Animal Tissue Exosome Isolation Service

Q: Q1: What is the typical yield and purity of tissue-derived exosomes?

Yield and purity depend on tissue type, sample condition, and isolation method. Our optimized protocol ensures nanometer-sized vesicles with confirmed exosomal markers and minimal protein contamination.

Q: Q2: Can I combine tissue- and fluid-derived exosome analysis?

Yes. We offer integrated platforms to trace tissue exosomes into systemic circulation, providing insight into exosomal transport pathways.

Q: Q3: What tissues are compatible with your platform?

Most soft tissues from mouse, rat, bovine, and porcine models are supported. Contact us to discuss specific compatibility and sample prep requirements.

Q: Q4: How much tissue is required for exosome isolation?

We typically recommend at least 2 grams of tissue for basic exosome isolation and characterization. For omics-based studies (e.g., RNA-seq or proteomics), larger input amounts (3-5 grams or more) may be needed to ensure sufficient yield and data quality. We'll advise based on your specific application.

Q: Q5: Can I request functional or omics analysis of the isolated exosomes?

Yes. We offer a full suite of downstream services including miRNA/mRNA sequencing, proteomics, lipidomics, and cell-based functional assays. These can be added to your project or contracted separately.

At Creative Biostructure, we offer a professional exosome isolation service from animal tissues to support cutting-edge research in intercellular communication, disease progression, and biomarker discovery. Compared to exosomes derived from cell culture or body fluids, tissue-derived exosomes preserve the molecular signatures of the native tissue microenvironment, offering unique biological insights with higher specificity.

Why Tissue-Derived Exosomes Matter

Body fluids like blood and urine contain a mixture of exosomes originating from multiple organs, which can dilute or obscure the signal from a specific tissue. In contrast, exosomes isolated directly from tissue interstitial spaces more accurately reflect localized cellular states, making them ideal for:

Disease mechanism studies
Diagnostic biomarker screening
Functional exosome profiling
Preclinical drug evaluation

Numerous studies have confirmed the physiological and pathological relevance of exosomes from adipose, brain, liver, and other tissues. Adipose tissue-derived exosomes (AT-exosomes), for instance, have shown involvement in metabolic regulation, neuroregeneration, and tumorigenesis.

Adipose tissue-derived exosomes regulate ATM polarization and adipogenesis in adipose tissue, liver, and muscle via ER-stress modulation.

Figure 1. Role of Adipose Tissue-Derived Exosomes in Metabolic Regulation. (Zhao R, et al., 2021)

Our Exosome Isolation Techniques

Creative Biostructure employs a range of advanced exosome isolation techniques to accommodate different sample types, purity requirements, and downstream applications. The table below summarizes the core technologies we use, along with their descriptions and suitable research contexts:

Technique	Description	Recommended Applications
Differential Ultracentrifugation	Sequential high-speed spins to isolate exosomes based on size and density.	Standard for high-throughput isolation from tissue homogenates and culture media.
Density Gradient Ultracentrifugation	Uses sucrose or iodixanol layers to achieve high-purity separation.	Best for functional assays, electron microscopy, and omics analysis.
Size-Exclusion Chromatography (SEC)	Separates vesicles by size through a porous column, preserving vesicle integrity.	Ideal for downstream RNA sequencing, proteomics, and functional exosome research.
Ultrafiltration (UF)	Uses membranes with specific molecular weight cut-offs to concentrate exosomes.	Suitable for rapid sample concentration and pre-purification prior to SEC or affinity.
Polymer-Based Precipitation	Uses hydrophilic polymers to precipitate exosomes from biological fluids.	Convenient for high-yield recovery from large sample volumes; suitable for screening.
Immunoaffinity Capture	Uses antibodies targeting surface markers (e.g., CD63, CD81) for specific capture.	High-specificity isolation for biomarker studies and targeted subpopulation analysis.

We also offer integrated isolation workflows that combine multiple techniques to optimize yield, purity, and functional integrity based on your specific research needs.

Our Tissue Exosome Isolation Workflow

Isolating exosomes from animal tissues requires careful handling to preserve vesicle integrity while eliminating contaminants. At Creative Biostructure, we have established a robust and customizable workflow optimized for various tissue types and downstream applications. The key steps include:

1

Tissue Dissection & Preprocessing

Animal tissues are finely minced or sectioned to maximize surface area. Depending on tissue type, optional perfusion or enzymatic softening is applied to improve exosome release.

2

Tissue Digestion & Conditioned Media Preparation

Samples are incubated with specialized enzyme cocktails (e.g., collagenase, DNase) in exosome-compatible medium to facilitate exosome diffusion from the interstitial space into the supernatant.

3

Preliminary Clarification

Sequential centrifugation at low to medium speeds (e.g., 300 ×g, 2,000 ×g, 10,000 ×g) removes cells, debris, apoptotic bodies, and larger vesicles.

4

Exosome Enrichment

We apply advanced isolation methods such as ultracentrifugation, size-exclusion chromatography (SEC), or immunoaffinity capture to selectively enrich for exosomes while preserving their biological activity.

