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Product Summary
Product Overview
The fluorescent control liposomes contain the green lipophilic dye DiO incorporated in the bilayers. It is used for tracking the cellular uptake of the liposomes by common methods like confocal, FACS, etc. The green fluorescent, lipophilic carbocyanine DiOC18(3) is widely used as a lipophilic tracer. It is weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes. It has an extremely high extinction coefficient and short excited-state lifetimes (~1 nanosecond) in lipid environments.<br />Lipid composition: DOPC/CHOL (54:45 mol/mol)<br />Mean particle size: 100 nm (90-120 nm)
Lipid Composition
DOPC; CHOL
Application
Liposome production; Synthetic lipid
Storage Buffer
Hydration buffer: 10% sucrose, 20mM HEPES, pH 7.3 ± 0.2
Concentration
Lipid concentration: 50 mM (50-55 mM)<br />Dye: 0.5 mM (0.44 mg/mL)
Stability
6 Month for unopened vials.
Storage
Store at 2-8 centigrade.
Synonyms
DOPC; 1,2-dioleoyl-sn-glycero-3-phosphocholine; CHOL; cholesterol