Mempro™ Nanodisc Application

Creative Biostructure has focused on the development of the nanodisc technology for years and established a magic Mempro™ Nanodisc platform to satisfy all your custom demands through the project. Together with our advanced Mempro™ Membrane Protein platform, we have applied Mempro™ Nanodisc technology in a series of fields to study membrane proteins as follows:

Figure 1. An improved antibody phage display protocol for membrane proteins exploiting nanodiscs, expanding the usability of the method to multiple challenging membrane protein systems. (Structure, 2016)

• Mempro™ Nanodisc for Membrane Protein Purification

Creative Biostructure is expertized in membrane protein production and purification. It is well known that in order for some membrane proteins to function correctly, a specific lipid environment has to be present. Nanodiscs have proven to be an excellent membrane mimetic and solution to the difficulties of working with membrane proteins. Mempro™ Nanodisc technology can provide a native-like environment for isolating membrane proteins based on our cell-free production system.

• Mempro™ Nanodisc for Structural Investigation of Membrane Proteins

Membrane proteins are especially difficult to crystallize since the lipid bilayer environment stabilizes their conformation. The 3D structure of most water-soluble proteins may be solved using NMR spectrometry with high-molecular mass being a limiting factor. Mempro™ Nanodisc technology offers a perfect water-soluble lipid membrane system for structural investigation of membrane proteins by the means of NMR.

• Mempro™ Nanodisc as Model Membranes

Mempro™ Nanodisc has proven to be powerful model membranes with valuable information about membrane biophysics. Phase transition studies of our nanodiscs comprised of DPPC and DMPC have shown a broader phase transition and a higher temperature than liposomes by using differential scanning calorimetry and laurdan fluorescence, which means that fewer lipids are required for phase transitions in nanodiscs than in liposomes.

• Mempro™ Nanodisc for Receptor Studies

It is very important for a better understanding of the oligomerization of numerous integral membrane proteins including various receptors such as GPCRs. Mempro™ Nanodisc technology has been successfully applied in receptor production with different oligomeric states and the influence analysis of receptor oligomerization.

• Mempro™ Nanodisc for Ligand-binding Studies

Investigating the binding between potential drug candidates and target membrane protein receptors is of great importance for drug development. Mempro™ Nanodisc technology can incorporate the membrane-anchored CYPs into nanodiscs as a potential tool to obtain these membrane proteins in a functionally active, soluble, and monodisperse form. We can apply localized surface plasmon resonance to perform ligand-binding studies.

Creative Biostructure also provides a wide range of Mempro™ Nanodisc products and services. Please feel free to contact us for a detailed quote.

References:
P. K. Dominik, et al. (2016). Conformational Chaperones for Structural Studies of Membrane Proteins Using Antibody Phage Display with Nanodiscs. Structure,24: 300–309.
I. G. Denisov and S. G. Sligar. (2016). Nanodiscs for structural and functional studies of membrane proteins. Nature Structural & Molecular Biology,23: 481–486.
P. Hörnschemeyer, et al. (2016). Interaction Analysis of a Two-Component System Using Nanodiscs. Plos ONE, 11(2): e0149187.