5

Buffer Exchange & Concentration

Final concentration and buffer exchange are performed using ultrafiltration or dialysis to prepare exosomes for intended downstream assays (e.g., proteomics, sequencing, cell-based assays).

This modular workflow can be adapted based on tissue source, project goals, and preferred analytical endpoints. Additionally, we offer combined technology strategies to enhance both recovery and specificity, especially for low-yield or complex tissues.

Workflow for isolating exosomes from tissues, including processing, isolation, buffer exchange, and optional QC.

Figure 2. Tissue Exosome Isolation Project Workflow. (Creative Biostructure)

Supported Sample Types

We accept both fresh and frozen tissue specimens from multiple species, including:

Mouse (liver, brain, adipose, spleen)
Rat (kidney, skeletal muscle)
Bovine and porcine tissues
Other custom sources upon request

For optimal yield and QC performance, we recommend ≥2 grams of tissue per sample. Contact us for guidance on your specific tissue type.

Quality Control and Deliverables

Every exosome batch undergoes comprehensive quality testing, including:

Particle Size and Concentration by Nanoparticle Tracking Analysis (NTA)
Morphology via Transmission Electron Microscopy (TEM)
Exosomal Marker Validation (CD9, CD63, TSG101, Alix) using Western Blot or ELISA
Optional: RNA profiling, proteomics, and lipidomics upon request

You will receive:

Purified tissue-derived exosomes (lyophilized or in solution)
Technical documentation (detailed protocols, reagent information, sample handling notes, and storage guidelines)
Comprehensive QC report (NTA graphs, TEM images, marker validation data, and recommended downstream applications)
Optional omics datasets and functional assay results

Optional Add-On Services

To help you extract maximum biological insight from your exosome samples, Creative Biostructure offers a suite of optional downstream services tailored to diverse research goals. These add-on services can be bundled with our tissue exosome isolation workflow or requested independently.

Advanced Omics Analysis	Functional & Validation Assays	Customization & Engineering
Exosomal RNA Sequencing Deep profiling of miRNA, lncRNA, circRNA, and mRNA cargo for mechanistic studies and biomarker discovery. Exosome Proteomics Quantitative proteome mapping of exosomal contents using LC-MS/MS, useful for signaling pathway and biomarker analysis. Exosome Lipidomics & Metabolomics Comprehensive profiling of lipid and metabolite components to study energy regulation, inflammation, and metabolic disease pathways.	In Vitro Functional Assays Assess effects of tissue-derived exosomes on recipient cell phenotypes, including proliferation, migration, angiogenesis, and immune modulation· In Vivo Delivery & Tracking Optional animal-based studies to examine biodistribution, uptake, and physiological function of labeled exosomes. Co-Analysis of Tissue and Fluid-Derived Exosomes Enables tracing of tissue-specific exosomes into systemic circulation for translational research applications.	Surface Marker Enrichment Immuno-isolation of exosomal subpopulations based on tissue-specific or disease-related surface markers. Fluorescent/Radiolabeling Services For imaging, tracing, or quantitative biodistribution analysis in live-cell or in vivo models.

Each optional service is backed by expert consultation to ensure methodological compatibility and data reproducibility across platforms.

Frequently Asked Questions

For any inquiries, our support team is ready to help you get technical support for your research and maximize your experience with Creative Biostructure.

Case Study

Case: Tissue-Derived Exosome Isolation Enables Biomarker Discovery in Esophageal Cancer

Background

To identify novel biomarkers for esophageal cancer (EC), researchers developed a workflow for isolating exosomes directly from tumor and adjacent tissues, offering a more localized and disease-relevant vesicle population than fluid-derived exosomes.

Methods

Tissue samples (8 tumor/paracancer pairs) were digested enzymatically and processed using differential centrifugation, 0.22 µm filtration, and ultracentrifugation (110,000 × g).
Further purification was achieved via size-exclusion chromatography and ultrafiltration (100 kDa).
Exosomes were characterized by:
- Nano-flow cytometry: size range 50–200 nm
- TEM: classic cup-shaped morphology (~120 nm)
- Western blot: expression of CD9, CD63, TSG101; absence of non-exosomal marker GM130

Conclusion

This study demonstrates a reproducible method for isolating high-purity exosomes from solid tissue samples. The approach enabled subsequent proteomic profiling and highlighted the utility of tissue-derived exosomes as a reliable source for cancer biomarker discovery.

Exosome identification by NanoFCM, TEM, and Western blot showing size, morphology, and marker expression.

Figure 3. Exosome Identification and Characterization. (A) Overview of the experimental workflow. (B) NanoFCM confirms particle size distribution of isolated exosomes (50–200 nm). (C) TEM image reveals round exosome morphology (~120 nm); scale bar: 100 μm. (D) Western blot analysis detects exosomal markers CD9, CD63, TSG101, and absence of GM130 as a negative control. (Rao D, et al., 2023)

At Creative Biostructure, we are committed to helping you unlock the full potential of tissue-derived exosomes. Contact us to discuss your project needs or request a quote, and our experts are ready to support your research from isolation to in-depth functional analysis.