Creative Biostructure provides custom MemPro™ gene-to-structure services for Protein jagged. Our skilled scientists have successfully expressed and purified numerous membrane proteins, which greatly promoted customers’ research projects.
Figure 1. Human protein jagged-1 (JAG1).
Jagged proteins (JAG), a group of cell surface proteins that interact with four receptors in the mammalian Notch signaling pathway, modulating cell fate decisions during hematopoiesis. The family of Jagged proteins has five members, which function as ligands for multiple Notch receptors. The highly conserved Notch signaling pathway plays an important role in regulation of cell fate decisions in many organ systems. When JAG-Notch interaction happens in mammalian, a cascade of proteolytic cleavages is triggered leading to activation of the transcription for downstream target genes. Mammalian Jagged proteins (such as human JAG1) are the homologs of the Drosophilia jagged proteins. After the binding of protein jagged and NOTCH, a series of proteolytic cleavages take place and activate certain gene transcriptions. The JAG1 gene located on human chromosome 20 is expressed in multiple organ systems in the body. JAG1 is also defined as CD339 (cluster of differentiation 339). Protein jagged-1 and -2 (180 kDa and 150 kDa, respectively) belong to type I transmembrane glycoproteins, consisting of a small intracellular unit, a transmembrane region and an extracellular domain including DSL (delta, serrate, lag-2 consensus sequence) components and 15-16 calcium-binding EGF-like repeats. JAG1 mutation types include protein truncating (splice site, frameshift, and nonsense), missense, and whole gene deletions. Mutations of JAG can cause Alagille syndrome (ALGS), cancer or other diseases. ALGS is a multi-system disorder affecting several body systems, including the liver, heart, skeleton, eye, facial structure, kidneys and vascular system. Therefore, the study of JAG proteins is very crucial in clinical diagnosis and therapeutics.
Our experts from Creative Biostructure offer MemPro™ production, structural analysis and related services of recombinant Protein jagged.
Creative Biostructure can provide custom MemPro™ Membrane Protein Gene-to-Structure services, including the identification, isolation, purification, stabilization, and crystallization of membrane proteins of your interest. Please feel free to contact us for more information.
Chillakuri CR, et al. (2013). Structural analysis uncovers lipid-binding properties of Notch ligands. Cell Rep, 5(4): 861-867.
H. Yamamura, et al. (2014). Activation of Notch signaling by short-term treatment with Jagged-1 enhances store-operated Ca2+ entry in human pulmonary arterial smooth muscle cells. Am. J. Physiol. Cell Physiol., 306(9): C871-8.