Exosome Isolation and Purification Services for Reliable Downstream Applications
Struggling to isolate clean, intact exosomes from complex samples? Creative Biostructure offers high-purity, customizable exosome isolation and purification services using ultracentrifugation, SEC, TFF, and other proven methods—ensuring consistency and performance for biomarker discovery, drug delivery, and formulation development.
Exosome Solutions for Your Proceeding Research
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Exosome Isolation Solutions Trusted by Global Leaders in Life Sciences
Creative Biostructure provides expert exosome isolation services trusted by academia, biotech, and pharma to support research and development.
What are Exosomes?
Exosomes are nanoscale extracellular vesicles (30-150 nm) secreted by most cell types. They carry proteins, lipids, mRNAs, and microRNAs, enabling intercellular communication. Due to their rich molecular cargo, exosomes hold great potential as disease biomarkers, tools for studying biological processes, and natural carriers for therapeutic delivery.
Why is Exosome Isolation Important for Downstream Applications?
Accurate exosome isolation is essential for research and development. Impurities like proteins or other vesicles can distort experimental results. High-purity exosomes ensure reliable data, reproducible outcomes, and valid biological insights. Optimized methods preserve exosome integrity and yield, enabling effective functional analysis, biomarker discovery, and drug delivery studies.
What Types of Samples Can We Help You Process
Creative Biostructure provides high-quality exosome isolation and purification services optimized for diverse research and industrial applications. Our protocols are tailored for different biological sources, ensuring high yield, purity, and functional integrity of isolated exosomes.
Isolate exosomes from adherent or suspension cells using ultracentrifugation, TFF, SEC, or precipitation. Ideal for production, mechanistic studies, and engineered vesicle research.
Extract high-purity exosomes from animal tissues using enzymatic digestion and gradient centrifugation. Supports preclinical and functional studies across diverse models.
Isolate exosomes from plasma, urine, saliva, CSF, and more. Optimized for biomarker discovery and omics studies in non-clinical, research-only settings.
Isolate plant exosomes from juices, saps, or extracts for use in nutraceuticals, cosmetic R&D, natural delivery systems, and cross-kingdom signaling studies.
Isolate extracellular vesicles from yeast or fungi for host-microbe interaction, antifungal response, and metabolic pathway analysis.
Obtain BEVs from gram-positive or gram-negative strains for microbiome studies, bacterial signaling, and antimicrobial research applications.
Isolate EVs from protozoan species for applications in host-pathogen interaction studies, vaccine development, and diagnostic biomarker discovery.
Leverage Diverse Isolation Technologies to Optimize Exosome Quality
Creative Biostructure offers a full range of exosome isolation methods tailored to sample type, yield requirements, and downstream application, ensuring purity, structural integrity, and reliable performance. We provide not only standalone exosome isolation methods but also integrated workflows that combine multiple techniques to meet your specific project goals and performance requirements.
| Method | Key Advantages | Best Use Cases | Sample Suitability | Purity Level |
|---|---|---|---|---|
| Ultracentrifugation | High purity and yield; standard for structural and functional exosome studies | Fundamental research, morphology and protein/RNA profiling | Cell culture, tissue, biofluids | ★★★★ |
| Size Exclusion Chromatography (SEC) | Gentle separation that preserves exosome integrity; removes protein/lipoprotein | Sensitive applications such as omics, diagnostics, and bioassays | Biofluids, conditioned media | ★★★★ |
| Polymer-Based Precipitation | Cost-effective and scalable; suitable for high-throughput and routine isolation | Initial screening and enrichment | Culture media, urine, plasma, serum | ★★ |
| Immunoaffinity Capture | High specificity for surface markers like CD9/CD63/CD81; isolates exosome enrichment | Targeted biomarker studies and therapeutic exosome research | Serum, plasma, engineered cells | ★★★★★ |
| Tangential Flow Filtration (TFF) | Scalable for large-volume processing; GMP-compatible and low shear | Biomanufacturing, drug delivery platform development | Large-scale cultures, bioreactor media | ★★★ |
| Microfluidics-Based Isolation | Fast, label-free isolation from small samples; high automation and precision | Precision biomarker discovery, microfluidic prototyping | Low-volume or rare samples | ★★★★ |
Tailored Exosome Isolation Services by Application Grade
Research-Grade Exosome Isolation
- • Supports mechanistic studies and omics analysis
- • Compatible with diverse sample types and workflows
- • Methods include ultracentrifugation, SEC, and precipitation
Diagnostic-Grade Exosome Purification
- • Ideal for biomarker discovery and liquid biopsy research
- • High-purity isolation from plasma, urine, CSF, etc.
- • Suitable for downstream qPCR, ELISA, and NGS assays
Exosome Purification for Drug Delivery Applications
- • Enables loading of RNA, proteins, or small molecules
- • Scalable isolation using SEC, TFF, and ultrafiltration
- • Supports early-stage therapeutic development
GMP-Compatible Exosome Isolation
- • Preclinical-ready workflows with documentation support
- • Scalable, low-shear purification for CMC studies
- • Suitable for IND-enabling and tech transfer pipelines
Creative Biostructure offers exosome isolation and purification services across multiple application grades. Whether you are conducting fundamental biological studies, exploring diagnostic biomarkers, or developing exosome-based therapeutics, we deliver solutions that align with your technical and regulatory needs.
