Rodents Exosome Lipidomics Analysis Service

Q: Q1: What types of rodent samples and biofluids do you accept for exosome lipidomics analysis?

A: We support exosome lipidomics analysis for common laboratory rodents (mice, rats, hamsters, guinea pigs) and various sample types, including: Biofluids: Plasma (100 µL minimum), serum (100 µL minimum), urine (200 µL minimum), cerebrospinal fluid (CSF, 50 µL minimum), and ascites fluid (150 µL minimum); Tissues: Fresh/frozen tissue homogenates (50 mg minimum, e.g., liver, brain, tumor, adipose tissue); Cell culture supernatants: From rodent-derived cell lines (5 mL minimum, recommended to be concentrated before submission). All samples should be free of hemolysis or contamination to ensure lipid extraction quality.

Q: Q2: What lipid classes and species can your service detect, and what instruments do you use?

A: Our platform achieves comprehensive lipid coverage, detecting 12+ lipid classes and over 1,200 lipid species typical in rodent exosomes, including: Phospholipids (phosphatidylcholines, phosphatidylethanolamines, phosphatidylserines, etc.); Sphingolipids (sphingomyelins, ceramides, gangliosides); Neutral lipids (triacylglycerols, cholesterol esters, diacylglycerols); Fatty acids and their derivatives. We use state-of-the-art instruments: Thermo Scientific Orbitrap Exploris 480 (high-resolution LC-MS/MS) for accurate mass measurement (mass accuracy <5ppm) and Agilent 1290 UPLC for efficient separation of complex lipid mixtures, ensuring high sensitivity and reproducibility.

Q: Q3: Can you provide customized lipidomics analysis for specific research objectives (e.g., targeted lipid pathways)?

A: Yes, we offer fully customized analysis solutions. For example: If your research focuses on neurodegeneration (e.g., Alzheimer's mouse models), we can design a targeted panel for ceramide/sphingolipid metabolism; For metabolic disease studies (e.g., diabetic rat models), we can prioritize quantification of triacylglycerols and phospholipids related to insulin resistance. The customization process: 1) You share your research hypothesis and target pathways; 2) Our lipidomics experts design the detection strategy (e.g., selected reaction monitoring, SRM, for targeted lipids); 3) We validate the method with standard reference materials before sample analysis.

Q: Q4: What quality control measures do you implement to ensure data reliability?

A: We adhere to strict QC protocols throughout the workflow: Sample QC : Check sample integrity (e.g., hemolysis level, protein concentration) upon receipt; reject samples that fail quality standards and notify you promptly; Instrument QC : Run blank controls (to eliminate contamination) and standard lipid mixtures (to verify detection sensitivity) before each batch of samples; Data QC : Include 1 QC pool (mixed equal volumes of all samples) for every 10 test samples to monitor batch consistency; lipid species with CV >30% in QC pools are excluded from final results; Method validation : All analysis methods are validated for linearity (R² >0.99), precision (CV <15%), and recovery rate (80-120%) according to FDA/EMA bioanalytical guidelines.

Q: Q5: What factors affect the service cost, and do you offer academic discounts?

A: Pricing is determined by three key factors: Sample quantity: Discounts apply to large batches (e.g., 10+ samples: 10% off; 20+ samples: 20% off); Analysis type: Targeted lipid panels (focused on 1-2 lipid classes) are ~30% cheaper than comprehensive lipidomics; Additional services: Custom data analysis (e.g., integration with transcriptomics data) or technical replicates (3+ repeats per sample) incur extra fees. We offer 20% academic discounts for university laboratories, non-profit research institutions, and students (with valid institutional ID). For long-term collaborations (e.g., annual 50+ samples), we can negotiate a customized pricing package.

Q: Q6: After receiving the data, if we need to validate key lipid biomarkers, can you assist with follow-up experiments?

A: Yes, we provide post-analysis validation support to help translate lipidomics findings into actionable results: We can recommend suitable validation methods (e.g., Western blotting for lipid-related enzymes, ELISA for lipid metabolites, or targeted MS for specific lipid species); For clients who lack in-house validation capabilities, we offer a "validation service" (e.g., targeted MS confirmation of 5-10 candidate lipids) at a discounted rate; Our team can also assist in drafting the "Materials and Methods" section of your manuscript (for the lipidomics part) to ensure compliance with scientific publishing standards.

For scientists conducting preclinical research using rodents (mice and rats), a comprehensive understanding of the lipid composition of exosomes is a critical step toward elucidating disease mechanisms, discovering biomarkers, and evaluating drug efficacy. Creative Biostructure offers comprehensive lipidomics analysis services for rodent exosomes. Our services are specifically designed for exosome samples derived from rodent research subjects. We support advanced lipidomics analysis of rodent exosome samples, enabling comprehensive research into extracellular vesicle-mediated cell communication.

Why Analyze the Exosomal Lipidomics of Rodents?

Conducting exosomal lipidomics analysis in rodent models is a crucial step for delving into disease mechanisms and intercellular communication. As central models for studying human physiological and pathological processes, exosomes in rodents carry specific lipid "molecular fingerprints" of their parent cells. By analyzing their lipid profiles, we can non-invasively and dynamically reveal how lipid metabolism in cell membranes is reprogrammed during the development and progression of diseases (such as cancer, neurodegenerative disorders, or metabolic dysfunctions), thereby identifying potential disease biomarkers. Furthermore, exosomal lipids themselves play important roles in mediating membrane fusion, maintaining structural stability, and facilitating active signal transduction.

