Exosome RNA Delivery
Are your RNA therapeutics being degraded by RNases before they even reach the target cell? Are you struggling with inefficient cellular uptake, endosomal entrapment, or off-target effects that compromise your gene therapy program?
Our specialized exosome platform is engineered to protect, transport, and deliver all major classes of RNA therapeutics. We transform fragile RNA molecules into stable, highly targeted drug candidates, ensuring they reach the cytoplasm of the correct cell and execute their function with maximum potency.
The Exosome Advantage for RNA Delivery
Lipid Nanoparticles (LNPs) are a common carrier, but they face significant challenges, including potential immunogenicity and dose-limiting toxicity. Exosomes, as the body's natural carriers, offer a superior alternative for complex RNA delivery.
- Superior Safety & Biocompatibility: Unlike synthetic LNPs, which can elicit pronounced cellular toxicity and inflammatory responses, exosomes are natural, biological nanovesicles. Studies (e.g., Tsai et al., 2021) have shown that exosomes have no adverse effects in vitro or in vivo at high doses, enabling repeat dosing.
- Lower Immunogenicity: As endogenous particles, exosomes are "invisible" to the immune system, preventing the rapid clearance and immune activation often seen with LNPs.
- Sustained Functional Delivery: Exosomes are not just a vehicle; they are a functional delivery system. Research shows exosome-delivered mRNA can drive sustained protein expression in vivo (e.g., for over 10 weeks) without signal attenuation, a feat difficult to achieve with LNP formulations.
- Inherent Targeting: Exosomes possess natural surface proteins that can facilitate uptake by specific recipient cells, a feature that must be synthetically "built-in" to LNPs.
Figure 1. Schematic representation of programmable exosomes for mRNAs loading. (Iqbal Z, et al., 2024)
Our Specialized RNA Delivery & Validation Capabilities
We have optimized protocols to handle the unique challenges of each RNA type. Our platform provides a tailored solution from loading to critical functional validation, ensuring your specific payload achieves its therapeutic goal.
| RNA Payload Type | Our Specialized Approach & Solution | Key Services Applied |
|---|---|---|
| siRNA & miRNA | High-Efficiency Silencing: We maximize loading efficiency and facilitate endosomal escape. Critically, we validate success in vitro via functional gene knockdown assays (e.g., qPCR, Western Blot) to confirm your target is silenced. | Cellular Functional Assays |
| mRNA | High-Fidelity Protein Expression: We protect large mRNA constructs to ensure delivery of the full-length, functional message. Success is validated by functional protein expression assays (e.g., Luciferase, GFP/RFP reporter assays) to prove your protein is being translated. | Dual-Luciferase Reporter Gene Assay |
| circRNA & lncRNA | Complex/Long-Lasting Regulation: We use gentle protocols to load these large, structured RNAs while maintaining their integrity. Success is validated by downstream functional assays specific to your target pathway or phenotype. | Exosomal Small RNA and miRNA Sequencing, In Vitro Exosome Functional Assays |
Core Technologies for Your RNA Program
We have built our platform around the core technical challenges of RNA delivery.
Optimized Nucleic Acid Loading
A successful delivery starts with efficient loading. We utilize a range of advanced methods, including proprietary techniques and electroporation, to actively load your specific RNA cargo into exosomes. Our process is optimized to maximize encapsulation efficiency while preserving the integrity and functionality of both the exosome and the delicate RNA payload.
Precision Targeting & Engineering
An RNA drug is useless if it silences the wrong cell. We engineer the exosome surface with specific targeting ligands—such as antibodies, peptides, or aptamers. This transforms the exosome into a "guided missile" that seeks out and delivers your RNA payload only to the cells that matter, dramatically increasing potency and reducing off-target toxicity.
Comprehensive Payload & Carrier Analytics
We provide full QC data so you know exactly what you are working with. Our analytics go beyond simple particle counting. We provide robust quantification of your loaded RNA, assess RNA integrity post-loading, and confirm the presence of targeting ligands on the vesicle surface, ensuring your final product is consistent, pure, and potent.
Application Spotlight: Silencing Addiction Pathways in the Brain
This analysis shows how to deliver siRNA across the blood-brain barrier (BBB) to treat complex neurological conditions, as demonstrated in landmark research.
Featured Technologies:
- Exosome Nucleic Acid Loading (siRNA)
- Exosome Surface Engineering (Targeting)
Literature Interpretation:
This paper addressed a major challenge: how to treat morphine relapse. The relapse behavior is driven by the Mu opioid receptor (MOR) gene in the brain, but this target is protected by the blood-brain barrier (BBB).
Researchers loaded exosomes with a specific siRNA designed to "silence" the MOR gene. To get this cargo past the BBB and deliver it only to neurons, they engineered the exosome surface with the RVG targeting peptide.
The results were a complete success: The RVG-targeted, siRNA-loaded exosomes successfully crossed the BBB, were taken up by neurons, and silenced the MOR gene in the target brain region. This precise gene knockdown dramatically suppressed morphine-seeking and relapse behaviors in animal models, validating this approach as a viable non-addictive therapy.
Figure 2. Confocal microscopy images showing selective uptake of RVG exosomes loaded with fluorescently labeled oligonucleotides in Neuro2A cells, but not in C2C12 cells. (Liu Y, et al., 2015)
Start Your RNA Delivery Project
We make getting started straightforward. Our process is designed to be collaborative and transparent, ensuring your project goals are met at every stage.
How It Works: Our Project Pathway
Figure 3. Project Workflow for Exosomes RNA Delivery Service. (Creative Biostructure)
Ready to solve your RNA delivery challenge? Our scientific team is available for a free consultation to discuss your specific RNA payload and therapeutic goals. Contact us today to discuss your project.
References
- Liu Y, Li D, Liu Z, et al. Targeted exosome-mediated delivery of opioid receptor Mu siRNA for the treatment of morphine relapse. Sci Rep. 2015 Dec 3;5:17543.
- Iqbal Z, Rehman K, Mahmood A, et al. Exosome for mRNA delivery: strategies and therapeutic applications J Nanobiotechnology. 2024 Jul 4;22(1):395.
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