Product Overview
The liposomes are prepared by the extrusion technology to 200-250nm nm with tight particle size distribution. The product is semisterile filtered through 0.45um filters and filled in autoclaved vials guaranteed to be stable for 3 months stored in refrigerator. The phospholipid, fluorescein DHPE is labeled on the head group with the green-fluorescent pH-sensitive fluorophore, fluorescein (excitation/emission maxima ~496/519 nm). <br />Lipid composition: DOPC/CHOL/Fluorescein-DHPE (54:45:1 mol/mol)<br />Mean particle size: 230 nm (200-250 nm, intensity weighted lognormal per DSL)<br />Particle size distribution: 80-100nm (half-width)
Application
Liposome production; Synthetic lipid
Storage Buffer
Hydration buffer: 10% sucrose, 20mM HEPES, pH 7.5 ± 0.2
Concentration
Lipid concentration: 50 mM (50-55 mM)<br />Fluorscein DHPE: 0.5 mM (0.59 mg/mL)
Stability
6 Month for unopened vials.
Storage
Store at 2-8 centigrade, protect from light.
Synonyms
DOPC; 1,2-dioleoyl-sn-glycero-3-phosphocholine; CHOL; cholesterol