^GATC CTAG^	5' - CTAG
NmeRI	PvuII	CAGCTG	CAG^CTG GTC^GAC	Blunt
NmuCI	Tsp45I	GTSAC	^GTSAC CASTG^	5' - CASTG
NopI	SalI	GTCGAC	G^TCGAC CAGCT^G	5' - AGCT
NotI		GCGGCCGC	GC^GGCCGC CGCCGG^CG	5' - CCGG
NphI	MboI	GATC	^GATC CTAG^	5' - CTAG
NruI		TCGCGA	TCG^CGA AGC^GCT	Blunt
NruGI	Eam1105I	GACNNNNNGTC	GACNNN^NNGTC CTGNN^NNNCAG	N - 3'
NsbI	MstI	TGCGCA	TGC^GCA ACG^CGT	Blunt
NsiI	AvaIII	ATGCAT	ATGCA^T T^ACGTA	TGCA - 3'
NsiCI	EcoRV	GATATC	GAT^ATC CTA^TAG	Blunt
NspI		RCATGY	RCATG^Y R^GTACY	CATG - 3'
NspII	SduI	GDGCHC	GDGCH^C C^DCGHG	DGCH - 3'
NspIII	AvaI	CYCGRG	C^YCGRG GYGCR^C	5' - YGCR
NspIV	AsuI	GGNCC	G^GNCC CCNG^G	5' - CNG
NspV	AsuII	TTCGAA	TT^CGAA AAGC^TT	5' - GC
Nsp7121I	AsuI	GGNCC	G^GNCC CCNG^G	5' - CNG
Nsp29132II	BamHI	GGATCC	G^GATCC CCTAG^G	5' - CTAG
NspBII		CMGCKG	CMG^CKG GMC^GKC	Blunt
NspHI	NspI	RCATGY	RCATG^Y R^GTACY	CATG - 3'
NspLKI	HaeIII	GGCC	GG^CC CC^GG	Blunt
NspMACI	BglII	AGATCT	A^GATCT TCTAG^A	5' - CTAG
NspSAI	AvaI	CYCGRG	C^YCGRG GYGCR^C	5' - YGCR
NspSAII	BstEII	GGTNACC	G^GTNACC CCANTG^G	5' - CANTG
NspSAIV	BamHI	GGATCC	G^GATCC CCTAG^G	5' - CTAG
NunII	NarI	GGCGCC	GG^CGCC CCGC^GG	5' - GC
NmeAIII		GCCGAG	GCCGAGNNNNNNNNNNNNNNNNNNNN^ CGGCTCNNNNNNNNNNNNNNNNNN^NN	NN - 3'
OfoI	AvaI	CYCGRG	C^YCGRG GYGCR^C	5' - YGCR
OkrAI	BamHI	GGATCC	G^GATCC CCTAG^G	5' - CTAG
OliI		CACNNNNGTG	CACNN^NNGTG GTGNN^NNCAC	Blunt
OxaNI	SauI	CCTNAGG	CC^TNAGG GGANT^CC	5' - ANT
PacI		TTAATTAA	TTAAT^TAA AAT^TAATT	AT - 3'
Pac25I	SmaI	CCCGGG	C^CCGGG GGGCC^C	5' - GGCC
PaeI	SphI	GCATGC	GCATG^C C^GTACG	CATG - 3'
PaeAI	SacII	CCGCGG	CCGC^GG GG^CGCC	GC - 3'
PaeBI	SmaI	CCCGGG	CCC^GGG GGG^CCC	Blunt
PaeHI	HgiJII	GRGCYC	GRGCY^C C^RCGYG	RGCY - 3'
PaePI	PstI	CTGCAG	CTGCA^G G^ACGTC	TGCA - 3'
PaeQI	SacII	CCGCGG	CCGC^GG GG^CGCC	GC - 3'
PaeR7I	XhoI	CTCGAG	C^TCGAG GAGCT^C	5' - AGCT
Pae2kI	BglII	AGATCT	A^GATCT TCTAG^A	5' - CTAG
Pae5kI	SacII	CCGCGG	CCGC^GG GG^CGCC	GC - 3'
Pae14kI	SacII	CCGCGG	CCGC^GG GG^CGCC	GC - 3'
Pae17kI	PvuII	CAGCTG	CAG^CTG GTC^GAC	Blunt
Pae18kI	BglII	AGATCT	A^GATCT TCTAG^A	5' - CTAG
PagI	BspHI	TCATGA	T^CATGA AGTAC^T	5' - GTAC
PalI	HaeIII	GGCC	GG^CC CC^GG	Blunt
PamI	MstI	TGCGCA	TGC^GCA ACG^CGT	Blunt
