Milk Exosome Lipidomics Analysis Service

Q: Q1. How many lipid species and classes can your service detect in milk exosomes?

A: Our UPLC-Orbitrap platform, with high-resolution mass accuracy (<5 ppm), covers over 1200 lipid species across 12 key classes and is specifically optimized for the unique characteristics of milk exosomes. Phospholipids (phosphatidylcholine PC, phosphatidylethanolamine PE, phosphatidylserine PS); Sphingolipids (sphingomyelin SM, ceramide Cer, ganglioside Gg); Neutral lipids (triacylglycerol TAG, cholesterol ester CE, diacylglycerol DAG); Fatty acids (free fatty acids FA, DHA/EPA-bound lipids). We also include 3-5 internal standards (e.g., C17-PC) for absolute quantification, ensuring CV <15% for most lipids.

Q: Q2: What quality control (QC) measures do you take to ensure data reliability?

A: We implement a 3-tier QC system to eliminate milk matrix interference and avoid result: Sample QC: Test pH (6.5-7.5 for normal milk), microbial load (<10³ CFU/mL), and hemolysis (<5%) upon receipt—reject samples that fail and notify you within 24h; Instrument QC: Run blank controls (to check reagent contamination) and lipid standard mixtures (to verify detection sensitivity) before each batch of samples; Data QC: Add 1 mixed QC sample (equal volumes of all test samples) for every 10 samples; lipid species with a CV greater than 30% in the QC samples are excluded from the final results.

Q: Q3: Do you support lipidomics analysis for low-fat/skimmed milk exosomes, and will the low-fat content affect the results?

A: Yes, we have a specialized protocol for low-fat/skimmed milk to avoid exosome loss: For low-fat milk (1-2% fat): We reduce initial centrifugation time from 15 min to 8 min (4℃, 3000×g) to prevent exosomes from co-precipitating with residual fat globules; For skimmed milk (<0.5% fat): We skip the fat-removal centrifugation step entirely and directly proceed to casein dissolution (using EDTA); this approach preserves over 90% of exosomes, compared to 75% with standard protocols. Lipid detection sensitivity and coverage for low-fat/skimmed milk are consistent with full-fat milk (1200+ species), ensuring no compromise on data quality.

Milk exosome lipidomics analysis enables the comprehensive profiling of functional lipids (such as phospholipids and sphingolipids) within milk exosomes. These lipids play a critical role in nutrient absorption, gut health, and the development of functional dairy products. Creative Biostructure offers professional milk exosome lipidomics analysis services. Leveraging advanced mass spectrometry and bioinformatics, we provide in-depth characterization of lipid compositions in milk exosomes, delivering scientific support for dairy R&D, functional food development, and nutritional research.

Why Analyze Lipids in Milk Exosomes?

Lipids go beyond their role as structural components of milk exosomes and are fundamental to their function. As natural nanocarriers, exosomes rely on their lipid membranes to protect and deliver bioactive molecules. Analyzing the lipid profile allows us to uncover their roles in nutrient absorption, immune modulation, and gut health. Furthermore, specific lipids, such as phospholipids and sphingolipids, serve as key mediators in cellular communication and metabolic regulation. By characterizing these lipids, we can enhance the development of functional dairy products, improve nutritional strategies, and unlock potential applications in targeted delivery systems. Understanding milk exosome lipids bridges the gap between nutrition and biotechnology, paving the way for smarter health solutions.

Protein and lipid composition of milk extracellular vesicles. Figure 1. Protein and lipid cargo of milk EVs. (Buratta, S. et al., 2023)

Our Milk Exosome Lipidomics Analysis Service

As essential mediators of intercellular communication, exosomes rely on their lipid components to play a pivotal role in nutrient delivery and bioactivity. We provide comprehensive milk exosome lipidomics analysis solutions to support your research and development needs.

Services	Description
Exosome Isolation and Purification	Using ultracentrifugation combined with density gradient centrifugation, we efficiently isolate exosomes from milk, ensuring high purity and integrity to establish a solid foundation for subsequent analysis. High-purity exosome isolation Particle size homogeneity analysis Validation of exosomal markers
Comprehensive Lipidomics Analysis	Using ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS), we comprehensively identify and quantify various lipid molecules in milk exosomes. Comprehensive lipid analysis (including phospholipids, triglycerides, etc.) Absolute and relative quantification analysis Discrimination of lipid isomers
Bioinformatics Analysis	Our specialized bioinformatics team delivers in-depth data analysis, including differential lipid screening, pathway enrichment analysis, and functional prediction. Integrated multi-omics data analysis Lipid-protein interaction network Visualized reports and interpretation

Workflow of Our Milk Exosome Lipidomics Analysis Service

We adhere to strict standardized operating procedures to ensure quality control at every step, delivering reliable experimental results and analytical data for you.