Therefore, this analysis not only contributes to breakthroughs in cutting-edge fundamental research but also provides critical insights for understanding drug efficacy and developing novel therapeutic strategies. It serves as a vital bridge, translating discoveries from rodent models into clinical medicine.

Lipidomic profiling of mouse adipocyte-derived extracellular vesicles (AdEVs). Figure 1. Analysis of the lipid signature in mouse adipocyte-derived extracellular vesicles (AdEVs). (Blandin, A. et al., 2023)

Our Rodents Exosome Lipidomics Analysis Service

Exosomes, as pivotal messengers in intercellular communication, carry a diverse array of glycoproteins that serve as sophisticated molecular codes for cell recognition and immune regulation. Creative Biostructure offers a specialized exosomal glycosylation analysis service to illuminate this hidden layer of biological information. Leveraging advanced lectin microarrays and mass spectrometry technologies, our service provides precise mapping of glycan structures and glycosylation sites, empowering clients to decipher disease-specific glycocodes and accelerate biomarker discovery.

Exosome Isolation & Purification
High-purity isolation of exosomes from various rodent biofluids using ultracentrifugation and size-exclusion chromatography.
- ≥95% exosome purity
- Contamination-free processing
- Quantification by NTA
Lipid Extraction & Profiling
Comprehensive lipid extraction and identification using state-of-the-art mass spectrometry techniques.
- Targeted and untargeted analysis
- Coverage of 10+ lipid classes
- High-resolution MS/MS analysis
Data Analysis & Interpretation
Advanced bioinformatics analysis and comprehensive reporting to facilitate biological interpretation.
- Multivariate statistical analysis
- Lipid pathway enrichment
- Customized data visualization

Workflow of Our Rodents Exosome Lipidomics Analysis Service

1

Exosome Isolation

The process begins with the isolation of exosomes from rodent samples.

2

Lipid Extraction and Separation

Lipids are then extracted from the exosomes and separated, often using chromatography techniques like liquid chromatography (LC).

3

Lipid Identification and Quantification

Advanced mass spectrometry (MS) platforms, including LC-MS/MS, are used to identify and quantify the different lipid species present.

4

Comprehensive Analysis

The service provides detailed lipid profiles, covering major lipid classes such as phospholipids, sphingolipids, and cholesterol.

5

Data Interpretation and Reporting

Services include comprehensive reporting with interactive data visualizations (e.g., heatmaps, PCA) and pathway analysis linked to databases like KEGG.

Lipidomic profiling pipeline for rodent exosomes. Figure 2. Workflow for lipidomic profiling of rodent exosomes. (Creative Biostructure)

Applications

Our rodent exosome lipidomics service supports a wide range of biomedical research areas.

Biomarker Discovery: Identifying specific lipid profiles that can serve as biomarkers for various diseases or conditions.
Understanding Disease Mechanisms: Gaining insights into how lipid composition influences cellular processes and disease progression.
Drug Delivery Development: Optimizing exosome lipid composition for targeted drug delivery by understanding their function as carriers.

Advanced Technology Platform

Our cutting-edge analytical instruments ensure high sensitivity, accuracy, and comprehensive lipid coverage for your rodent exosome samples.

High-Resolution Mass Spectrometry

Orbitrap-based LC-MS/MS systems for accurate mass measurement and reliable lipid identification.

Ultra-Performance Liquid Chromatography

UPLC systems with advanced column technology for superior separation of complex lipid mixtures.

Advanced Bioinformatics Pipeline

Custom-developed algorithms for lipid identification, quantification, and pathway analysis.

Case Study

Case: Lipidomic Analysis of Exosomes from Mouse Cortical Collecting Duct Cells

Background

The packaged material within exosomes includes proteins and lipids, but the molecular comparison within exosome subtypes is largely unknown. The purpose of this study was to investigate the molecular differences between two types of exosomes produced by mouse cortical collecting duct cells.

Research Highlights

Mass spectrometry-based shotgun lipidomics analysis showed significant differences in the lipid classes and fatty acid composition of the two types of exosomes.
Higher levels of sphingomyelin and lower levels of cardiolipin, among other phospholipids in the apical plasma membrane compared to the basolateral plasma membrane exosomes.

MS/MS-based profiling of fatty acid relative abundance in mpkCCD-derived exosomes. Figure 3. MS/MS spectra of the relative abundance of FAs within mpkCCD exosomes. (Dang, Viet D. et al., 2017)

Conclusion

Research findings indicate that the protein and lipid composition of exosomes derived from the apical and basolateral plasma membranes of renal collecting duct cells exhibits significant differences.

Creative Biostructure's rodents exosome lipidomics analysis service delivers precise, comprehensive lipid profiling to unlock insights into exosome-mediated mechanisms in rodent models. Leverage our advanced mass spectrometry platforms, expert data interpretation, and tailored analysis workflows to accelerate our client's research. Contact us today to discuss your project needs and kickstart high-quality lipidomics research with a trusted partner.

References

Dang, Viet D., et al. Lipidomic and proteomic analysis of exosomes from mouse cortical collecting duct cells. The FASEB Journal. 31.12 (2017): 5399.
Blandin, A., et al. Lipidomic analysis of adipose-derived extracellular vesicles reveals specific EV lipid sorting informative of the obesity metabolic state. Cell reports. 42.3 (2023).

Frequently Asked Questions

For any inquiries, our support team is ready to help you get technical support for your research and maximize your experience with Creative Biostructure.

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