PamII	AcyI	GRCGYC	GR^CGYC CRGC^YG	5' - GC
PanI	XhoI	CTCGAG	C^TCGAG GAGCT^C	5' - AGCT
PasI		CCCWGGG	CC^CWGGG GGGWC^CC	5' - GWC
PauI	BsePI	GCGCGC	G^CGCGC CGCGC^G	5' - GCGC
PauAI	NspI	RCATGY	RCATG^Y R^GTACY	CATG - 3'
PauAII	AhaIII	TTTAAA	TTT^AAA AAA^TTT	Blunt
PceI	StuI	AGGCCT	AGG^CCT TCC^GGA	Blunt
PciI	BspLU11I	ACATGT	A^CATGT TGTAC^A	5' - GTAC
PctI	BsmI	GAATGC	GAATGCN^ CTTAC^GN	CN - 3'
Pde12I	AsuI	GGNCC	G^GNCC CCNG^G	5' - CNG
Pde133I	HaeIII	GGCC	GG^CC CC^GG	Blunt
Pde137I	HpaII	CCGG	C^CGG GGC^C	5' - GC
PdiI	NaeI	GCCGGC	GCC^GGC CGG^CCG	Blunt
PdmI	XmnI	GAANNNNTTC	GAANN^NNTTC CTTNN^NNAAG	Blunt
PfaAI	HgiCI	GGYRCC	G^GYRCC CCYRG^G	5' - CYRG
PfaAII	NdeI	CATATG	CA^TATG GTAT^AC	5' - AT
PfaAIII	SphI	GCATGC	GCATG^C C^GTACG	CATG - 3'
PfeI	TfiI	GAWTC	G^AWTC CTWA^G	5' - TWA
Pfl8I	BamHI	GGATCC	G^GATCC CCTAG^G	5' - CTAG
Pfl21I	PstI	CTGCAG	CTGCA^G G^ACGTC	TGCA - 3'
Pfl23II	SplI	CGTACG	C^GTACG GCATG^C	5' - CATG
Pfl27I	PpuMI	RGGWCCY	RG^GWCCY RCCWG^GY	5' - CWG
PflBI	PflMI	CCANNNNNTGG	CCANNNN^NTGG GGTN^NNNNACC	NNN - 3'
PflFI	Tth111I	GACNNNGTC	GACN^NNGTC CTGNN^NCAG	5' - N
PflKI	HaeIII	GGCC	GG^CC CC^GG	Blunt
PflMI		CCANNNNNTGG	CCANNNN^NTGG GGTN^NNNNACC	NNN - 3'
PfoI		TCCNGGA	T^CCNGGA AGGNCC^T	5' - GGNCC
PgaI	ClaI	ATCGAT	AT^CGAT TAGC^TA	5' - GC
PhaI	SfaNI	GCATC	GCATCNNNNN^NNNN CGTAGNNNNNNNNN^	5' - NNNN
PhoI	HaeIII	GGCC	GG^CC CC^GG	Blunt
PinAI	AgeI	ACCGGT	A^CCGGT TGGCC^A	5' - GGCC
PinBI	AvaIII	ATGCAT	ATGCA^T T^ACGTA	TGCA - 3'
PinBII	BspMII	TCCGGA	T^CCGGA AGGCC^T	5' - GGCC
PlaI	HaeIII	GGCC	GG^CC CC^GG	Blunt
PlaII	AsuII	TTCGAA	TT^CGAA AAGC^TT	5' - GC
PlaAI	AvaI	CYCGRG	C^YCGRG GYGCR^C	5' - YGCR
PlaAII	RsaI	GTAC	GT^AC CA^TG	Blunt
PleI		GAGTC	GAGTCNNNN^N CTCAGNNNNN^	5' - N
Ple19I	PvuI	CGATCG	CGAT^CG GC^TAGC	AT - 3'
PmaCI		CACGTG	CAC^GTG GTG^CAC	Blunt
PmeI		GTTTAAAC	GTTT^AAAC CAAA^TTTG	Blunt
Pme55I	StuI	AGGCCT	AGG^CCT TCC^GGA	Blunt
PmlI	PmaCI	CACGTG	CAC^GTG GTG^CAC	Blunt
PpaAI	AsuII	TTCGAA	TT^CGAA AAGC^TT	5' - GC
PpaAII	TaqI	TCGA	T^CGA AGC^T	5' - GC
PpeI	ApaI	GGGCCC	GGGCC^C C^CCGGG	GGCC - 3'
PpiI		GAACNNNNNCTC	GAACNNNNNCTCNNNNNNNNNNNNN^ CTTGNNNNNGAGNNNNNNNN^NNNNN	NNNNN - 3'
PpsI	PleI	GAGTC	GAGTCNNNN^N CTCAGNNNNN^	5' - N