1

Sample Reception and Evaluation

We receive milk samples, conduct quality assessment and pretreatment, determine the optimal experimental protocol, and ensure the samples meet analytical requirements.

2

Exosome Isolation and Purification

Exosomes are isolated using ultracentrifugation combined with density gradient centrifugation, with their characteristics validated through Nanoparticle Tracking Analysis (NTA) and Western blot.

3

Lipid Extraction and Detection

Exosome lipids are extracted using the Bligh-Dyer method, followed by lipidomics analysis with ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS).

4

Data Analysis and Interpretation

Raw data processing, lipid identification and quantification are performed, followed by bioinformatics analysis to generate visualized results and comprehensive interpretation reports.

5

Report Delivery and Consultation

Detailed experimental reports and data analysis results are provided, complemented by consultations with our expert team for result interpretation and follow-up experimental design guidance.

Workflow for milk exosomal lipidomics analysis. Figure 2. Process for milk exosome lipidomics analysis. (Creative Biostructure)

Applications of Our Milk Exosome Lipidomics Analysis Service

Milk exosome lipidomics analysis services hold significant application value across multiple fields, providing robust support for scientific research and industrial development.

Dairy Product Development

By analyzing the impact of different processing techniques on the milk exosome lipid composition, we optimize dairy production processes to enhance the nutritional value and functional properties of products.

Functional Food Development

Exploring bioactive lipid molecules in milk exosomes provides a scientific basis for developing functional dairy products and nutritional supplements.

Nutrition Research

Investigate the role of milk exosome lipids in human digestion, absorption, and metabolic processes, elucidating their mechanisms of action on human health.

Animal Breeding

Analyzing differences in exosomal lipid composition among different dairy cow breeds to provide molecular markers and a theoretical foundation for selecting superior dairy breeds.

Food Safety Testing

Establishing a milk quality assessment and traceability system based on exosomal lipidomics to provide new technologies and methodologies for food safety regulation.

Biomedical Research

Investigating the potential of milk exosome lipids as drug carriers to provide a research foundation for developing novel targeted drug delivery systems.

Case Study

Case: Untargeted Lipidomics of Bovine, Goat, and Giant Panda Milk

Background

This study employed non-targeted lipidomics technology to conduct a comparative analysis of lipids in bovine milk (BM), goat milk (GM) and giant panda milk (GPM).

Research Highlights

A total of 19 lipid subclasses and 877 lipid molecules were identified in three species milk samples.
GPM was rich in phospholipids, including glycerophosphatidylcholine (PC), glycerophosphatidylethanolamine (PE) and glycerophosphatidylinositol (PI), while BM and GM were rich in diacylglycerol (DG) and triacylglycerol (TG).
Analysis revealed hundreds of SDLs between BM/GM and GPM in both ion modes. These lipids were implicated in 22 pathways, with sphingolipid, glycerophospholipid, and glycerolipid metabolism being the most significantly altered among the three milk species.

Conclusion

This work could provide comprehensive lipid information for GPM, BM and GM and might have important guiding significance for the research and development of GPM alternatives.

Comparative lipid analysis of bovine, goat, and giant panda milk. Figure 3. Lipid analysis in the bovine milk, goat milk, and giant panda milk. (Shu, Q. et al., 2025)

Creative Biostructure's milk exosome lipidomics analysis service delivers precise, in-depth lipid profiling to uncover the functional roles of milk exosomes, powering your research in nutrition, biotechnology, and therapeutic development. Benefit from our cutting-edge mass spectrometry tech, expert data analysis, and tailored workflows to accelerate discoveries. Contact us today to share your project goals and partner with specialists for reliable lipidomics results.

References

Buratta, S., et al. Protein and lipid content of milk extracellular vesicles: a comparative overview. Life. 13.2 (2023): 401.
Shu, Q., et al. Lipo-Nutritional Profile Analysis of Bovine Milk, Goat Milk and Giant Panda Milk Through Untargeted Lipidomics. Goat Milk and Giant Panda Milk Through Untargeted Lipidomics.

Frequently Asked Questions

For any inquiries, our support team is ready to help you get technical support for your research and maximize your experience with Creative Biostructure.

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