Product Overview
The liposomes are prepared by the extrusion method to 300nm with tight particle size distribution. The product is semisterile filtered through 0.45um filters and filled in autoclaved vials guaranteed to be stable for 3 months stored in refrigerator. The phospholipid, fluorescein DHPE is labeled on the head group with the green-fluorescent pH-sensitive fluorophore, fluorescein (excitation/emission maxima ~496/519 nm). <br />Lipid composition: DOPC/CHOL/F-DHPE (54:45:1 mol/mol)<br />Mean particle size: 280 nm (250-320 nm)
Application
Liposome production; Synthetic lipid
Storage Buffer
Hydration buffer: 10% sucrose, 20mM HEPES, pH 7.5 ± 0.2
Concentration
Lipid concentration: 50 mM (50-55 mM)<br />Fluorscein DHPE: 0.5 mM (0.59 mg/mL)
Stability
3 Month for unopened vials.
Synonyms
DOPC; 1,2-dioleoyl-sn-glycero-3-phosphocholine; CHOL; cholesterol