Ppu10I	AvaIII	ATGCAT	A^TGCAT TACGT^A	5' - ACGT
Ppu111I	EcoRI	GAATTC	G^AATTC CTTAA^G	5' - TTAA
PpuAI	SplI	CGTACG	C^GTACG GCATG^C	5' - CATG
PpuMI		RGGWCCY	RG^GWCCY RCCWG^GY	5' - CWG
PpuXI	PpuMI	RGGWCCY	RG^GWCCY RCCWG^GY	5' - CWG
PshAI		GACNNNNGTC	GACNN^NNGTC CTGNN^NNCAG	Blunt
PshBI	VspI	ATTAAT	AT^TAAT TAAT^TA	5' - AT
PsiI		TTATAA	TTA^TAA AAT^ATT	Blunt
Psp03I	AvaII	GGWCC	GGWC^C C^CWGG	GWC - 3'
Psp5II	PpuMI	RGGWCCY	RG^GWCCY RCCWG^GY	5' - CWG
Psp6I	EcoRII	CCWGG	^CCWGG GGWCC^	5' - GGWCC
Psp23I	PstI	CTGCAG	CTGCA^G G^ACGTC	TGCA - 3'
Psp1406I	AclI	AACGTT	AA^CGTT TTGC^AA	5' - GC
PspAI	SmaI	CCCGGG	C^CCGGG GGGCC^C	5' - GGCC
PspALI	SmaI	CCCGGG	CCC^GGG GGG^CCC	Blunt
Psp124BI	SacI	GAGCTC	GAGCT^C C^TCGAG	AGCT - 3'
PspCI	PmaCI	CACGTG	CAC^GTG GTG^CAC	Blunt
PspEI	BstEII	GGTNACC	G^GTNACC CCANTG^G	5' - CANTG
PspGI	EcoRII	CCWGG	^CCWGG GGWCC^	5' - GGWCC
PspLI	SplI	CGTACG	C^GTACG GCATG^C	5' - CATG
PspN4I	NlaIV	GGNNCC	GGN^NCC CCN^NGG	Blunt
PspOMI	ApaI	GGGCCC	G^GGCCC CCCGG^G	5' - CCGG
PspPI	AsuI	GGNCC	G^GNCC CCNG^G	5' - CNG
PspPPI	PpuMI	RGGWCCY	RG^GWCCY RCCWG^GY	5' - CWG
PspXI		VCTCGAGB	VC^TCGAGB VGAGCT^CB	5' - AGCT
PsrI		GAACNNNNNNTAC	GAACNNNNNNTACNNNNNNNNNNNN^ CTTGNNNNNNATGNNNNNNN^NNNNN	NNNNN - 3'
PssI	DraII	RGGNCCY	RGGNC^CY RC^CNGGY	GNC - 3'
PstI		CTGCAG	CTGCA^G G^ACGTC	TGCA - 3'
PstNHI	NheI	GCTAGC	G^CTAGC CGATC^G	5' - GATC
PsuI	XhoII	RGATCY	R^GATCY RCTAG^Y	5' - CTAG
Psu161I	PvuI	CGATCG	CGAT^CG GC^TAGC	AT - 3'
PsuAI	BsaAI	YACGTR	YAC^GTR YTG^CAR	Blunt
PsyI	Tth111I	GACNNNGTC	GACN^NNGTC CTGNN^NCAG	5' - N
PtaI	BspMII	TCCGGA	T^CCGGA AGGCC^T	5' - GGCC
Pun14627I	MstI	TGCGCA	TGC^GCA ACG^CGT	Blunt
Pun14627II	PvuII	CAGCTG	CAG^CTG GTC^GAC	Blunt
PunAI	AvaI	CYCGRG	C^YCGRG GYGCR^C	5' - YGCR
PunAII	NspI	RCATGY	RCATG^Y R^GTACY	CATG - 3'
PvuI		CGATCG	CGAT^CG GC^TAGC	AT - 3'
PvuII		CAGCTG	CAG^CTG GTC^GAC	Blunt
Pvu84II	PvuII	CAGCTG	CAG^CTG GTC^GAC	Blunt
PI-SceI		ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGGCA	ATCTATGTCGGGTGC^GGAGAAAGAGGTAATGAAATGGCA TAGATACAGCC^CACGCCTCTTTCTCCATTACTTTACCGT	GTGC - 3'
PalAI	AscI	GGCGCGCC	GG^CGCGCC CCGCGC^GG	5' - GCGC
PciSI	SapI	GCTCTTC	GCTCTTCN^NNN CGAGAAGNNNN^	5' - NNN
