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Single (unilamellar) and oligolamellar lipid bilayer vesicles with large diameter have great potential in membrane study. Specifically, large unilamellar bilayer vesicles (with diameter >50 nm) permit the insertion of microelectrodes for measuring the electrical properties of lipid bilayer of different compositions. Those vesicles that are large enough to be observed by light microscopy can be fused with cells, and hence, used to study the mechanism of cell rotation in the presence of an alternating electrical field.
Rajiv Nayar et al. Generation of large unilamellar vesicles from long-chain saturated phosphatidylcholines by extrusion technique. Biochimica et Biophysica Acta, 986 (1989) 200-206
JOHN C MATHAI and V SITARAMAM. Preparation of Large Unilamellar Liposomes by the Ether Injection Method and Evaluation of the Physical Integrity by Osmometry. BIOCHEMICAL EDUCATION 15(3) 1987.
H. H. HUB et al. PREPARATION OF LARGE UNILAMELLAR VESICLES. Febs Letters, Volume 140, number 2. April 1982.