Together with our experienced scientific team, Creative Biostructure has established a comprehensive platform to provide custom Mempro™ Virus-like Particles (VLPs) production services. We are expertized in virus-like particles research area for years, and we assure to offer you the high-quality products and the best services.
VLPs can mimic the whole structure of virus particles while devoid of the viral genome and induce protective immunity efﬁciently as direct immunogens. The proper selection of the expression systems plays an important role in VLPs production. Creative Biostructure has developed a broad range of expression systems to produce VLPs with proper post-translational modifications and assembly, and Mempro™ Virus-like Particles (VLPs) production in plant cells system is broadly applied.
Table 1. Plant-produced vLP-based vaccines. (Human vaccines & Immunotherapeutics, 2013)
Plant cells system is another attractive system especially aiming at VLPs production because they are able to perform large scale production at low cost, decrease the risk of introducing human pathogens. And do not need expensive fermentation facilities to generate biomass. Creative Biostructure has already successfully produced custom VLPs products using tobacco plants, potato tubers, and other plant. This expression system can be delivered orally with a normal biologic function in human. The antigen expressed in plant cells system shows extensive oligomerization for formation of VLPs. Furthermore, plant cells can express both enveloped VLPs and non-enveloped VLPs. Creative Biostructure can also apply plant virus particles to present foreign epitopes as a delivery system. The major limitation of this expression system is the relative low expression level. To overcome this problem, we have developed several novel plant-expression vectors based on “deconstructed” plant viruses to enhance the expression level which can compete with mammalian cells system.
Bacterial cells system, yeast cells system, mammalian cells system, and plant cells system provided by Creative Biostructure are the alternative expression systems for VLPs production. Beyond VLPs production, our Mempro™ virus-like particles (VLPs) platform also provide services focused on the application, characterization and functionalization strategies of custom VLPs. Please feel free to contact us for a detailed quote.
Q. Chen, et al. (2013). Plant-derived virus-like particles as vaccines. Human vaccines & Immunotherapeutics, 9(1): 26-49.
Y. Gleba, et al. (2007). Viral vectors for the expression of proteins in plants. Current Opinion in Biotechnology, 18, 134-141.
H. Lai, and Q. Chen. (2012). Bioprocessing of plant-derived virus-like particles of Norwalk virus capsid protein under current Good Manufacture Practice regulations. Plant Cell Reports, 31(3): 573–584.