PI-PspI		TGGCAAACAGCTATTATGGGTATTATGGGT	TGGCAAACAGCTATTAT^GGGTATTATGGGT ACCGTTTGTCGAT^AATACCCATAATACCCA	TTAT - 3'
PluTI	NarI	GGCGCC	GGCGC^C C^CGCGG	GCGC - 3'
Ppu21I		YACGTR	YAC^GTR YTG^CAR	Blunt
PscI		ACATGT	A^CATGT TGTAC^A	5' - GTAC
PspFI		CCCAGC	CCCAG^C G^GGTCG	CCAG - 3'
RalF40I	MboI	GATC	^GATC CTAG^	5' - CTAG
RcaI	BspHI	TCATGA	T^CATGA AGTAC^T	5' - GTAC
RflFI	SalI	GTCGAC	G^TCGAC CAGCT^G	5' - AGCT
RflFII	ScaI	AGTACT	AGT^ACT TCA^TGA	Blunt
RleAI		CCCACA	CCCACANNNNNNNNNNNN^ GGGTGTNNNNNNNNN^NNN	NNN - 3'
RmaI	MaeI	CTAG	C^TAG GAT^C	5' - AT
Rme21I	ClaI	ATCGAT	AT^CGAT TAGC^TA	5' - GC
RsaI		GTAC	GT^AC CA^TG	Blunt
RshI	PvuI	CGATCG	CGAT^CG GC^TAGC	AT - 3'
RspLKI	SphI	GCATGC	GCATG^C C^GTACG	CATG - 3'
RspLKII	BamHI	GGATCC	G^GATCC CCTAG^G	5' - CTAG
RspXI	BspHI	TCATGA	T^CATGA AGTAC^T	5' - GTAC
RsrI	EcoRI	GAATTC	G^AATTC CTTAA^G	5' - TTAA
RsrII		CGGWCCG	CG^GWCCG GCCWG^GC	5' - CWG
Rsr2I	RsrII	CGGWCCG	CG^GWCCG GCCWG^GC	5' - CWG
RtrI	SalI	GTCGAC	G^TCGAC CAGCT^G	5' - AGCT
Rtr63I	SalI	GTCGAC	G^TCGAC CAGCT^G	5' - AGCT
RgaI		GCGATCGC	GCGAT^CGC CGC^TAGCG	AT - 3'
RigI		GGCCGGCC	GGCCGG^CC CC^GGCCGG	CCGG - 3'
RsaNI		GTAC	G^TAC CAT^G	5' - AT
RseI		CAYNNNNRTG	CAYNN^NNRTG GTYNN^NNRAC	Blunt
SacI		GAGCTC	GAGCT^C C^TCGAG	AGCT - 3'
SacII		CCGCGG	CCGC^GG GG^CGCC	GC - 3'
SacNI	HgiJII	GRGCYC	GRGCY^C C^RCGYG	RGCY - 3'
SalI		GTCGAC	G^TCGAC CAGCT^G	5' - AGCT
SalPI	PstI	CTGCAG	CTGCA^G G^ACGTC	TGCA - 3'
SanDI		GGGWCCC	GG^GWCCC CCCWG^GG	5' - CWG
SapI		GCTCTTC	GCTCTTCN^NNN CGAGAAGNNNN^	5' - NNN
SarI	StuI	AGGCCT	AGG^CCT TCC^GGA	Blunt
SatI	Fnu4HI	GCNGC	GC^NGC CGN^CG	5' - N
SauI		CCTNAGG	CC^TNAGG GGANT^CC	5' - ANT
Sau96I	AsuI	GGNCC	G^GNCC CCNG^G	5' - CNG
Sau3239I	XhoI	CTCGAG	C^TCGAG GAGCT^C	5' - AGCT
Sau3AI	MboI	GATC	^GATC CTAG^	5' - CTAG
SauBMKI	NaeI	GCCGGC	GCC^GGC CGG^CCG	Blunt
SauHPI	NaeI	GCCGGC	GCC^GGC CGG^CCG	Blunt
SauLPI	NaeI	GCCGGC	GCC^GGC CGG^CCG	Blunt
SauLPII	XhoI	CTCGAG	C^TCGAG GAGCT^C	5' - AGCT
SauMI	MboI	GATC	^GATC CTAG^	5' - CTAG
SauNI	NaeI	GCCGGC	GCC^GGC CGG^CCG	Blunt
SauSI	NaeI	GCCGGC	GCC^GGC CGG^CCG	Blunt
SbfI	Sse8387I	CCTGCAGG	CCTGCA^GG GG^ACGTCC	TGCA - 3'
Sbi68I	XhoI	CTCGAG	C^TCGAG GAGCT^C	5' - AGCT
