Exosome Characterization and Analysis Services for Reliable Research Outcomes
Unsure how to best characterize your exosomes? Creative Biostructure offers comprehensive exosome characterization and analysis services compliant with MISEV2023 standards. Using NTA, TEM, flow cytometry, proteomics, and RNA analysis, we deliver accurate, reproducible insights for biomarker research and therapeutic development.
Exosome Solutions for Your Proceeding Research
Download Brochure
Exosome Characterization Expertise Trusted by Global Innovators
Creative Biostructure provides trusted exosome analysis services supporting universities, biotech, and pharma in advancing research and therapeutic innovation.
What Is Exosome Characterization and Why It Matters?
Exosomes are tiny vesicles released by cells that carry proteins, lipids, and nucleic acids reflecting their cellular origin. Through exosome analysis, researchers can explore how these particles influence disease mechanisms or serve as biomarkers. Because exosomes are diverse and sensitive to preparation conditions, proper exosome isolation and characterization are crucial for accurate results. Different isolation methods such as ultracentrifugation, size exclusion chromatography, or tangential flow filtration can alter the yield and purity of exosomes, affecting downstream data. Understanding these factors improves the consistency of exosomes research and supports the development of reliable diagnostic and therapeutic applications.
Comprehensive Exosome Characterization Solutions
Creative Biostructure provides comprehensive exosome characterization and analysis services that ensure accuracy, reproducibility, and MISEV2023 compliance, delivering precise insights into exosome identity, concentration, cargo, purity, and overall quality.
Identify exosomes through surface marker profiling, TEM morphology imaging, and NTA-based particle analysis. Suitable for validation, quality assessment, and functional studies.
Quantify exosome size and concentration using NTA and DLS to ensure measurement accuracy, data consistency, and reliable comparison across diverse sample types.
Profile exosomal proteins, lipids, and RNAs using proteomics, lipidomics, and RNA sequencing to uncover biological functions and diagnostic potential.
Ensure exosome integrity, reproducibility, and MISEV2023 compliance through standardized QC workflows, supporting reliable data for research and application development.
Explore Advanced Exosome Characterization Technologies
Creative Biostructure uses advanced exosome characterization technologies to generate high-resolution and reproducible data. Our methods reveal exosome morphology, surface markers, size, and molecular composition while ensuring accuracy, consistency, and full compliance with MISEV2023 standards.
| Category | Techniques | Key Advantages | Best Use Cases |
|---|---|---|---|
| Morphological Analysis | Transmission Electron Microscopy (TEM) | Provide direct visualization of exosome structure and integrity at nanometer scale. | Structural validation, morphology confirmation, and vesicle integrity assessment. |
| Cryo-Electron Microscopy (Cryo-EM) | |||
| Scanning Electron Microscopy (SEM) | |||
| Particle Size & Concentration Analysis | Nanoparticle Tracking Analysis (NTA) | Quantify exosome size distribution and concentration with high precision. | Quality control, comparative studies, and production consistency testing. |
| Dynamic Light Scattering (DLS) | |||
| Tunable Resistive Pulse Sensing (TRPS) | |||
| Surface Marker & Cargo Profiling | Flow Cytometry (FACS) | Identify exosomal surface proteins and internal cargo for functional and diagnostic insights. | Biomarker discovery, immunophenotyping, proteomics, and lipidomics studies. |
| Nano Flow Cytometry (NanoFCM) | |||
| Western Blotting | |||
| Mass Spectrometry | |||
| Advanced Biophysical & Imaging Techniques | Atomic Force Microscopy (AFM) | Offer nanoscale visualization and molecular fingerprinting for deeper characterization. | Mechanistic studies, exosome-cell interaction, and advanced imaging research. |
| TIRF Microscopy | |||
| Raman Spectroscopy | |||
| Super-Resolution Imaging |
Applications of Exosome Characterization and Analysis
Biomarker Discovery
- • Identifies disease-specific exosomal molecules
- • Supports omics and mechanism studies
- • Suitable for cancer and infection research
Drug Delivery System Development
- • Evaluates exosomes as natural drug carriers
- • Assesses stability and release behavior
- • Optimizes delivery system development
Regenerative Medicine
- • Studies stem cell-derived exosome functions
- • Supports tissue repair and wound healing
- • Applied in cardiac and skin regeneration
Oncology and Immunology
- • Profiles tumor and immune cell exosomes
- • Reveals signaling and resistance pathways
- • Aids cancer and immunotherapy research
By analyzing exosome composition and function, researchers can turn discoveries into practical applications. Creative Biostructure supports projects from discovery to applied research.
Advanced Exosome Analysis Platforms
Creative Biostructure's exosome characterization and analysis platforms are equipped with advanced instruments, including NanoSight NTA systems, NanoFCM analyzers, TEM and Cryo-EM units, Bruker AFM and Raman spectrometers, and high-resolution LC-MS/MS systems. These integrated platforms enable precise measurement of exosome morphology, size, surface markers, and molecular composition.
