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Product Summary
Product Overview
pHrodo Red is a pH sensitive dye which increases in fluorescence as pH decreases from more neutral to acidic. Dextran-pHrodo Red conjugated liposomes are commonly used for detecting endocytosis and phagocytosis, respectively, as the dyes display little to no fluorescence outside the cell at neutral pH and upon internalization, emit a bright red or green signal due to the acidic environment of endosomes and phagosomes. Excitation/Emission (nm): 560/585
Lipid Composition
18:1 (?9-Cis) PG/DOPC/CHOL(2.0/5.0/3.0 mM) 18:1 (?9-Cis) PG: 1,2-dioleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (sodium salt)/CAS: 67254-28-8 DOPC: 1,2-dioleoyl-sn-glycero-3-phosphocholine/CAS: 4235-95-4 CHOL: Cholesterol/CAS: 57-88-5
Form
Liquid
Storage Buffer
20 mM HEPES, pH 7.4
Concentration
Lipid Concentration 10mM
Stability
2 months
Storage
2 °C to 8 °C (do not freeze)