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Product Summary
Product Overview
Cationic liposomes are traditionally used for the delivery of genetic materials such as various types of DNA (pDNA, cDNA, CpG DNA, oligonucleotide, antisense oligonucleotide, etc.), various types of RNA such as (siRNA, mRNA, etc.) and nucleic acid mimics (NAMs). DOTAP is a kind of lipid which has one positive charge per MOLECULE. Using DOTAP in liposome can neutralize the negative charge of pDNA with positive charge of cationic lipids in order to capture more plasmid efficiently mainly due to electrostatic interaction and deliver them into the cells.
Lipid Composition
DOTAP/DOPE(2/2 mM) 1,2-dioleoyl-3-trimethylammonium-propane (chloride salt (DOTAP) 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE)
Form
Liquid
Storage Buffer
Deionized RNAse-free Water, pH 7
Concentration
Lipid Concentration 4mM
Stability
2 months
Storage
2 °C to 8 °C (do not freeze)