Sbo13I	NruI	TCGCGA	TCG^CGA AGC^GCT	Blunt
SbvI	HaeIII	GGCC	GG^CC CC^GG	Blunt
ScaI		AGTACT	AGT^ACT TCA^TGA	Blunt
SceIII	NaeI	GCCGGC	G^CCGGC CGGCC^G	5' - GGCC
SchI	PleI	GAGTC	GAGTCNNNNN^ CTCAGNNNNN^	Blunt
SchZI	SacII	CCGCGG	CCGC^GG GG^CGCC	GC - 3'
SciI	XhoI	CTCGAG	CTC^GAG GAG^CTC	Blunt
SciNI	HhaI	GCGC	G^CGC CGC^G	5' - GC
ScrFI		CCNGG	CC^NGG GGN^CC	5' - N
SdaI	Sse8387I	CCTGCAGG	CCTGCA^GG GG^ACGTCC	TGCA - 3'
SdiI	SfiI	GGCCNNNNNGGCC	GGCCNNNN^NGGCC CCGGN^NNNNCCGG	NNN - 3'
SduI		GDGCHC	GDGCH^C C^DCGHG	DGCH - 3'
SecI		CCNNGG	C^CNNGG GGNNC^C	5' - GNNC
SelI	FnuDII	CGCG	^CGCG GCGC^	5' - GCGC
SenPT16I	XmaIII	CGGCCG	C^GGCCG GCCGG^C	5' - CCGG
SenPT14bI	SacII	CCGCGG	CCGC^GG GG^CGCC	GC - 3'
SepI	AvaIII	ATGCAT	ATGCA^T T^ACGTA	TGCA - 3'
SexAI		ACCWGGT	A^CCWGGT TGGWCC^A	5' - GGWCC
SexBI	SacII	CCGCGG	CCGC^GG GG^CGCC	GC - 3'
SexCI	SacII	CCGCGG	CCGC^GG GG^CGCC	GC - 3'
SfaI	HaeIII	GGCC	GG^CC CC^GG	Blunt
SfaNI		GCATC	GCATCNNNNN^NNNN CGTAGNNNNNNNNN^	5' - NNNN
SfcI	SfeI	CTRYAG	C^TRYAG GARYT^C	5' - ARYT
SfeI		CTRYAG	C^TRYAG GARYT^C	5' - ARYT
SfiI		GGCCNNNNNGGCC	GGCCNNNN^NGGCC CCGGN^NNNNCCGG	NNN - 3'
SflI	PstI	CTGCAG	CTGCA^G G^ACGTC	TGCA - 3'
SfoI	NarI	GGCGCC	GGC^GCC CCG^CGG	Blunt
Sfr274I	XhoI	CTCGAG	C^TCGAG GAGCT^C	5' - AGCT
Sfr303I	SacII	CCGCGG	CCGC^GG GG^CGCC	GC - 3'
SfuI	AsuII	TTCGAA	TT^CGAA AAGC^TT	5' - GC
SgfI		GCGATCGC	GCGAT^CGC CGC^TAGCG	AT - 3'
SgrAI		CRCCGGYG	CR^CCGGYG GRGGCC^YC	5' - GGCC
SgrBI	SacII	CCGCGG	CCGC^GG GG^CGCC	GC - 3'
SimI		GGGTC	GG^GTC CCCAG^	5' - CAG
SinI	AvaII	GGWCC	G^GWCC CCWG^G	5' - CWG
SlaI	XhoI	CTCGAG	C^TCGAG GAGCT^C	5' - AGCT
SleI	EcoRII	CCWGG	^CCWGG GGWCC^	5' - GGWCC
Slu1777I	NaeI	GCCGGC	GCC^GGC CGG^CCG	Blunt
SmaI		CCCGGG	CCC^GGG GGG^CCC	Blunt
SmiI	SwaI	ATTTAAAT	ATTT^AAAT TAAA^TTTA	Blunt
SmiMI	MslI	CAYNNNNRTG	CAYNN^NNRTG GTYNN^NNRAC	Blunt
SmlI		CTYRAG	C^TYRAG GAYRT^C	5' - AYRT
SmuI	FauI	CCCGC	CCCGCNNNN^NN GGGCGNNNNNN^	5' - NN
SmuEI	AvaII	GGWCC	G^GWCC CCWG^G	5' - CWG
SnaBI		TACGTA	TAC^GTA ATG^CAT	Blunt
SniI	EcoRII	CCWGG	CC^WGG GGW^CC	5' - W
SnoI	ApaLI	GTGCAC	G^TGCAC CACGT^G	5' - ACGT
SolI	BamHI	GGATCC	G^GATCC CCTAG^G	5' - CTAG
Sol10179I	XhoI	CTCGAG	C^TCGAG GAGCT^C	5' - AGCT