Image Source: Malvern Panalytical
Image Source: NanoFCM
Image Source: Thermo Fisher Scientific
Our Streamlined Exosome Characterization Workflow
At Creative Biostructure, our exosome characterization and analysis workflow is built to provide accurate, reproducible, and publication-ready data. Each project is customized to fit the client's research goals, integrating advanced analytical technologies and strict MISEV2023-compliant quality control.
Sample Preparation and Quality Assessment
Initial evaluation and pre-treatment to ensure sample integrity and remove unwanted impurities before analysis.
Physical and Morphological Characterization
Measurement of exosome size, shape, and concentration using NTA, DLS, TEM, or Cryo-EM for structural validation.
Molecular and Cargo Profiling
Comprehensive analysis of proteins, lipids, and RNAs through FACS, NanoFCM, Western blotting, and LC-MS/MS.
Purity and Quality Verification
Validation of exosome identity and purity with standardized QC assays ensuring data reliability and reproducibility.
Data Interpretation and Reporting
Delivery of detailed analytical reports with quantitative and visual data summaries to support downstream applications such as biomarker discovery or therapeutic development.
Creative Biostructure offers each exosome characterization technique as a standalone service or as part of an integrated, customizable analytical solution tailored to your research needs.
Starting a Project with Creative Biostructure
Sample Requirements
- • Minimum volume: 200-500 µL for biofluids or culture supernatants
- • Acceptable sample types: cell culture media, plasma, serum, urine, cerebrospinal fluid, plant extracts, or conditioned media
- • Storage and transport: samples should be frozen at -80°C and shipped on dry ice
- • Avoid repeated freeze-thaw cycles to preserve vesicle integrity
For special sample types or large projects, please consult our technical team for customized guidance.
Deliverables
- • Comprehensive analytical report including methods, raw data, and interpretation
- • Quantitative results for size, concentration, and purity
- • High-resolution TEM or Cryo-EM images (if applicable)
- • Protein, RNA, or lipid profiling data with annotated results
- • Quality control summary compliant with MISEV2023 guidelines
Our deliverables provide complete traceability and QC documentation, supporting advanced research, diagnostic innovation, and early therapeutic development.
Featured Exosome Characterization Case Study
At Creative Biostructure, we provide tailored exosome characterization and analysis solutions that empower researchers to obtain accurate, publication-ready data for research innovation.
Case Study: Comprehensive Characterization of Client-Supplied Exosomes
Creative Biostructure successfully completed a full-spectrum exosome characterization project for a client seeking detailed structural and molecular validation of their exosome samples. The study integrated multiple analytical techniques to ensure scientific accuracy.
Project Overview
- • Objective: To characterize client-provided exosomes for morphology, size, protein, and RNA content, as well as sterility verification.
- • Approach: Samples were processed through ultracentrifugation-based extraction, followed by sequential analytical evaluations.
Highlights of the Workflow
- • Ultracentrifugation: Efficiently isolated intact exosomes with minimal contaminants.
- • Transmission Electron Microscopy (TEM): Provided high-resolution visualization confirming spherical morphology and uniform structure.
- • Particle Size Analysis (NTA/DLS): Accurately measured exosome size and concentration before and after membrane treatment.
- • Protein Quantification (BCA): Determined total exosomal protein concentration for downstream assays.
- • RNA Extraction and Quantification: Successfully isolated high-quality RNA, confirmed via fluorometric analysis.
- • Sterility Testing: Verified that all samples were free from bacterial contamination, ensuring biosafety and experimental reliability.
Outcome
The client received a detailed analytical report including micrographs, quantitative measurements, and validated concentration data. The project confirmed exosome quality and integrity, enabling the client to confidently proceed with downstream biomarker and therapeutic research.
Exosome Particle Size Analysis Results. (Creative Biostructure)
Why Choose Our Exosome Characterization & Analysis Services
Creative Biostructure combines advanced technology, expert scientists, and MISEV2023 compliance to provide accurate exosome characterization results.
Comprehensive Analytical Capabilities
Access a full suite of validated techniques including NTA, TEM, Cryo-EM, AFM, FACS, and LC-MS/MS for complete exosome profiling.
Reliable and Reproducible Data
Our standardized workflows and QC checkpoints ensure consistency and reliability across all analytical results.
Customizable Service Options
Choose from individual analytical modules or integrated multi-tech solutions tailored to your specific research objectives.
Expert Team and Technical Support
Work with experienced scientists who provide end-to-end project guidance, from experimental design to data interpretation.
What Our Customers Say
Frequently Asked Questions
For any inquiries, our support team is ready to help you get technical support for your research and maximize your experience with Creative Biostructure.
"Creative Biostructure provided detailed, transparent exosome data with excellent TEM images and consistent results. Their scientific rigor and communication made them a trusted partner for our biomarker research."
– Dr. Emily Carter, Principal Investigator, Department of Molecular Medicine
"Their integrated workflow combining flow cytometry, LC-MS, and RNA analysis gave us reliable data for exosome-based drug studies. Flexible support and high-quality reports exceeded our expectations."
– Dr. Michael Huang, Senior Scientist, Translational Oncology
"Professional, precise, and compliant with MISEV standards. Their team ensured exosome integrity and documentation quality, helping us meet regulatory goals for regenerative medicine projects."
– Dr. Sarah Mitchell, Head of R&D