Characterization and qualification of purified VLPs are crucial factors during VLPs production. Poor characterization can affect both VLPs design and VLPs vaccine development. The functionality of VLPs and the physical characters of VLPs including size and polydispersity are closely related to the efficacy and safety of VLPs vaccine. Creative Biostructure has developed a series comprehensive characterization methods to analysis our VLPs-based products from batch to batch manufacture, assuring to deliver the best services and products, including:
Figure 1. A range of Analytical tools for VLP characterization. (Biotechnology and Bioengineering, 2014)
We provide various Mempro™ Virus-like Particles (VLPs) Characterization services, also including Electron microscopy (TEM), Enzymatic NA activity assay, RP-HPLC, Western blot, LC-MS, ELISA, Surface Plasmon Resonance Immunoassay (SPR), Electrospray differential mobility analysis (ES-DMA), etc. Please feel free to contact us for a detailed quote.
C. M. Thompson, et al. (2013). Analytical technologies for influenza virus-like particle candidate vaccines: challenges and emerging approaches. Virology Journal,10: 141.
S. J. Kaczmarczyk, et al. (2009). Quantitative Characterization of Virus-like Particles by Asymmetrical Flow Field Flow Fractionation, Electrospray Differential Mobility Analysis, and Transmission Electron Microscopy. Biotechnology and Bioengineering, 102(3), 845–855.
L. H. L. Lua, et al. (2014). Bioengineering Virus-Like Particles as Vaccines.Biotechnology and Bioengineering , 111(3): 425-440.