SpaHI	SphI	GCATGC	GCATG^C C^GTACG	CATG - 3'
SpeI		ACTAGT	A^CTAGT TGATC^A	5' - GATC
SphI		GCATGC	GCATG^C C^GTACG	CATG - 3'
SplI		CGTACG	C^GTACG GCATG^C	5' - CATG
SpmI	ClaI	ATCGAT	AT^CGAT TAGC^TA	5' - GC
SpoI	NruI	TCGCGA	TCG^CGA AGC^GCT	Blunt
SpuI	SacII	CCGCGG	CCGC^GG GG^CGCC	GC - 3'
SrfI		GCCCGGGC	GCCC^GGGC CGGG^CCCG	Blunt
SrlI	NaeI	GCCGGC	G^CCGGC CGGCC^G	5' - GGCC
Srl5DI	PstI	CTGCAG	CTGCA^G G^ACGTC	TGCA - 3'
Srl32DII	EcoRI	GAATTC	G^AATTC CTTAA^G	5' - TTAA
Srl55DI	EcoRI	GAATTC	G^AATTC CTTAA^G	5' - TTAA
Srl56DI	SfeI	CTRYAG	C^TRYAG GARYT^C	5' - ARYT
SruI	AhaIII	TTTAAA	TTT^AAA AAA^TTT	Blunt
Sru4DI	VspI	ATTAAT	AT^TAAT TAAT^TA	5' - AT
Sru30DI	StuI	AGGCCT	AGG^CCT TCC^GGA	Blunt
SsbI	HindIII	AAGCTT	A^AGCTT TTCGA^A	5' - TCGA
SscL1I	HinfI	GANTC	G^ANTC CTNA^G	5' - TNA
Sse9I	TspEI	AATT	^AATT TTAA^	5' - TTAA
Sse232I		CGCCGGCG	CG^CCGGCG GCGGCC^GC	5' - GGCC
Sse1825I	SanDI	GGGWCCC	GG^GWCCC CCCWG^GG	5' - CWG
Sse8387I		CCTGCAGG	CCTGCA^GG GG^ACGTCC	TGCA - 3'
Sse8647I		AGGWCCT	AG^GWCCT TCCWG^GA	5' - CWG
SseAI	NarI	GGCGCC	GG^CGCC CCGC^GG	5' - GC
SseBI	StuI	AGGCCT	AGG^CCT TCC^GGA	Blunt
SshAI	SauI	CCTNAGG	CC^TNAGG GGANT^CC	5' - ANT
SsiI	AciI	CCGC	C^CGC GGC^G	5' - GC
SsiAI	MboI	GATC	^GATC CTAG^	5' - CTAG
SsiBI	MboI	GATC	^GATC CTAG^	5' - CTAG
SslI	EcoRII	CCWGG	CC^WGG GGW^CC	5' - W
SsoI	EcoRI	GAATTC	G^AATTC CTTAA^G	5' - TTAA
SsoII	ScrFI	CCNGG	^CCNGG GGNCC^	5' - GGNCC
SspI		AATATT	AAT^ATT TTA^TAA	Blunt
Ssp1I	AsuII	TTCGAA	TT^CGAA AAGC^TT	5' - GC
Ssp4800I	Bsp1407I	TGTACA	T^GTACA ACATG^T	5' - CATG
Ssp5230I	AatII	GACGTC	GACGT^C C^TGCAG	ACGT - 3'
Ssp27144I	ClaI	ATCGAT	AT^CGAT TAGC^TA	5' - GC
SspAI	EcoRII	CCWGG	^CCWGG GGWCC^	5' - GGWCC
SspBI	Bsp1407I	TGTACA	T^GTACA ACATG^T	5' - CATG
SspCI	NaeI	GCCGGC	GCC^GGC CGG^CCG	Blunt
SspD5I	HphI	GGTGA	GGTGANNNNNNNN^ CCACTNNNNNNNN^	Blunt
SspD5II	AvaIII	ATGCAT	ATGCA^T T^ACGTA	TGCA - 3'
SspRFI	AsuII	TTCGAA	TT^CGAA AAGC^TT	5' - GC
SsrI	HpaI	GTTAAC	GTT^AAC CAA^TTG	Blunt
SstI	SacI	GAGCTC	GAGCT^C C^TCGAG	AGCT - 3'
SstII	SacII	CCGCGG	CCGC^GG GG^CGCC	GC - 3'
Sst12I	PstI	CTGCAG	CTGCA^G G^ACGTC	TGCA - 3'
SteI	StuI	AGGCCT	AGG^CCT TCC^GGA	Blunt
SthI	KpnI	GGTACC	G^GTACC CCATG^G	5' - CATG
