Baculovirus expression vector system (BEVS) has been widely used for the production of recombinant proteins in insect cells. It is one of the most powerful, robust, and versatile eukaryotic expression systems available. The BEVS is a helper-independent viral system which has been used to express heterologous genes from many different sources, including fungi, plants, bacteria and viruses, in insect cells.
Given its development speed and versatility for the expression of a wide range of protein families, the BEVS offers multiple advantages for protein production in a variety of applications. The large cloning capacity renders baculovirus an ideal tool for the synthesis of virus-like particles (VLP), which generally require simultaneous expression of multiple viral structural proteins for self-assembly.
Choosing the right system for foreign gene expression can be particularly important in obtaining biologically active recombinant protein. Several unique features of the BEVS have made it the system of choice for many applications.
Figure 1. High throughput screening process for baculovirus expression systems
Please see Creative Biostructure’s cell-free and cell-based expression services.