Advanced Exosome Isolation and Purification Platforms
Creative Biostructure's exosome isolation platform features Beckman Coulter Optima ultracentrifuges, ÄKTA Pure SEC systems, TFF units, magnetic bead-based immunocapture, and automated microfluidic isolators. We ensure high purity and consistency with NTA, TEM, ELISA, Western blot, and DLS-based quality control.
Image Source: Beckman Coulter
Image Source: GE Healthcare Life Sciences
Image Source: Cytiva
How Does Our Exosome Isolation Workflow Work
At Creative Biostructure, our exosome isolation and purification workflow is designed to deliver high-quality, reproducible vesicles optimized for downstream research and development. Each project follows a modular and sample-specific process that integrates cutting-edge methods and rigorous quality control.
Sample Preparation and Pre-Processing
Debris removal and volume adjustment via low-speed centrifugation or filtration.
Exosome Isolation
Customized selection of methods such as ultracentrifugation, SEC, TFF, precipitation, or immunoaffinity capture based on sample type and application goals.
Exosome Purification and Concentration
Fine separation from non-exosomal components and volume reduction using gradient centrifugation, SEC columns, or TFF membranes.
Quality Control and Characterization
Comprehensive validation via NTA, TEM, DLS, Western blotting, and ELISA to assess size, morphology, surface markers, and purity.
Optional Downstream Processing
Includes exosome labeling, cargo loading, buffer exchange, or lyophilization, tailored to research or preclinical requirements.
Each step is carefully monitored by our expert team, ensuring traceable, consistent, and project-ready exosome preparations.
Starting a Project with Creative Biostructure
Sample Requirements
- • Cell Culture Supernatant: ≥10 mL of conditioned medium (serum-free or EV-depleted recommended)
- • Tissue Samples: ≥100 mg, fresh or frozen, stored at -80°C after snap freezing in liquid nitrogen
- • Biofluids: ≥1 mL per sample, transported on dry ice
- • Microbial or Plant Extracts: ≥5 mL clarified extract or lysate
- • Sample Packaging: Use 1.5 mL or 2 mL RNase/DNase-free microcentrifuge tubes, tightly sealed and clearly labeled
- • Transport Conditions: Ship samples on dry ice to preserve vesicle integrity; avoid freeze-thaw cycles
For other sample types or large-scale submissions, please contact our technical team for specific guidance.
Deliverables
- • High-purity exosome preparation in your selected buffer
- • Raw and processed data from NTA, DLS, or TEM (as applicable)
- • Western blot or ELISA results for marker validation (e.g., CD63, CD81)
- • Full experimental report including method, yield, and quality metrics
- • Optional services: buffer exchange, labeling, cargo loading, lyophilization
Our deliverables are designed to support downstream research, diagnostic development, or early-stage therapeutic formulation with full traceability and QC documentation.
Client Success Stories & Project Highlights
At Creative Biostructure, we deliver high-quality, customized exosome isolation and characterization services that support diverse research goals. Our clients rely on our expertise to generate reliable data for publications, product development, and translational research.
Case Study 1: Ultracentrifugation-Based Isolation and Characterization of Research Exosomes
A client approached Creative Biostructure with pre-isolated samples requiring standardized exosome purification and validation for molecular profiling and downstream assay development.
Our team applied a refined ultracentrifugation-based workflow using the Beckman L8-80M ultracentrifuge, followed by PBS washes and filtration steps, to remove residual protein aggregates and non-exosomal vesicles. The final pellet was resuspended and divided for quality control assays.
We performed transmission electron microscopy (TEM) to confirm morphology and size distribution, and NanoFCM particle size analysis revealed a homogenous population averaging ~82 nm. BCA protein quantification and RNA extraction further demonstrated the presence of functional molecular cargo. Sterility testing confirmed the sample's suitability for further biological use.
Transmission Electron Microscope Image of Exosomes. (Creative Biostructure)
Why Choose Our Exosome Isolation & Purification Services
At Creative Biostructure, we combine advanced technologies, customizable workflows, and stringent quality control to ensure your exosome research is accurate, scalable, and reproducible.
Comprehensive Technology Portfolio
We offer multiple validated isolation methods including ultracentrifugation, SEC, TFF, immunocapture, and microfluidics to suit a wide range of sample types and research needs.
High Purity and Reliable Results
Our optimized workflows preserve exosome integrity and molecular content, delivering consistent quality suitable for downstream analysis and experimental reproducibility.
Scalable and Customizable Solutions
From small-scale studies to preclinical process development, we tailor isolation strategies and service grades to align with your specific project goals and application requirements.
Advanced Instrumentation and QC Support
We utilize high-end platforms such as Beckman Optima ultracentrifuges, ÄKTA systems, and NanoFCM for characterization, supported by rigorous quality control and expert oversight.
What Our Customers Say
Frequently Asked Questions
For any inquiries, our support team is ready to help you get technical support for your research and maximize your experience with Creative Biostructure.
"We used their immunoaffinity-based isolation service for targeting neuron-derived exosomes from mouse CSF. The workflow was precise and reproducible, allowing us to profile disease-relevant biomarkers with confidence."
- Dr. Priya Mehta, Neuroscience Research Group
"We collaborated with Creative Biostructure to isolate plant-derived exosomes for use in a skincare formulation study. The vesicles showed excellent stability and bioactivity, and the team’s technical input was invaluable during formulation testing."
- Dr. Isabelle Laurent, R&D Manager
"We outsourced a large-volume exosome purification project to Creative Biostructure. Their ability to scale with TFF and maintain vesicle integrity was impressive. The QC reports were thorough and aligned well with our CMC documentation needs."
- Dr. Natalie Ho, Director of Preclinical Operations