Sth117I	EcoRII	CCWGG	CC^WGG GGW^CC	5' - W
Sth132I		CCCG	CCCGNNNN^NNNN GGGCNNNNNNNN^	5' - NNNN
Sth134I	HpaII	CCGG	C^CGG GGC^C	5' - GC
Sth368I	MboI	GATC	^GATC CTAG^	5' - CTAG
StrI	XhoI	CTCGAG	C^TCGAG GAGCT^C	5' - AGCT
StsI	FokI	GGATG	GGATGNNNNNNNNNN^NNNN CCTACNNNNNNNNNNNNNN^	5' - NNNN
StuI		AGGCCT	AGG^CCT TCC^GGA	Blunt
StyI		CCWWGG	C^CWWGG GGWWC^C	5' - GWWC
StyD4I	ScrFI	CCNGG	^CCNGG GGNCC^	5' - GGNCC
SuaI	HaeIII	GGCC	GG^CC CC^GG	Blunt
SunI	SplI	CGTACG	C^GTACG GCATG^C	5' - CATG
SurI	BamHI	GGATCC	G^GATCC CCTAG^G	5' - CTAG
SviI	AsuII	TTCGAA	TT^CGAA AAGC^TT	5' - GC
SwaI		ATTTAAAT	ATTT^AAAT TAAA^TTTA	Blunt
SetI		ASST	ASST^ ^TSSA	ASST - 3'
SfaAI		GCGATCGC	GCGAT^CGC CGC^TAGCG	AT - 3'
SgrDI		CGTCGACG	CG^TCGACG GCAGCT^GC	5' - AGCT
SgsI		GGCGCGCC	GG^CGCGCC CCGCGC^GG	5' - GCGC
SmoI		CTYRAG	C^TYRAG GAYRT^C	5' - AYRT
SspDI		GGCGCC	G^GCGCC CCGCG^G	5' - CGCG
TaaI	Tsp4CI	ACNGT	ACN^GT TG^NCA	N - 3'
TaiI	MaeII	ACGT	ACGT^ ^TGCA	ACGT - 3'
TaqI		TCGA	T^CGA AGC^T	5' - GC
TaqII		GACCGACACCCA	GACCGACACCCANNNNNNNNNNN^ CTGGCTGTGGGTNNNNNNNNN^NN	NN - 3'
Taq52I	TseI	GCWGC	G^CWGC CGWC^G	5' - GWC
TaqXI	EcoRII	CCWGG	CC^WGG GGW^CC	5' - W
TasI	TspEI	AATT	^AATT TTAA^	5' - TTAA
TatI		WGTACW	W^GTACW WCATG^W	5' - CATG
TauI		GCSGC	GCSG^C C^GSCG	CSG - 3'
TelI	Tth111I	GACNNNGTC	GACN^NNGTC CTGNN^NCAG	5' - N
TfiI		GAWTC	G^AWTC CTWA^G	5' - TWA
ThaI	FnuDII	CGCG	CG^CG GC^GC	Blunt
TliI	XhoI	CTCGAG	C^TCGAG GAGCT^C	5' - AGCT
Tru1I	MseI	TTAA	T^TAA AAT^T	5' - AT
Tru9I	MseI	TTAA	T^TAA AAT^T	5' - AT
Tru201I	XhoII	RGATCY	R^GATCY RCTAG^Y	5' - CTAG
TscI	MaeII	ACGT	ACGT^ ^TGCA	ACGT - 3'
TseI		GCWGC	G^CWGC CGWC^G	5' - GWC
Tsp1I	BsrI	ACTGG	ACTGGN^ TGAC^CN	GN - 3'
Tsp32I	TaqI	TCGA	T^CGA AGC^T	5' - GC
Tsp32II	TaqI	TCGA	T^CGA AGC^T	5' - GC
Tsp45I		GTSAC	^GTSAC CASTG^	5' - CASTG
Tsp49I	MaeII	ACGT	ACGT^ ^TGCA	ACGT - 3'
Tsp509I	TspEI	AATT	^AATT TTAA^	5' - TTAA
TspBI	DsaI	CCRYGG	C^CRYGG GGRYC^C	5' - GRYC
Tsp4CI		ACNGT	ACN^GT TG^NCA	N - 3'
TspDTI		ATGAA	ATGAANNNNNNNNNNN^ TACTTNNNNNNNNN^NN	NN - 3'
TspEI		AATT	^AATT TTAA^	5' - TTAA
Tsp8EI	BglI	GCCNNNNNGGC	GCCNNNN^NGGC CGGN^NNNNCCG	NNN - 3'
TspGWI		ACGGA	ACGGANNNNNNNNNNN^ TGCCTNNNNNNNNN^NN	NN - 3'
TspRI		CASTGNN	CASTGNN^ ^GTSACNN	CASTGNN - 3'
Tth111I		GACNNNGTC	GACN^NNGTC CTGNN^NCAG	5' - N
Tth111II		CAARCA	CAARCANNNNNNNNNNN^ GTTRGTNNNNNNNNN^NN	NN - 3'
TthHB8I	TaqI	TCGA	T^CGA AGC^T	5' - GC
TscAI		NNCASTGNN	NNCASTGNN^ ^NNGTSACNN	NNCASTGNN - 3'
TsoI		TARCCA(N)9NN	TARCCA(N)9NNNNNNN^ ATRGGT(N)9NNNNN^NN	NN - 3'
TspMI	Sma I	CCCGGG	C^CCGGG GGGCC^C	5' - GGCC
Uba4009I	BamHI	GGATCC	G^GATCC CCTAG^G	5' - CTAG
Uba153AI	PvuII	CAGCTG	CAG^CTG GTC^GAC	Blunt
UbaM39I	PvuII	CAGCTG	CAG^CTG GTC^GAC	Blunt
UnbI	AsuI	GGNCC	^GGNCC CCNGG^	5' - CCNGG
UthSI	SmaI	CCCGGG	CC^CGGG GGGC^CC	5' - GC
Uur960I	Fnu4HI	GCNGC	GC^NGC CGN^CG	5' - N
Van91I	PflMI	CCANNNNNTGG	CCANNNN^NTGG GGTN^NNNNACC	NNN - 3'
Vha464I	AflII	CTTAAG	C^TTAAG GAATT^C	5' - AATT
VneI	ApaLI	GTGCAC	G^TGCAC CACGT^G	5' - ACGT
VpaK32I	SapI	GCTCTTC	GCTCTTCN^NNN CGAGAAGNNNN^	5' - NNN
VpaK11AI	AvaII	GGWCC	^GGWCC CCWGG^	5' - CCWGG
VpaK11BI	AvaII	GGWCC	G^GWCC CCWG^G	5' - CWG
VspI		ATTAAT	AT^TAAT TAAT^TA	5' - AT
XagI	EcoNI	CCTNNNNNAGG	CCTNN^NNNAGG GGANNN^NNTCC	5' - N
XapI	ApoI	RAATTY	R^AATTY RTTAA^Y	5' - TTAA
XbaI		TCTAGA	T^CTAGA AGATC^T	5' - GATC
XcaI	SnaI	GTATAC	GTA^TAC CAT^ATG	Blunt
XceI	NspI	RCATGY	RCATG^Y R^GTACY	CATG - 3'
XciI	SalI	GTCGAC	G^TCGAC CAGCT^G	5' - AGCT
XcmI		CCANNNNNNNNNTGG	CCANNNNN^NNNNTGG GGTNNNN^NNNNNACC	N - 3'
XcyI	SmaI	CCCGGG	C^CCGGG GGGCC^C	5' - GGCC
XhoI		CTCGAG	C^TCGAG GAGCT^C	5' - AGCT
XhoII		RGATCY	R^GATCY RCTAG^Y	5' - CTAG
XmaI	SmaI	CCCGGG	C^CCGGG GGGCC^C	5' - GGCC
XmaIII		CGGCCG	C^GGCCG GCCGG^C	5' - CCGG
XmaCI	SmaI	CCCGGG	C^CCGGG GGGCC^C	5' - GGCC
XmaJI	AvrII	CCTAGG	C^CTAGG GGATC^C	5' - GATC
XmiI	AccI	GTMKAC	GT^MKAC CAMK^TG	5' - MK
XmnI		GAANNNNTTC	GAANN^NNTTC CTTNN^NNAAG	Blunt
XorII	PvuI	CGATCG	CGAT^CG GC^TAGC	AT - 3'
XpaI	XhoI	CTCGAG	C^TCGAG GAGCT^C	5' - AGCT
XspI	MaeI	CTAG	C^TAG GAT^C	5' - AT
YenI	PstI	CTGCAG	CTGCA^G G^ACGTC	TGCA - 3'
ZanI	EcoRII	CCWGG	CC^WGG GGW^CC	5' - W
ZhoI	ClaI	ATCGAT	AT^CGAT TAGC^TA	5' - GC
ZraI	AatII	GACGTC	GAC^GTC CTG^CAG	Blunt
ZrmI	ScaI	AGTACT	AGT^ACT TCA^TGA	Blunt
Zsp2I	AvaIII	ATGCAT	ATGCA^T T^ACGTA	TGCA - 3'

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